The cytology of the vitellogenic stages of oogenesis in Drosophila melanogaster

Cummings, Michael R.; Brown, Nancy Marie; King, Robert C.

doi:10.1007/bf00324615

Cited by 49 publications

(25 citation statements)

References 10 publications

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“…In the present study it can not be established whether they also contribute to the formation of the vitelline membrane. However, electron microscopic studies e.g., those by King and Koch (1963) and Cummings (1968) have shown at least in certain dipterans the association of follicle cells with the formation of the vitelline membrane. The compound yolk develops from the yolk precursors infiltrating through intercellular spaces in the follicular epithelium during stage 12a of the oocyte.…”

Section: Resultsmentioning

confidence: 99%

Cytochemical studies on growth and differentiation of oocyte and follicular epithelium in relation to the development of yolk in Acrida turrita(insecta: Orthoptera)

et al. 1982

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Section: Resultsmentioning

confidence: 99%

Cytochemical studies on growth and differentiation of oocyte and follicular epithelium in relation to the development of yolk in Acrida turrita(insecta: Orthoptera)

et al. 1982

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“…Cummings et al [20) noted that the formation of vitelline membrane and chorion was an interaction between the follicular epithelium and the ooplasm, and that the follicular epithelium alone could not form these membranes. The size gradation of the nurse cells' nuclei, with the large one adjacent to the oocyte and small ones at the apical end of the follicle, is evident in L. exigua.…”

Section: Resultsmentioning

confidence: 99%

Cytology and histochemistry of the ovary in the buffalo-fly,Lyperosia exigua(De Meijere) (Diptera: Muscidae)

Khurad¹,

Thakare

1980

International Journal of Invertebrate Reproduction

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297ioles. The follicle consists_ <;>fan o~cyte, 15 nurs_e cells a~d an envelope o! follicular epitheioles. The follicle consists of an oocyte, 15 nurse cell and an envelope of follicular epithelium. The oocyte and the nurse cells are interconnected by a system of ring canals. During vitellogenesis, the oocyte shows changes in its shape, size and cytological organization. The process of egg maturation has been divided into a series of 10 consecutive stages. PAS-positive material appeared first in the oocyte periphery during stage 9, and later on migrated towards the center. The nurse and follicular epithelial cells were PAS-negative. DNA was abundant in the nuclei of both the nurse and follicular epithelial cells during stages 3-7 but was not detectable with Feulgen's test in the oocyte nucleus at any stage. RNA was present in the ooplasm during stages 6-8 but in subsequent stages gradually decreased. During stages 4-7, high concentration of RNA was found in the follicular epithelium. The protein yolk appeared first towards the periphery of the oocyte adjacent to the follicular epithelium during stages 5 and 6, and then migrated towards the center of the oocyte. The sudanophilic lipid droplets, first seen in the nurse cells during stage 5, increased in numbers during stages 6 and 7. These droplets subsequently migrated from nurse cells to the oocyte via ring canal.

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“…One should also be careful to avoid the analysis of damaged ovarioles ( see Ref. 20) or those where immunostaining did not work well. The most common types of analyses performed in our lab are described below, starting with germline analyses involving ovarioles followed by those focused on the germarium, and ending with analyses of the follicle cell lineage.…”

Section: Methodsmentioning

confidence: 99%

“…Vitellogenesis begins at stage 8 of oogenesis [21]. To assess the progression of mutant cysts through vitellogenesis, we quantify the fraction of ovarioles that contain a GFP-negative vitellogenic cyst in control versus mutant mosaic ovarioles (Fig.…”

Section: Methodsmentioning

confidence: 99%

Genetic Mosaic Analysis of Stem Cell Lineages in the Drosophila Ovary

Laws

Drummond‐Barbosa

2015

Methods in Molecular Biology

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Genetic mosaic analyses represent an invaluable approach for the study of stem cell lineages in the Drosophila ovary. The generation of readily identifiable, homozygous mutant cells in the context of wild-type ovarian tissues within intact organisms allows the pinpointing of cellular requirements for gene function, which is particularly important for understanding the physiological control of stem cells and their progeny. Here, we provide a step-by-step guide to the generation and analysis of genetically mosaic ovaries using flippase (FLP)/FLP recognition target (FRT)-mediated recombination in adult Drosophila melanogaster, with a focus on processes of oogenesis that are controlled by diet-dependent factors.

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The cytology of the vitellogenic stages of oogenesis in Drosophila melanogaster

Cited by 49 publications

References 10 publications

Cytochemical studies on growth and differentiation of oocyte and follicular epithelium in relation to the development of yolk in Acrida turrita(insecta: Orthoptera)

Cytochemical studies on growth and differentiation of oocyte and follicular epithelium in relation to the development of yolk in Acrida turrita(insecta: Orthoptera)

Cytology and histochemistry of the ovary in the buffalo-fly,Lyperosia exigua(De Meijere) (Diptera: Muscidae)

Genetic Mosaic Analysis of Stem Cell Lineages in the Drosophila Ovary

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