The current strategies and underlying mechanisms in the control of the vector tick, Haemaphysalis longicornis: Implications for future integrated management

Nwanade, Chuks F.; Wang, Min; Li, Sisi; Yu, Zhijun; Liu, Jingze

doi:10.1016/j.ttbdis.2022.101905

Cited by 8 publications

(8 citation statements)

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“…Haemaphysalis longicornis , also known as the longhorned tick, is one of the most prevalent tick species in eastern Asia and an exotic species in Australia, New Zealand, and the United States [ 1 , 2 ]. It is a three-host tick that feeds on multiple hosts, including domestic and wild animals [ 3 ], and occasionally attacks humans [ 4 , 5 ]. Its veterinary and medical importance has been widely recognized because of its vector ability in transmitting a great diversity of pathogens, including severe fever with thrombocytopenia syndrome virus, Anaplasma phagocytophilum , Babesia microti , Ehrlichia chaffeensis , Rickettsia raoultii and Theileria orientalis [ 1 ], which can cause severe diseases in animals and humans [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

DNA Methylation Variation Is a Possible Mechanism in the Response of Haemaphysalis longicornis to Low-Temperature Stress

Nwanade

Wang

Bai

et al. 2022

IJMS

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Ticks are notorious ectoparasites and transmit the greatest variety of pathogens than any other arthropods. Cold tolerance is a key determinant of tick abundance and distribution. While studies have shown that DNA methylation is one of the important epigenetic regulations found across many species and plays a significant role in their response to low-temperature stress, its role in the response of ticks to low-temperature stress remains unexplored. Herein, we explored the DNA methylation profile of the tick, Haemaphysalis longicornis, exposed to low-temperature stress (4 °C) using whole-genome bisulfite sequencing (WGBS). We found that approximately 0.95% and 0.94% of the genomic C sites were methylated in the control and low-temperature groups, respectively. Moreover, the methylation level under the CG context was about 3.86% and 3.85% in the control and low-temperature groups, respectively. In addition, a total of 6087 differentially methylated regions (DMRs) were identified between the low-temperature and control groups, including 3288 hypermethylated and 2799 hypomethylated DMRs. Further, Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of differentially methylated genes revealed that most of the DMGs were significantly enriched in binding and RNA transport pathways. Taken together, this research confirmed, for the first time, the whole genome DNA methylation profile of H. longicornis and provided new insights into the DNA methylation changes relating to low-temperature stress in H. longicornis, as well as provided a foundation for future studies on the epigenetic mechanism underlying the responses of ticks to abiotic stress.

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Section: Introductionmentioning

confidence: 99%

DNA Methylation Variation Is a Possible Mechanism in the Response of Haemaphysalis longicornis to Low-Temperature Stress

Nwanade

Wang

Bai

et al. 2022

IJMS

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“…53 However, further understanding of the molecular mechanism underlying RNAi will deepen our knowledge of the involvement of sHsps in tick cold stress and may provide valuable information for the control of ticks. 1 Metabolism is crucial to maintain the balance and growth of insects, because cold stress below the optimal temperature adversely affects energy storage, metabolism, enzyme reaction rate, molecular transport, and diffusion in insects. [54][55][56] In D. melanogaster, energy reserves, such as amino acid proline and glycogen, affect cold tolerance.…”

Section: Discussionmentioning

confidence: 99%

“…Adult females are very important because of their reproductive potential in maintaining the tick populations. 1 For rapid cold treatment, unfed females were kept in a digital thermostatic bath at 4 °C or a sublethal temperature of −14 °C for 2 h. 10 The control group was maintained in a normal incubator (rearing temperature). All experiments involving rabbits were approved by The Animal Ethics Committee of Hebei Normal University (protocol number: IACUC-197516).…”

Section: Tick Collection and Cold Stressmentioning

confidence: 99%

“…The tick, Haemaphysalis longicornis Neumann, is an economically important pest of livestock and wildlife. 1 It is native to China, Japan, Korea, and the Primorsky Krai region of eastern Russia, 2 and is an exotic species in Australia, New Zealand, and the United States. 3,4 Since its discovery on a New Jersey farm in November 2017, H. longicornis has spread across several states in the United States owing to its wide host range and ability to reproduce parthenogenetically.…”

Section: Introductionmentioning

confidence: 99%

“…The tick, Haemaphysalis longicornis Neumann, is an economically important pest of livestock and wildlife 1 . It is native to China, Japan, Korea, and the Primorsky Krai region of eastern Russia, 2 and is an exotic species in Australia, New Zealand, and the United States 3,4 .…”

Section: Introductionmentioning

confidence: 99%

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Sequential expression of small heat shock proteins contributing to the cold response of Haemaphysalis longicornis (Acari: Ixodidae)

Pei,

Zhang,

Nwanade

et al. 2024

Pest Management Science

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BACKGROUNDHaemaphysalis longicornis is an important livestock pest and a serious threat to public health. Cold stress is a common form of stress affecting its survival and distribution. However, H. longicornis exhibits different physiological responses to cold stress. In this study, we systematically explored the regulation and functions of small heat shock proteins (sHsps) in H. longicornis during cold stress.RESULTSSeven sHsp genes (HlsHsp14.9, HlsHsp19.9, HlsHsp20.3, HlsHsp21.4, HlsHsp23.7, HlsHsp24.0, and HlsHsp26.1) with open reading frame lengths ranging from 408 bp (HlsHsp14.9) to 673 bp (HlsHsp26.1) were cloned from H. longicornis, which were featured with the typical α‐crystallin domain. Phylogenetic analysis revealed high similarity with the sHsps of arachnid species. Quantitative polymerase chain reaction revealed that the regulation of sHsp genes depended on the severity and duration of cold treatment. Moreover, the relative expression of each gene was largely dependent on the treatment periods (P < 0.01; 3, 6, and 9 d of treatment at 8, 4, 0, and –4 °C). Among all genes, HlsHsp14.9, HlsHsp19.9, HlsHsp20.3, and HlsHsp24.0 were most sensitive to rapid cold treatment. After RNA interference, the mortality of H. longicornis was significantly increased at –14 °C (P < 0.05), suggesting that the expression of sHsp genes is closely related to the cold tolerance of H. longicornis.CONCLUSIONOur results indicate that sHsps play an important role in the cold stress response of H. longicornis, which may enhance our understanding of the cold adaptation mechanisms in ticks.This article is protected by copyright. All rights reserved.

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Biochemical and molecular mechanisms involved in the response of Haemaphysalis longicornis (Acari: Ixodidae) to Cinnamomum cassia essential oil and its major constituent

Nwanade

Wang

et al. 2023

J Pest Sci

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The current strategies and underlying mechanisms in the control of the vector tick, Haemaphysalis longicornis: Implications for future integrated management

Cited by 8 publications

References 124 publications

DNA Methylation Variation Is a Possible Mechanism in the Response of Haemaphysalis longicornis to Low-Temperature Stress

DNA Methylation Variation Is a Possible Mechanism in the Response of Haemaphysalis longicornis to Low-Temperature Stress

Sequential expression of small heat shock proteins contributing to the cold response of Haemaphysalis longicornis (Acari: Ixodidae)

Biochemical and molecular mechanisms involved in the response of Haemaphysalis longicornis (Acari: Ixodidae) to Cinnamomum cassia essential oil and its major constituent

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