The Cytokine Interleukin-6 and the Chemokines CCL20 and CXCL13 Are Novel Biomarkers of Specific Endogenous Uveitic Entities

El‐Asrar, Ahmed M. Abu; Berghmans, Nele; Al-Obeidan, Saleh A.; Mousa, Ahmed; Opdenakker, Ghislain; Damme, Jozef Van; Struyf, Sofie

doi:10.1167/iovs.16-19758

Cited by 41 publications

(37 citation statements)

References 64 publications

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“…In the present study, we confirmed our previous data (Abu El‐Asrar et al. ) in a new set of AH samples with the use of another multiplex assay. Furthermore, the levels of CCL20 were elevated 465‐fold and 148‐fold in patients with HLA‐B27‐associated uveitis and BD, respectively, compared with controls.…”

Section: Discussionsupporting

confidence: 91%

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The CC chemokines CCL8, CCL13 and CCL20 are local inflammatory biomarkers of HLA‐B27‐associated uveitis

El‐Asrar

Berghmans

Al-Obeidan

et al. 2018

Acta Ophthalmologica

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Purpose: To determine the concentrations of the CC chemokines CCL2, CCL7, CCL8, CCL11, CCL13, CCL20, CCL24 and CCL26 in aqueous humour (AH) samples from patients with specific uveitic entities. Methods: Aqueous humour samples from patients with active uveitis associated with Behc ßet's disease (BD) (n = 13), sarcoidosis (n = 8), HLA-B27-related inflammation (n = 12), Vogt-Koyanagi-Harada (VKH) disease (n = 12) and control patients (n = 9) were assayed with the use of a multiplex assay. Results: When considering all uveitis patients as one group, all chemokine levels except CCL2 were significantly increased compared to controls. CCL8, CCL13 and CCL20 were the most strongly upregulated, 48-fold, 118-fold and 173-fold, respectively, above control AH levels. CCL8 and CCL13 levels were significantly higher in HLA-B27-associated uveitis than in sarcoidosis and VKH disease. CCL20 levels were significantly higher in HLA-B27-associated uveitis than in BD, sarcoidosis and VKH disease. In addition, CCL20 levels were significantly higher in BD than in VKH disease. In HLA-B27-associated uveitis, CCL8, CCL13 and CCL20 were upregulated 111-fold, 255-fold and 465-fold, respectively, compared with controls. CCL8, CCL13 and CCL20 levels were significantly higher in nongranulomatous uveitis (BD and HLA-B27-associated uveitis) than in granulomatous uveitis (sarcoidosis and VKH disease). Conclusion: Immune responses mediated by CCL8, CCL13 and CCL20 appear to be more potent in nongranulomatous uveitis, particularly in HLA-B27associated uveitis.

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Section: Discussionsupporting

confidence: 91%

“…; Abu El‐Asrar et al. ). The wide range in clinical manifestations and the varied response to therapy leave multiple challenges in uveitis management.…”

Section: Introductionmentioning

confidence: 99%

The CC chemokines CCL8, CCL13 and CCL20 are local inflammatory biomarkers of HLA‐B27‐associated uveitis

El‐Asrar

Berghmans

Al-Obeidan

et al. 2018

Acta Ophthalmologica

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“…CCL17 also could be inhibited by overexpression of SOCS1 in the retina to regulate the recruitment of inflammatory cells [44]. The cytokine CCL20 was considered to be a specific biomarker of HLA-B27 -associated uveitis [45]. Our study revealed that CCL4 , CCL17 and CCL20 likely play essential roles in uveitis.…”

Section: Resultsmentioning

confidence: 85%

Determination of Genes Related to Uveitis by Utilization of the Random Walk with Restart Algorithm on a Protein–Protein Interaction Network

Yan

et al. 2017

IJMS

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Uveitis, defined as inflammation of the uveal tract, may cause blindness in both young and middle-aged people. Approximately 10–15% of blindness in the West is caused by uveitis. Therefore, a comprehensive investigation to determine the disease pathogenesis is urgent, as it will thus be possible to design effective treatments. Identification of the disease genes that cause uveitis is an important requirement to achieve this goal. To begin to answer this question, in this study, a computational method was proposed to identify novel uveitis-related genes. This method was executed on a large protein–protein interaction network and employed a popular ranking algorithm, the Random Walk with Restart (RWR) algorithm. To improve the utility of the method, a permutation test and a procedure for selecting core genes were added, which helped to exclude false discoveries and select the most important candidate genes. The five-fold cross-validation was adopted to evaluate the method, yielding the average F1-measure of 0.189. In addition, we compared our method with a classic GBA-based method to further indicate its utility. Based on our method, 56 putative genes were chosen for further assessment. We have determined that several of these genes (e.g., CCL4, Jun, and MMP9) are likely to be important for the pathogenesis of uveitis.

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“…33 In the present study, the concentrations of CXCL13 in the AH were quantified in a new set of AH samples. The results were consistent with and confirmed our previous data in that higher concentrations of CXCL13 levels were measured in AH from patients with VKH disease.…”

Section: Discussionmentioning

confidence: 96%

Differential CXC and CX3C Chemokine Expression Profiles in Aqueous Humor of Patients With Specific Endogenous Uveitic Entities

El‐Asrar

Berghmans

Al-Obeidan

et al. 2018

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. To determine the levels of the neutrophil chemoattractants CXCL1, CXCL2, CXCL5, CXCL6, and CXCL8, the T helper 1 chemoattractants CXCL9, CXCL10 and CXCL11, the lymphoid chemokines CXCL12 and CXCL13 and the soluble form of the transmembrane chemokines CXCL16 and CX3CL1, in aqueous humor samples from patients with specific uveitic entities.METHODS. Aqueous humor samples from patients with active uveitis associated with Behçet's disease (n ¼ 13), sarcoidosis (n ¼ 8), HLA-B27-related inflammation (n ¼ 12), Vogt-KoyanagiHarada (VKH) disease (n ¼ 12), and healthy controls (n ¼ 9) were assayed with the use of a multiplex assay.RESULTS. All chemoattractant levels were significantly higher in all patients than in the controls. The levels of all neutrophil chemoattractants and CXCL10, CXCL16, and CX3CL1 were significantly higher in nongranulomatous uveitis (Behçet's disease and HLA-B27-associated uveitis) than in granulomatous uveitis (sarcoidosis and VKH disease), whereas the levels of the B cell chemoattractant CXCL13 were significantly higher in granulomatous uveitis than in nongranulomatous uveitis. CXCL13 levels were highest in the patients with VKH disease. CXCL9, CXCL11, and CXCL12 levels did not differ significantly.CONCLUSIONS. Inflammation in nongranulomatous uveitis appears to be driven by neutrophils and T helper 1 lymphocytes, whereas B lymphocytes may contribute to the inflammatory process in granulomatous uveitis, particularly in VKH disease.

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The Cytokine Interleukin-6 and the Chemokines CCL20 and CXCL13 Are Novel Biomarkers of Specific Endogenous Uveitic Entities

Cited by 41 publications

References 64 publications

The CC chemokines CCL8, CCL13 and CCL20 are local inflammatory biomarkers of HLA‐B27‐associated uveitis

The CC chemokines CCL8, CCL13 and CCL20 are local inflammatory biomarkers of HLA‐B27‐associated uveitis

Determination of Genes Related to Uveitis by Utilization of the Random Walk with Restart Algorithm on a Protein–Protein Interaction Network

Differential CXC and CX3C Chemokine Expression Profiles in Aqueous Humor of Patients With Specific Endogenous Uveitic Entities

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