The cytokine response in THP-1 (monocyte) and HL-60 (neutrophil-differentiated) cells infected with different genotypes of Helicobacter pylori strains

Caliskan, Reyhan; Sayi-Yazgan, Ayca; Tokman, Hrisi Bahar; Sofyalı, Emre; Erzın, Yusuf; Akgül, Özer; Kurt, Aykut; Kalaycı, Fatma; Ziver, Tevhide; Yüksel, Pelin; Bal, Kadir; Kocazeybek, Bekir

doi:10.5152/tjg.2015.8058

Cited by 11 publications

(7 citation statements)

References 45 publications

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“…According to the result, the expressions of IL-8 increased significantly after the stimulation of H. pylori . The overall effect of the stimulation of H. pylori was consistent with previous findings [39]. The production of different cytokines could be induced by the infiltration of immunocyte (such as T lymphocytes, monocyte and neutrophils) into diseased tissues [40–42].…”

Section: Discussionsupporting

confidence: 88%

Effect ofHelicobacter pyloriinfection on chronic periodontitis by the change of microecology and inflammation

Zhang

et al. 2016

Oncotarget

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Helicobacter pylori (H. pylori), a pathogen inducing peptic disease, is recently found to be binding to the progress of periodontitis. Most previous studies are case-controlled, and they investigate the risk of H. pylori infection in disease the development of while few studies evaluate the correlation between H. pylori and periodontal pathogens. Therefore, we investigated the correlation between H. pylori infection with periodontal parameters, periodontal pathogens and inflammation. The results indicated that patients with H. pylori showed significantly higher probing depth and attachment loss than those without (p < 0.05). Among 28 subgingival plaque samples from 14 patients, the frequencies of Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum and Treponema denticola were significantly higher with H. pylori infection than those without H. pylori infection (p < 0.05). However, the frequency of Aggregatibacter actinomycetemcomitans was lower (p < 0.05). Furthermore, after human acute monocytic leukemia cell line (THP-1) was stimulated with cagA-positive standard strains (cagA+ H. pylori 26695), the expression of periodontitis-related molecules Wnt5a, interleukin 8 (IL-8), interleukin 6 (IL-6) and interferon gamma (IFN-γ) significantly increased (p < 0.05). Conversely, the expression of tumor necrosis factor alpha (TNF-α) was almost stable. Meanwhile, cagA+ H. pylori promoted significantly higher expression of IL-8 and Wnt5a than isogenic cagA mutants strains (cagA− H. pylori 26695) did. Taken together, our data suggested that H. pylori might promote the growth of some periodontal pathogens and aggravate the progress of chronic periodontitis.

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Section: Discussionsupporting

confidence: 88%

Effect ofHelicobacter pyloriinfection on chronic periodontitis by the change of microecology and inflammation

Zhang

et al. 2016

Oncotarget

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“…More recently, Caliskan et al [ 40 ] performed a study to determine the relationship between among H. pylori genotypes and their effects on cytokine release levels. These authors studied five genotypes: cag A and vac A s1m2 (genotype 1); cag A and vac A s1m1 (genotype 2); cag A, vac A s1m2, and bab A2 (genotype 3); vac A s2m2 (genotype 4); and cag A and vac A s2m2 (genotype 5).…”

Section: Discussionmentioning

confidence: 99%

Lack of association among TNF-α gene expression, -308 polymorphism (G > A) and virulence markers of Helicobacter pylori

Zabaglia¹,

Ferraz²,

Pereira³

et al. 2015

J Venom Anim Toxins Incl Trop Dis

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BackgroundTumor necrosis factor plays a critical role in the pathogenesis of gastric diseases such as gastric cancer, and an abnormal inflammatory response has frequently been observed in dyspeptic patients. Helicobacter pylori infection can induce a gastric mucosal inflammatory response that may be influenced by -308 (G > A) polymorphisms and gene expression of the TNF-α gene.MethodsOne hundred and thirty-four gastric biopsy samples were collected from patients of both genders (61♂ and 73♀, mean age 40.3 ± 24.2 years) with gastric symptoms. The -308 (G > A) polymorphism of TNF-α was characterized using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). The expression level was measured using real-time PCR, and relative quantification (RQ) was calculated using the comparative CT method (2-ΔΔCT).ResultsThe analysis revealed an increase in TNF-α gene expression in patients with gastritis; on the other hand, no statistical differences were observed in patients with gastric cancer. In addition, no association was found among -308 polymorphism genotypes, virulence markers, or TNF-α gene expression.ConclusionsHelicobacter pylori induces a large increase in TNF-α expression in patients with gastritis, regardless of tissue inflammation, but after the tissue becomes neoplastic, the presence of bacteria did not influence expression. These results suggest that the TNF-α pathway may play an important role in the progression from gastritis to gastric cancer

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“…The pellet was resuspended in 10 ml complete RPMI-1640 medium and counted on the Thoma cell counting chamber by Trypan blue staining (1:10). Cell density was adjusted to 4 × 10 5 viable cells in the complete medium 26 .…”

Section: Methodsmentioning

confidence: 99%

Studying dynamic stress effects on the behaviour of THP-1 cells by microfluidic channels

Birol

Fucucuoglu

Çadırcı

et al. 2021

Sci Rep

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Atherosclerosis is a long-term disease process of the vascular system that is characterized by the formation of atherosclerotic plaques, which are inflammatory regions on medium and large-sized arteries. There are many factors contributing to plaque formation, such as changes in shear stress levels, rupture of endothelial cells, accumulation of lipids, and recruitment of leukocytes. Shear stress is one of the main factors that regulates the homeostasis of the circulatory system; therefore, sudden and chronic changes in shear stress may cause severe pathological conditions. In this study, microfluidic channels with cavitations were designed to mimic the shape of the atherosclerotic blood vessel, where the shear stress and pressure difference depend on design of the microchannels. Changes in the inflammatory-related molecules ICAM-1 and IL-8 were investigated in THP-1 cells in response to applied shear stresses in an continuous cycling system through microfluidic channels with periodic cavitations. ICAM-1 mRNA expression and IL-8 release were analyzed by qRT-PCR and ELISA, respectively. Additionally, the adhesion behavior of sheared THP-1 cells to endothelial cells was examined by fluorescence microscopy. The results showed that 15 Pa shear stress significantly increases expression of ICAM-1 gene and IL-8 release in THP-1 cells, whereas it decreases the adhesion between THP-1 cells and endothelial cells.

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The cytokine response in THP-1 (monocyte) and HL-60 (neutrophil-differentiated) cells infected with different genotypes of Helicobacter pylori strains

Cited by 11 publications

References 45 publications

Effect ofHelicobacter pyloriinfection on chronic periodontitis by the change of microecology and inflammation

Effect ofHelicobacter pyloriinfection on chronic periodontitis by the change of microecology and inflammation

Lack of association among TNF-α gene expression, -308 polymorphism (G > A) and virulence markers of Helicobacter pylori

Studying dynamic stress effects on the behaviour of THP-1 cells by microfluidic channels

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