The cytogenetic action of ifosfamide, mesna, and their combination on peripheral rabbit lymphocytes: an in vivo/in vitro cytogenetic study

Abstract

Ifosfamide (IFO) is an alkylating nitrogen mustard, administrated as an antineoplasmic agent. It is characterized by its intense urotoxic action, leading to hemorrhagic cystitis. This side effect of IFO raises the requirement for the co-administration with sodium 2-sulfanylethanesulfonate (Mesna) aiming to avoid or minimize this effect. IFO and Mesna were administrated separately on rabbit's lymphocytes in vivo, which were later developed in vitro. Cytogenetic markers for sister chromatid exchanges (SCEs), proliferation rate index (PRI) and Mitotic Index were recorded. Mesna's action, in conjunction with IFO reduces the frequency of SCEs, in comparison with the SCEs recordings obtained when IFO is administered alone. In addition to this, when high concentrations of Mesna were administered alone significant reductions of the PRI were noted, than with IFO acting at the same concentration on the lymphocytes. Mesna significantly reduces IFO's genotoxicity, while when administered in high concentrations it acts in an inhibitory fashion on the cytostatic action of the drug.

In vivo anticlastogenic effect of silymarin from milk thistle Silybum marianum L.

“…At 19 h after BrdU tablet insertion, each animal was injected with 0.1 mL Colcemid (20 mg) intravenously, in the tail vain. Slides were stained for 10 min in the dark[ 19 ] using 50 μ/mL Hoechst dye 33258, prepared in distilled water at pH 7. The preparation was rinsed in distilled water before adding a layer of Mcilvaine's buffer (18 mL of citric acid [1.92%] added to 82 mL of disodium phosphate [2%] and adjusted to pH 7).…”

A clinical and histopathological comparison of saline, adrenaline and 2-mercaptoethanesulfonate (MESNA) in mucoperichondrial elevation: which is superior?

“…Mesna, which is the sulphur salt of 2-mercaptoethanolsulphamic acid, received a patent for ‘chemical assisted dissection’ and enables de-adhesion between pathological and healthy tissues, thus facilitating healthy tissues in dissociating from the surrounding tissues. Therefore, numerous surgical branches, from otolaryngology to gynaecology and orthopaedics, use mesna for intraoperative chemical dissection 13 , 14 .…”