The DASH complex and Klp5/Klp6 kinesin coordinate bipolar chromosome attachment in fission yeast

Sanchez-Perez, Isabel; Renwick, Steven John; Crawley, Karen; Karig, Inga; Buck, Vicky; Meadows, John C.; Franco-Sanchez, Alejandro; Fleig, Ursula; Toda, Takashi; Millar, Jonathan

doi:10.1038/sj.emboj.7600761

Cited by 126 publications

(164 citation statements)

References 50 publications

Supporting

Mentioning

147

Contrasting

Unclassified

Order By: Relevance

“…By using strains that have a single Dam1 subunit tagged with GFP and expressed from the endogenous promoter (here, called mono-GFP-tagged Dam1), SpDam1 complex was previously detected to localize to kinetochores, the spindle, and intranuclear MTs in mitotic cells (20)(21)(22). In addition to these sites, by careful microscopic examination, we also detected weak, discrete spots of Dam1 assemblies on the interphase cytoplasmic MTs (Fig.…”

Section: Identification Of Discrete Dam1 Assemblies On Cytoplasmic Mtmentioning

confidence: 87%

See 1 more Smart Citation

A non-ring-like form of the Dam1 complex modulates microtubule dynamics in fission yeast

Gao

Courthéoux

Gachet

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The Dam1 complex is a kinetochore component that couples chromosomes to the dynamic ends of kinetochore microtubules (kMTs). Work in the budding yeast Saccharomyces cerevisiae has shown that the Dam1 complex forms a 16-unit ring encircling and tracking the tip of a MT in vitro, consistent with its cellular function as a coupler. Dam1 also forms smaller, nonring patches in vitro that track the dynamic ends of MTs. However, the identity of Dam1’s functional form in vivo remains unknown. Here we report a comprehensive in vivo characterization of Dam1 in the fission yeast Schizosaccharomyces pombe . In addition to their dense localizations on kinetochores and spindle MTs during mitosis, we identify that Dam1 is also localized onto cytoplasmic MTs as discrete spots in interphase, providing the unique opportunity to analyze Dam1 oligomers at the single-particle resolution in live cells. Such analysis shows that each oligomer contains one to five copies of Dam1, and is able to “switch-rail” while moving along MTs, precluding the possibility of a 16-unit encircling structure. Dam1 patches track the plus ends of the shortening, but not the elongating, MTs and retard MT depolymerization. Together with Mal3, the EB1-like MT-interacting protein, cytoplasmic Dam1 plays an important role in maintaining proper cell shape. In mitosis, kinetochore-associated Dam1 appears to facilitate kMT depolymerization. Together, our findings suggest that patches, instead of rings, are the physiologically functional forms of Dam1 in pombe. Our findings help establish the benchmark parameters of the Dam1 coupler and elucidate the mechanism of its functions.

show abstract

Section: Identification Of Discrete Dam1 Assemblies On Cytoplasmic Mtmentioning

confidence: 87%

“…20). Similar to ScDam1, SpDam1 is localized at kinetochores as well as on the spindle and the intranuclear MT (20)(21)(22), and functions in coupling kinetochores to MTs (22)(23)(24). Using quantitative fluorescence microscopy, it was determined that on average only seven to eight copies of SpDam1 per kinetochore were found (25).…”

mentioning

confidence: 99%

A non-ring-like form of the Dam1 complex modulates microtubule dynamics in fission yeast

Gao

Courthéoux

Gachet

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Ten S. pombe proteins, which are conserved in the S. cerevisiae DASH complex, were purified as a complex by biochemical purification . The DASH complex is localized at the kinetochore and the spindle at mitotic phase and functions with Klp5/6 to capture the kinetochore (Sanchez-Perez et al, 2005), indicating that it plays a role in spindle attachment during chromosome segregation. In addition to these mitotic centromere proteins, meiosis-specific centromere proteins, Sgo1 and Moa1, that play an important role in meiotic chromosome segregation have been characterized in S. pombe.…”

Section: Introductionmentioning

confidence: 99%

Reconstruction of the Kinetochore during Meiosis in Fission Yeast Schizosaccharomyces pombe

Hayashi

Asakawa

Haraguchi

et al. 2006

MBoC

View full text Add to dashboard Cite

During the transition from mitosis to meiosis, the kinetochore undergoes significant reorganization, switching from a bipolar to a monopolar orientation. To examine the centromere proteins that are involved in fundamental reorganization in meiosis, we observed the localization of 22 mitotic and 2 meiotic protein components of the kinetochore during meiosis in living cells of the fission yeast. We found that the 22 mitotic proteins can be classified into three groups: the Mis6-like group, the NMS (Ndc80-Mis12-Spc7) group, and the DASH group, based on their meiotic behavior. Mis6-like group proteins remain at the centromere throughout meiosis. NMS group proteins disappear from the centromere at the onset of meiosis and reappear at the centromere in two steps in late prophase. DASH group proteins appear shortly before metaphase of meiosis I. These observations suggest that Mis6-like group proteins constitute the structural basis of the centromere and that the NMS and DASH group proteins reassemble to establish the functional metaphase kinetochore. On the other hand, the meiosis-specific protein Moa1, which plays an important role in forming the meiotic monopolar kinetochore, is loaded onto the centromere significantly earlier than the NMS group, whereas another meiosis-specific protein, Sgo1, is loaded at times similar to the NMS group.

show abstract

“…However, while such rings may efficiently couple microtubule depolymerization to chromosome movement in a system where kinetochores bind only to a single microtubule [130,131], they are not essential in fission yeast [132] and orthologues have never been found outside fungi. Additionally, it was subsequently shown that ring formation is not required for microtubule depolymerization-driven motion of the Dam1 complex [133,134].…”

Section: Force Generation By Microtubule Depolymerization From Plus-endsmentioning

confidence: 99%

The perpetual movements of anaphase

Maiato

Lince-Faria

2010

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

One of the most extraordinary events in the lifetime of a cell is the coordinated separation of sister chromatids during cell division. This is truly the essence of the entire mitotic process and the reason for the most profound morphological changes in cytoskeleton and nuclear organization that a cell may ever experience. It all occurs within a very short time window known as "anaphase", as if the cell had spent the rest of its existence getting ready for this moment in an ultimate act of survival. And there is a good reason for this: no space for mistakes. Problems in the distribution of chromosomes during cell division have been correlated with aneuploidy, a common feature observed in cancers and several birth defects, and the main cause of spontaneous abortion in humans. In this paper we critically review the mechanisms of anaphase chromosome motion that resisted the scrutiny of more than one hundred years of research as part of a tribute to the pioneering work of Miguel Mota.

show abstract

The DASH complex and Klp5/Klp6 kinesin coordinate bipolar chromosome attachment in fission yeast

Cited by 126 publications

References 50 publications

A non-ring-like form of the Dam1 complex modulates microtubule dynamics in fission yeast

A non-ring-like form of the Dam1 complex modulates microtubule dynamics in fission yeast

Reconstruction of the Kinetochore during Meiosis in Fission Yeast Schizosaccharomyces pombe

The perpetual movements of anaphase

Contact Info

Product

Resources

About