The cytokinesis-block micronucleus assay as a biological dosimeter in spleen and peripheral blood lymphocytes of the mouse following acute whole-body irradiation

Cytokinesis-block micronucleus cytome assay

“…Lymphocytes are isolated either from the spleen or peripheral blood and cultured according to the procedures described by Fenech et al 92 . Because murine lymphocytes have shorter cell division cycles than human lymphocytes, it is essential to add Cyt-B no later than 18 h after stimulation by mitogen and to harvest the cells 20-30 h later.…”

Analysis of the expression of toll-like receptors 2 and 4 and cytokine production during experimental Leishmania chagasi infection

“…Macrophage culture -After spleen removal, splenic macrophages were obtained according to protocol described in Fenech et al (1991). Briefly, spleen fragments were removed and placed into Petri dishes (20 x 100 mm) containing 5 mL of Roswell Park Memorial Institute medium (RPMI) 1640 culture medium (Sigma-Aldrich, St. Louis, MO, USA).…”

The Cytokinesis-Block Micronucleus Technique and Its Application to Genotoxicity Studies in Human Populations

“…By contrast, the MN frequency in peripheral blood lymphocytes increased during the week after irradiation, but ultimately MN frequencies in the blood and spleen became approximately the same by day 14 and did not differ significantly from the MN frequency observed in blood lymphocytes immediately after exposure (27). Such data suggest that reliable dose estimates with the CBMN assay can still be obtained up to 2 weeks after whole body irradiation.…”

“…As a result of the possible application of the CBMN assay in biological dosimetry after radiation accidents, several laboratories around the world have now established the dose-response parameters after exposure to various sources of ionizing radiation (3,4,6,7,(20)(21)(22)(23)(24)(25)29). Furthermore, the Sobel's parallelogram concept of risk estimation (26) is also being implemented to determine the kinetics and extent of MN expression using the mouse as a model (27,28).…”