The cyanobacterial lectin, microvirin-N, enhances the specificity and sensitivity of lipoarabinomannan-based TB diagnostic tests

Horst, Megan van der; Karamchand, Leshern; Bauer, Westley S.; Nel, Andrew J. M.; Blackburn, Jonathan M.; Wright, David W.

doi:10.1039/d0an01725f

Cited by 4 publications

(2 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…11 According to relevant reports, LAM assay technology was mostly used for serum samples from immuno-compromised populations for TB infection at the early stage. 12,13 This study focused on the assay of LAM in morning urine from different populations. The sampling method was easy to grasp, the assay process was easy to operate, and the assay results could be quantitatively analyzed.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Validity of Urine LAM ELISA for Tuberculosis Infection

Junpeng,

Avoi,

Atil

2024

IJPQA

View full text Add to dashboard Cite

Objective: To explore the validity of urinary lipoarabinomannan (LAM) enzyme-linked immunosorbent assay (ELISA) assay technology for detecting MTB infection in the double infection of acquired immunodeficiency syndrome (AIDS) and human immunodeficiency virus-tuberculosis (HIV-TB) population with sputum-producing problems, and to explore the background value and medical reference value range of urinary LAM in the general population and people living with human immunodeficiency virus (HIV) or patients with acquired immunodeficiency syndrome (HIV/AIDS population). Method: About 307 individuals from the general population group, HIV/AIDS population group, TB population group and HIV-TB population group provided by Seventh Hospital of Tangshan city were selected for early morning urine analysis. LAM ELISA competition method and double antibody sandwich method were used to detect the concentration of LAM in urine. Standard curves of LAM optical density OD value were drawn. The differences in LAM concentration in different groups of urine were calculated, and the diagnostic validity of LAM ELISA techniques was explored. Result: (1) The corresponding curve formula of the ELISA competition method was y = 1.696-0.087x+3.100/x2; The corresponding curve formula for the double antibody sandwich method was y = -0.205+0.587x-0.097x2+0.001x3. (2) In LAM ELISA competition method, the difference in LAM OD values between the TB population group and the general population group was statistically significant (t = 3.393, p &LT; 0.05), and the difference in LAM OD values between the HIV-TB population group and the HIV/AIDS population group was statistically significant (t = 2.294, p &LT; 0.05); The difference in LAM concentration between TB population group and general population group was statistically significant (t = -4.642, p &LT; 0.05), and the difference in LAM concentration between HIV-TB population group and HIV/AIDS population group was statistically significant (t = -4.737, p &LT; 0.05). In LAM ELISA double antibody sandwich method, there was a statistically significant difference in LAM OD values between TB population group and the general population group (t = -2.566, p &LT; 0.05), and there was a statistically significant difference in LAM OD values between HIV-TB population group and HIV/AIDS population group (t = -3.212, p &LT; 0.05); The difference in LAM concentration between TB population group and general population group was statistically significant (t = -5.722, p &LT; 0.05), and the difference in LAM concentration between HIV-TB population group and HIV/AIDS group was statistically significant (t = -8.118, p &LT; 0.05). (3) Receiver operating characteristic curve (ROC) curve analysis showed that in the LAM ELISA competition method, the SPE of TB infection in the HIV-TB population group diagnosed with urine LAM was higher than those in TB population group, with a statistically significant difference (F = 31.227, p &LT; 0.05). Compared to the general population group, LAM ELISA competition method SEN in TB population group was lower than that in the ELISA double antibody sandwich method, and the difference was statistically significant (F = 15.667, p &LT; 0.05). Conclusion: The validity of urine LAM ELISA technology in the HIV-TB population group with TB infection was better than that in TB population group, and the validity of the LAM ELISA double antibody sandwich method was better than that in ELISA competitive method. The feasibility of urine LAM ELISA technology in HIV-TB was worthy of recognition, and the technology could be further improved and promoted.

show abstract

Section: Discussionmentioning

confidence: 99%

Analysis of the Validity of Urine LAM ELISA for Tuberculosis Infection

Junpeng,

Avoi,

Atil

2024

IJPQA

View full text Add to dashboard Cite

show abstract

“…[8,[16][17][18][19] Notable advances have been made in state-of-the-art electronic biosensors, including electrochemiluminescence sensors, [20,21] field-effect transistor (FET)-based sensors, [22][23][24] interdigital electrode piezoelectric quartz crystal sensors, [25,26] and impedimetric sensors. [17,27,28] Among them, FET-based biosensors exhibit major advantages such as labelfree and sensitive detection, high integrability, scalable production, signal amplification ability, and a short turn-around time. [16,23,29] In terms of Mtb detection, FET biosensors have achieved a limit of detection (LoD) of ≈2.1 × 10 −10 g mL −1 (=2.3 × 10 6 CFU mL −1 ) antigen protein in phosphate buffered saline buffer.…”

Section: Introductionmentioning

confidence: 99%

Accurately Detecting Trace‐Level Infectious Agents by an Electro‐Enhanced Graphene Transistor

Dai

Yang

Xiong

et al. 2023

Adv Funct Materials

View full text Add to dashboard Cite

For epidemic prevention and control, molecular diagnostic techniques such as field-effect transistor (FET) biosensors is developed for rapid screening of infectious agents, including Mycobacterium tuberculosis, SARS-CoV-2, rhinovirus, and others. They obtain results within a few minutes but exhibit diminished sensitivity (<75%) in unprocessed biological samples due to insufficient recognition of low-abundance analytes. Here, an electro-enhanced strategy is developed for the precise detection of trace-level infectious agents by liquid-gate graphene field-effect transistors (LG-GFETs). The applied gate bias preconcentrates analytes electrostatically at the sensing interface, contributing to a 10-fold signal enhancement and a limit of detection down to 5 × 10 −16 g mL −1 MPT64 protein in serum. Of 402 participants, sensitivity in tuberculosis, COVID-19 and human rhinovirus assays reached 97.3% (181 of 186), and specificity is 98.6% (213 of 216) with a response time of <60 s. This study solves a long-standing dilemma that response speed and result accuracy of molecular diagnostics undergo trade-offs in unprocessed biological samples, holding unique promise in high-quality and population-wide screening of infectious diseases.

show abstract

Microbial lectins as a potential therapeutics for the prevention of certain human diseases

Bektas,

Kaptan

2024

Life Sciences

View full text Add to dashboard Cite

The cyanobacterial lectin, microvirin-N, enhances the specificity and sensitivity of lipoarabinomannan-based TB diagnostic tests

Abstract: The lectin microvirin was used for the first time to selectively bind the tuberculosis biomarker ManLAM.

Cited by 4 publications

References 39 publications

Analysis of the Validity of Urine LAM ELISA for Tuberculosis Infection

Analysis of the Validity of Urine LAM ELISA for Tuberculosis Infection

Accurately Detecting Trace‐Level Infectious Agents by an Electro‐Enhanced Graphene Transistor

Microbial lectins as a potential therapeutics for the prevention of certain human diseases

Contact Info

Product

Resources

About