The Cytological Localization of Erythropoietin in the Human Kidney Using the Fluorescent Antibody Technique

Busuttil, Ronald W.; Roh, Byung L.; Fisher, James W.

doi:10.3181/00379727-137-35570

Cited by 33 publications

(11 citation statements)

References 7 publications

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“…The specific yellow-green fluorescent staining was only seen in the glo merular tufts and was the most intense in the periphery of the glomerulus. This pattern was quite similar to that described for the anemic human kidney [6]. Hypoxic dog kidney sections treated with either normal rab bit serum or with antiserum which had been previously neutralized with highly purified human urinary ESF did not show any fluorescence.…”

Section: Methodssupporting

confidence: 64%

“…Our previous report [6] of localization of ESF in the epithelial cell of the glomerular tuft using the fluorescent antibody technique was not completely definitive because it was possible that the epithelial cell could have trapped ESF as blood with high levels of ESF circulated through the kidney. The results of the present study indicate that when plasma levels of ESF are elevated without stimulating kidney production of ESF, the glomerulus does not show positive fluorescence with the use of the fluorescent ESF-antibody.…”

Section: Discussionmentioning

confidence: 99%

“…[4] and F renkel et al [5] have localized ESF in the glomerular tuft of anemic sheep kidney. B usuttil et al [6] have also re ported a cytological localization of ESF in the epithelial cells of the glomerular tuft in an anemic human kidney using the fluorescent anti body technique. The cytoplasmic localization in the epithelial cells of the glomerulus was confirmed by destaining the fluorescein labelled tissue section and restaining for morphological study with light microscopy.…”

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confidence: 99%

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Localization of Erythropoietin in the Glomerulus of the Hypoxic Dog Kidney Using a Fluorescent Antibody Technique

Busuttil¹,

Roh²,

Fisher³

1972

Acta Haematol

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Intense fluorescent staining of cells in the glomerular tuft has been demonstrated in a hypoxic dog kidney with an antibody to erythropoietin (ESF) using the indirect fluorescent antibody technique. The fluorescent staining in the glomeruli was blocked by a highly purified preparation of ESF. This fluorescence appears to be localized in the visceral epithelial cells of the giomerulus. Kidney sections of a normal dog and a dog injected with hypoxic dog plasma ESF to maintain a prolonged elevation in plasma ESF titers failed to show positive fluorescent staining following treatment with the antibody to ESF. The present studies suggest that the epithelial cells of the glomerular tuft are the site of ESF production.

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Section: Methodssupporting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Localization of Erythropoietin in the Glomerulus of the Hypoxic Dog Kidney Using a Fluorescent Antibody Technique

Busuttil¹,

Roh²,

Fisher³

1972

Acta Haematol

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“…Those studies localized EPO in the glomerular tuft of an anemic-sheep kidney (Fisher et al 1965 ;Frenkel et al 1968). Busuttil et al (1971Busuttil et al ( , 1972 reported, by the same method that the epithelial cell of the glomerular tuft in both the human and hypoxic dog kidney was the most likely source of EPO. The anti-EPO sera used by the latter researchers were anti-crude human EPO rabbit serum and those used by the former researchers were anti-partially purified sheep EPO rabbit serum.…”

Section: )mentioning

confidence: 99%

Localization of renal erythropoietin and the role of Ia antigen expression in hypoxic rat glomeruli.

Fukushima

Yanagisawa

Nakamoto

et al. 1990

Tohoku J. Exp. Med.

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Erythropoietin (EPO) producing cells were examined by the immunofluorescent antibody technique, and the alterations in the Ia antigen expression in glomeruli by hypoxic stimuli. Antisera were obtained from rabbits immunized with pure human EPO. Positive fluorescence was localized in glomerular mesangial regions of the hypoxic rat kidney. Although hypoxia showed neither increase in the number of glomerular cells nor hypertrophy of the mesangial matrix, it induced an increase of the Ia antigen expressions in glomeruli. The peak of the increase occurred 2 hr after hypoxia and then decreased until 24 hr later. The increase of Ia antigen after 2 hr of hypoxic stimuli was 176.5 + 7.0% of normal controls, and ranged from 151 to 209%. In conclusion, Ia positive cells in glomeruli may have some relationship to the stimulation of EPO production. erythropoietin (EPO) ; Ia antigen ; hypoxia Erythropoietin (EPO) is the glycoprotein hormone which is the primary factor regulating erythropoiesis, and is produced by the kidney. Although the molecular structure of EPO has been described earlier (Jacobs et al. 1985 ;Lin et al. 1985), the producing cells or sites have remained unclear. There are many reports about the localization of EPO production within the kidney. The cells responsible for EPO production are most probably localized in the kidney cortex. Significantly more EPO was extracted from the cortex than from the medulla.

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“…Using the fluorescent antibody technique, Busuttil et al ( 1 ) suggested that the production or storage of EP was probably localised in the cells of the glomerular epithelium, while the juxta-glomerular apparatus contained no EP. Using the fluorescent antibody technique, Busuttil et al ( 1 ) suggested that the production or storage of EP was probably localised in the cells of the glomerular epithelium, while the juxta-glomerular apparatus contained no EP.…”

Section: Introductionmentioning

confidence: 99%

Activity of Erythropoietin and Renin in Renal Venous Blood of Hypertensive Patients

Takács

Kim

Vajda

1976

Eur J Clin Investigation

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Plasma activities of renin and erythropoietin were determined in the renal veins of the right and left kidneys of hypertensive patients. The patients were divided into the following groups either according to the origin of the hypertension (group 1: control for essential hypertension, group 2: renovascular hypertension) or according to the hormone levels (group 3: renin activity exceeding 10 ng/litre in at least one of the renal veins and group 4: erythropoietin activity higher than 4% 59Fe incorporation in at least one of the renal veins). In groups 1, 2 and 3 a statistically significant difference in renin activity was found between the kidney with the higher renin activity and that with the lower activity. However, in group 4, the side, which showed elevated erythropoietin values also had higher renin activity as compared to the essential hypertensive group. Erythropoietin activity probably does not parallel the increased renin activity found in renovascular hypertension or in some cases of non-renovascular hypertension. However, in several cases of renal vascular alterations, both systems can be activated simultaneously.

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The Cytological Localization of Erythropoietin in the Human Kidney Using the Fluorescent Antibody Technique

Cited by 33 publications

References 7 publications

Localization of Erythropoietin in the Glomerulus of the Hypoxic Dog Kidney Using a Fluorescent Antibody Technique

Localization of Erythropoietin in the Glomerulus of the Hypoxic Dog Kidney Using a Fluorescent Antibody Technique

Localization of renal erythropoietin and the role of Ia antigen expression in hypoxic rat glomeruli.

Activity of Erythropoietin and Renin in Renal Venous Blood of Hypertensive Patients

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