The cyclic-di-GMP diguanylate cyclase CdgA has a role in biofilm formation and exopolysaccharide production in Azospirillum brasilense

Ramírez-Mata, Alberto; López-Lara, Lilia I.; Xiqui-Vázquez, Ma. Luisa; Jijón-Moreno, Saúl; Romero-Osorio, Angelica; Baca, Beatriz E.

doi:10.1016/j.resmic.2015.12.004

Cited by 27 publications

(24 citation statements)

References 40 publications

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“…noeJ and noeL Lipopolysaccharide and EPS synthesis [276] cdgA Encodes diguanylate cyclase A (CdgA) involved in biofilm formation and EPS production [277] Azospirillum brasilense Sp245 nirK (nirK1 and nirK2) Encodes a Nitric oxide producing nitrite reductase involved in biofilm formation and root colonization [278,279] flhB1…”

Section: Genes Involved In Biofilm Formationmentioning

confidence: 99%

Agriculturally important microbial biofilms: Present status and future prospects

2017

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Microbial biofilms are a fascinating subject, due to their significant roles in the environment, industry, and health. Advances in biochemical and molecular techniques have helped in enhancing our understanding of biofilm structure and development. In the past, research on biofilms primarily focussed on health and industrial sectors; however, lately, biofilms in agriculture are gaining attention due to their immense potential in crop production, protection, and improvement. Biofilms play an important role in colonization of surfaces - soil, roots, or shoots of plants and enable proliferation in the desired niche, besides enhancing soil fertility. Although reports are available on microbial biofilms in general; scanty information is published on biofilm formation by agriculturally important microorganisms (bacteria, fungi, bacterial-fungal) and their interactions in the ecosystem. Better understanding of agriculturally important bacterial-fungal communities and their interactions can have several implications on climate change, soil quality, plant nutrition, plant protection, bioremediation, etc. Understanding the factors and genes involved in biofilm formation will help to develop more effective strategies for sustainable and environment-friendly agriculture. The present review brings together fundamental aspects of biofilms, in relation to their formation, regulatory mechanisms, genes involved, and their application in different fields, with special emphasis on agriculturally important microbial biofilms.

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Section: Genes Involved In Biofilm Formationmentioning

confidence: 99%

Agriculturally important microbial biofilms: Present status and future prospects

2017

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“…Aerotaxis is the strongest behavioral response in A. brasilense. Of 33 putative diguanylate cyclases and 25 phosphodiesterases encoded by the A. brasilense genome (10,11), only 2, ChsA and CdgA, have thus far been functionally characterized (11,12), and ChsA has been shown to contribute to oxygen-induced changes in intracellular c-di-GMP levels (5).…”

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confidence: 99%

Optogenetic Manipulation of Cyclic Di-GMP (c-di-GMP) Levels Reveals the Role of c-di-GMP in Regulating Aerotaxis Receptor Activity in Azospirillum brasilense

et al. 2017

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Bacterial chemotaxis receptors provide the sensory inputs that inform the direction of navigation in changing environments. Recently, we described the bacterial second messenger cyclic di-GMP (c-di-GMP) as a novel regulator of a subclass of chemotaxis receptors. In Azospirillum brasilense, c-di-GMP binds to a chemotaxis receptor, Tlp1, and modulates its signaling function during aerotaxis. Here, we further characterize the role of c-di-GMP in aerotaxis using a novel dichromatic optogenetic system engineered for manipulating intracellular c-di-GMP levels in real time. This system comprises a red/near-infrared-light-regulated diguanylate cyclase and a blue-light-regulated c-di-GMP phosphodiesterase. It allows the generation of transient changes in intracellular c-di-GMP concentrations within seconds of irradiation with appropriate light, which is compatible with the time scale of chemotaxis signaling. We provide experimental evidence that binding of c-di-GMP to the Tlp1 receptor activates its signaling function during aerotaxis, which supports the role of transient changes in c-di-GMP levels as a means of adjusting the response of A. brasilense to oxygen gradients. We also show that intracellular c-di-GMP levels in A. brasilense change with carbon metabolism. Our data support a model whereby c-di-GMP functions to imprint chemotaxis receptors with a record of recent metabolic experience, to adjust their contribution to the signaling output, thus allowing the cells to continually fine-tune chemotaxis sensory perception to their metabolic state.IMPORTANCE Motile bacteria use chemotaxis to change swimming direction in response to changes in environmental conditions. Chemotaxis receptors sense environmental signals and relay sensory information to the chemotaxis machinery, which ultimately controls the swimming pattern of cells. In bacteria studied to date, differential methylation has been known as a mechanism to control the activity of chemotaxis receptors and modulates their contribution to the overall chemotaxis response. Here, we used an optogenetic system to perturb intracellular concentrations of the bacterial second messenger c-di-GMP to show that in some chemotaxis receptors, c-di-GMP functions in a similar feedback loop to connect the metabolic status of the cells to the sensory activity of chemotaxis receptors.

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“…LapA has domains resembling adhesins involved in biofilm formation of Gram-positive bacteria, and a domain similar to Ca 2+ binding proteins and haemolysins, found to be involved in host-cell interactions (Hinsa and O'Toole 2006). More recently, Ramírez-Mata et al (2016) investigated that cyclic-di-GMP diguanylate cyclase CdgA play an important role in attachment and subsequent biofilm or microcolonies formation in A. brasilense.…”

Section: Quorum Sensing: Cell Density Dependent Colonizationmentioning

confidence: 99%

Mechanistic insights on plant root colonization by bacterial endophytes: a symbiotic relationship for sustainable agriculture

Jha

Panwar

2018

Environmental Sustainability

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Plant-microbe and soil interactions are one of the oldest muse for multi-disciplinary researchers. Plant growth promoting microorganisms influence the host physiology by secreting regulatory chemical signals in the vicinity of plant roots and play a key role in the enhancement of plant growth and expansion. The present review deals with the in-depth understanding of steps involved in host tissues colonization by bacterial endophytes. The molecular insights of these events, particularly for endophytic bacteria, are poorly documented till date. The endophytic bacteria must coexist with the host plant and capable of colonizing the internal plant tissues without being recognized as a pathogen. A proper understanding of exchange of signals between the host plant and bacterial communities is required which may facilitate the development of new strategies to promote beneficial interactions between them. This knowledge can be instrumental in agricultural practices as well as for phytoremediation of pollutants. Keeping these facts in mind, the present review attempts to explore the systematic understanding of steps involved and molecular insights of plant colonization events by endophytic bacteria. We conclude that molecular mechanisms and factors affecting endophytic bacterial colonization deserve more research attention in order to exploit their beneficial aspects for sustainable agriculture and environment.

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The cyclic-di-GMP diguanylate cyclase CdgA has a role in biofilm formation and exopolysaccharide production in Azospirillum brasilense

Cited by 27 publications

References 40 publications

Agriculturally important microbial biofilms: Present status and future prospects

Agriculturally important microbial biofilms: Present status and future prospects

Optogenetic Manipulation of Cyclic Di-GMP (c-di-GMP) Levels Reveals the Role of c-di-GMP in Regulating Aerotaxis Receptor Activity in Azospirillum brasilense

Mechanistic insights on plant root colonization by bacterial endophytes: a symbiotic relationship for sustainable agriculture

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