The cytostome-cytopharynx complex of<i>Trypanosoma cruzi</i>epimastigotes disassembles during cell division

Alcantara, Carolina de Lima; Vidal, Juliana C.; Souza, Wanderley de; Cunha-e-Silva, Narcisa L.

doi:10.1242/jcs.187419

Cited by 26 publications

(30 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Through the cell cycle, these two structures have similar dynamics, extending toward the cell posterior and then disassembling before cell division. This pattern of cell cycle dynamics is similar to that of the cytostome/cytopharynx and its associated microtubules in T. cruzi (Alcantara et al, ). We differentiated the promastigote cells expressing CPA::mNG and mCh::SPEF1 in vitro to axenic amastigotes and then imaged them (Figure A1).…”

Section: Resultssupporting

confidence: 77%

“…The dynamic nature of the MVT lysosome has striking parallels with cytostome/cytopharynx of T. cruzi , which also undergoes a similar cell cycle regulation, disassembling before cytokinesis, and reassembling afterward (Alcantara et al, ). Moreover, the MVT lysosome and the cytostome/cytopharynx have a similar membrane tubule structure with associated microtubules which nucleate near the flagellar pocket.…”

Section: Discussionmentioning

confidence: 99%

“…Conversely, the microtubule triplet is nucleated near the cytostome/cytopharynx entrance, and together, these two sets of microtubules form a V shape upon which the cytostome/cytopharynx sits (Alcantara et al, ). In the latter stages of the cell cycle, during G 2 prior to flagellar pocket division, the cytostome/cytopharynx complex and associated microtubules are disassembled, and then, the structure reassembles during late cytokinesis (Alcantara, L., Vidal, J.C., Souza, W. de, & Cunha‐e‐Silva, N.L., ). Interestingly, it has also been shown that the MVT lysosome in dividing Leishmania cells also disassembles forming one or two sets of vesicles (Ilgoutz et al, ; Weise et al, ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Lysosome assembly and disassembly changes endocytosis rate through the Leishmania cell cycle

2019

View full text Add to dashboard Cite

The Leishmania lysosome has an atypical structure, consisting of an elongated vesicle-filled tubule running along the anterior-posterior axis of the cell, which is termed the multivesicular tubule (MVT) lysosome. Alongside, the MVT lysosome is one or more microtubules, the lysosomal microtubule(s). Previous work indicated there were cell cycle-related changes in MVT lysosome organization; however, these only provided snapshots and did not connect the changes in the lysosomal microtubule(s) or lysosomal function. Using mNeonGreen tagged cysteine peptidase A and SPEF1 as markers of the MVT lysosome and lysosomal microtubule(s), we examined the dynamics of these structures through the cell cycle. Both the MVT lysosome and lysosomal microtubule(s) elongated at the beginning of the cell cycle before plateauing and then disassembling in late G 2 before cytokinesis. Moreover, the endocytic rate in cells where the MVT lysosome and lysosomal microtubule(s) had disassembled was extremely low. The dynamic nature of the MVT lysosome and lysosomal microtubule(s) parallels that of the Trypanosoma cruzi cytostome/cytopharynx, which also has a similar membrane tubule structure with associated microtubules. As the cytostome/cytopharynx is an ancestral feature of the kinetoplastids, this suggests that the Leishmania MVT lysosome and lysosomal microtubule(s) are a reduced cytostome/cytopharynx-like feature.

show abstract

Section: Resultssupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lysosome assembly and disassembly changes endocytosis rate through the Leishmania cell cycle

2019

View full text Add to dashboard Cite

show abstract

“…Smaller flagella were also observed ( Figure 3C). Surprisingly, parasites in final stages of cell division (just before cytokinesis, when cells are connected by their posterior end with one flagellum in each anterior end (Alcantara et al, 2017) were observed with another new flagellum already in each anterior end (Supplementary Figure 1), thus reinforcing the idea that these cells somehow lost at least part of their cell cycle coordination.…”

Section: Knockout Of Rbp Genesmentioning

confidence: 66%

RNA Binding Proteins and Gene Expression Regulation in Trypanosoma cruzi

Romagnoli

Holetz

Alves

et al. 2020

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

The regulation of gene expression in trypanosomatids occurs mainly at the post-transcriptional level. In the case of Trypanosoma cruzi, the characterization of messenger ribonucleoprotein (mRNP) particles has allowed the identification of several classes of RNA binding proteins (RBPs), as well as non-canonical RBPs, associated with mRNA molecules. The protein composition of the mRNPs as well as the localization and functionality of the mRNAs depend on their associated proteins. mRNPs can also be organized into larger complexes forming RNA granules, which function as stress granules or P-bodies depending on the associated proteins. The fate of mRNAs in the cell, and consequently the genes expressed, depends on the set of proteins associated with the messenger molecule. These proteins allow the coordinated expression of mRNAs encoding proteins that are related in function, resulting in the formation of post-transcriptional operons. However, the puzzle posed by the combinatorial association of sets of RBPs with mRNAs and how this relates to the expressed genes remain to be elucidated. One important tool in this endeavor is the use of the CRISPR/CAS system to delete genes encoding RBPs, allowing the evaluation of their effect on the formation of mRNP complexes and associated mRNAs in the different compartments of the translation machinery. Accordingly, we recently established this methodology for T. cruzi and deleted the genes encoding RBPs containing zinc finger domains. In this manuscript, we will discuss the data obtained and the potential of the CRISPR/CAS methodology to unveil the role of RBPs in T. cruzi gene expression regulation.

show abstract

“…This complex is formed de novo from the flagellar pocket membrane during cytokinesis. This experiment elucidated the biogenesis of this structure [40] (Figure 3B1-B3). The same methodology was used to obtain 3D reconstructions of the ultrastructural changes present in the intermediate forms of T. cruzi during the metacyclogenesis process (differentiation of epimastigotes into metacyclic trypomastigotes).…”

Section: Microtubulesmentioning

confidence: 78%

Morphological and Functional Aspects of Cytoskeleton of Trypanosomatids

Vidal¹,

Souza²

2017

Cytoskeleton - Structure, Dynamics, Function and Disease

View full text Add to dashboard Cite

Trypanosomatidae are protozoans that include monogenetic parasites, such as the Blastocrithidia and Herpetomonas genera, as well as digenetic parasites, such as the Tr yp an os oma and Leishmania genera. Their life cycles alternate between insect vectors and mammalian hosts. The parasite's life cycle involves symmetrical division and different transitional developmental stages. In trypanosomatids, the cytoskeleton is composed of subpellicular microtubules organized in a highly ordered array of stable microtubules located beneath the plasma membrane, the paraflagellar rod, which is a lattice-like structure attached alongside the flagellar axoneme and a cytostome-cytopharynx. The complex life cycle, the extremely precise cytoskeletal organization and the single copy structures present in trypanosomatids provide interesting models for cell biology studies. The introduction of molecular biology, FIB/SEM (focused ion beam scanning electron microscopy) and electron microscopy tomography approaches and classical methods, such as negative staining, chemical fixation and ultrafast cryofixation have led to the determination of the three-dimensional (3D) structural organization of the cells. In this chapter, we highlight the recent findings on Trypanosomatidae cytoskeleton emphasizing their structural organization and the functional role of proteins involved in the biogenesis and duplication of cytoskeletal structures. The principal finding of this review is that all approaches listed above enhance our knowledge of trypanosomatids biology showing that cytoskeleton elements are essential to several important events throughout the protozoan life cycle.

show abstract

The cytostome-cytopharynx complex ofTrypanosoma cruziepimastigotes disassembles during cell division

Cited by 26 publications

References 35 publications

Lysosome assembly and disassembly changes endocytosis rate through the Leishmania cell cycle

Lysosome assembly and disassembly changes endocytosis rate through the Leishmania cell cycle

RNA Binding Proteins and Gene Expression Regulation in Trypanosoma cruzi

Morphological and Functional Aspects of Cytoskeleton of Trypanosomatids

Contact Info

Product

Resources

About