The Cytotoxic Effect of Denture Base Polymers

Hensten‐Pettersen, Arne; Wictorin, L

doi:10.3109/00016358109162267

Cited by 41 publications

(19 citation statements)

References 9 publications

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“…First, the degree of polymerization affects the mechanical and geometric properties of resultant prostheses 29–31 . Second, unreacted monomer may produce undesirable effects in the human body 32–34 . Consequently, resins displaying greater degrees of polymerization may provide substantial clinical advantages.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Mechanical and Thermal Properties of Commonly Used Denture Base Resins

Phoenix

Mansueto

Ackerman³

et al. 2004

Journal of Prosthodontics

115

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Section: Discussionmentioning

confidence: 99%

Evaluation of Mechanical and Thermal Properties of Commonly Used Denture Base Resins

Phoenix

Mansueto

Ackerman³

et al. 2004

Journal of Prosthodontics

115

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show abstract

“…Legal regulations for dental laboratories are based on a directive regarding hazardous substances and the technical rules for hazardous materials that is TRGS 900 51. It was reported that MMA vapor in dental practices caused vertigo 52, 53. There is no evidence, however, that serious problems may be caused by inhaling PMMA ingredients, although MMA may irritate the eyes, skin, and respiratory system.…”

Section: Systemic Toxicitymentioning

confidence: 99%

“…The cellular compatibility of solid specimens, aqueous resin extracts, formaldehyde, and MMA were investigated in permanent cells and primary cultures as well 44–46. A study of Hensten‐Pettersen and Wictorin53 revealed clear toxic reactions caused by solid specimens of two orthodontic acrylic resins (one autopolymerizing and another light‐curable) in permanent cultures of fibroblasts and keratinocytes. The cytotoxic effect was greater in autopolymerized resins.…”

Section: Local Toxicity and Tissue Compatibilitymentioning

confidence: 99%

Biocompatibility of polymethylmethacrylate resins used in dentistry

et al. 2012

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Biocompatibility or tissue compatibility describes the ability of a material to perform with an appropriate host response when applied as intended. Poly-methylmethacrylate (PMMA) based resins are most widely used resins in dentistry, especially in fabrication of dentures and orthodontic appliances. They are considered cytotoxic on account of leaching of various potential toxic substances, most common being residual monomer. Various in vitro and in vivo experiments and cell based studies conducted on acrylic based resins or their leached components have shown them to have cytotoxic effects. They can cause mucosal irritation and tissue sensitization. These studies are not only important to evaluate the long term clinical effect of these materials, but also help in further development of alternate resins. This article reviews information from scientific full articles, reviews, or abstracts published in dental literature, associated with biocompatibility of PMMA resins and it is leached out components. Published materials were searched in dental literature using general and specialist databases, like the PubMED database.

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“…They may cause mucosal irritation and sensitization of the oral tissues. [3][4][5][6][7][8][9] Clinical symptoms include burning sensation in the mouth, redness, vesicles, ulceration, swelling and edema. 10 Previous studies have confirmed that resin materials are potentially toxic to various degrees based on their specific composition, 11 porosity, type of polymerization reaction, 12,13 technique used for polymerization 14 and post polymerization material handling.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Effects of Three Denture Base Materials on Gingival Epithelial Cells and Fibroblasts: An in vitro Study

Wicks¹,

Babu²,

Garcı́a-Godoy³

et al. 2015

International Journal of Experimental Dental Science

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Objectives: Modern polyamide 'flexible' denture base materials have increased in popularity for use in removable partial dentures. The introduction of these new products warrants investigation of their relative potential for toxicity. The purpose of this study was to investigate three contemporary denture base materials used in fabricating definitive prosthetic restorations. Materials and methods:Two 'flexible' materials (Valplast™ and Duraflex™) formed by thermoplastic injection molding technique, and one traditional heat processed, methyl methacrylate resin material (Lucitone 199) were evaluated. Cultured gingival epithelial cells and fibroblasts were treated with conditioned media prepared from denture material disks and then assayed for cell toxicity by [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) cell viability assay. Cell membrane damage was determined by measuring the release of cytoplasmic lactate dehydrogenase. Further confirmation of toxicity induced by the conditioned media was determined by staining the cells with live/dead stain and observing under a UV microscope.Results: Data were analyzed by means of a linear model ANOVA followed by Tukey's post hoc tests for comparison among groups. The significance level adopted was 5% (p < 0.05). The three denture materials differed in their toxicity to the cells as assessed by MTT assay. Valplast conditioned media in general, especially the media of unpolished disks, was found to be toxic to both gingival fibroblasts and epithelial cells while media obtained from polished Lucitone and Duraflex were found to be less toxic. After 7 days of incubation with Valplast unpolished conditioned media, only 1 to 2% of the cells remained viable, while the polished disk conditioned media caused significantly less (p < 0.05) toxicity, approximately 76 and 92% of fibroblasts and epithelial cells respectively, were viable. After 7 days of incubation with media obtained from the other denture materials, 35 to 92% of fibroblasts and epithelial cells were found to be viable. The data obtained from lactate dehydrogenase (LDH) assay and live/dead mammalian cell viability assay were in agreement with the MTT viability assay. Conclusion:Conditioned media from unpolished Valplast denture material appeared to be significantly more toxic to gingival fibroblasts and epithelial cells when compared to the polished Lucitone disk conditioned media as well as the media obtained from Duraflex.

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The Cytotoxic Effect of Denture Base Polymers

Cited by 41 publications

References 9 publications

Evaluation of Mechanical and Thermal Properties of Commonly Used Denture Base Resins

Evaluation of Mechanical and Thermal Properties of Commonly Used Denture Base Resins

Biocompatibility of polymethylmethacrylate resins used in dentistry

Cytotoxic Effects of Three Denture Base Materials on Gingival Epithelial Cells and Fibroblasts: An in vitro Study

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