The dechlorination of TCE by a perchlorate reducing consortium

Wen, Li Lian; Zhang, Yin; Chen, Jia Xian; Zhang, Zhao Xin; Yi, Yong; Tang, Youneng; Rittmann, Bruce E.; Zhao, He

doi:10.1016/j.cej.2016.11.021

Cited by 35 publications

(18 citation statements)

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“…capable of complete dechlorination, provides a unique solution for remediation of contaminated sites. However, bioaugmentation process requires high cell density, upto 10 12 Dehalococcoides cells L −1 (Waller et al 2005;Duhamel and Edwards 2006;Holmes et al 2006;Hug et al 2012;Delgado et al 2014;Wen et al 2017;Saiyari et al 2018) in active cultures and the transformation of cis-DCE and VC, avoiding the accumulation of the nascent DCE and VC in the environment (Holmes et al 2006). To establish dechlorinating enrichments, microbial communities were collected and enriched from different anaerobic sites contaminated with PCE and TCE (Duhamel et al 2002;Holmes et al 2006), and moreover from sediment of river and tropical mangrove and garden soils not exposed to contamination (He et al 2005;Ziv-El et al 2011;Delgado et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of site-specific dechlorinating microbial inocula capable of complete dechlorination enriched in anaerobic microcosms amended with clay mineral

Nagymáté

Jurecska

Romsics

et al. 2020

World J Microbiol Biotechnol

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Short-chain halogenated aliphatic hydrocarbons (e.g. perchloroethene, trichloroethene) are among the most toxic environmental pollutants. Perchloroethene and trichloroethene can be dechlorinated to non-toxic ethene through reductive dechlorination by Dehalococcoides sp. Bioaugmentation, applying cultures containing organohalide-respiring microorganisms, is a possible technique to remediate sites contaminated with chlorinated ethenes. Application of site specific inocula is an efficient alternative solution. Our aim was to develop site specific dechlorinating microbial inocula by enriching microbial consortia from groundwater contaminated with trichloroethene using microcosm experiments containing clay mineral as solid phase. Our main goal was to develop fast and reliable method to produce large amount (100 L) of bioactive agent with anaerobic fermentation technology. Polyphasic approach has been applied to monitor the effectiveness of dechlorination during the transfer process from bench-scale (500 mL) to industrial-scale (100 L). Gas chromatography measurement and T-RFLP (Terminal Restriction Fragment Length Polymorphism) revealed that the serial subculture of the enrichments shortened the time-course of the complete dechlorination of trichloroethene to ethene and altered the composition of bacterial communities. Complete dechlorination was observed in enrichments with significant abundance of Dehalococcoides sp. cultivated at 8 °C. Consortia incubated in fermenters at 18 °C accelerated the conversion of TCE to ethene by 7-14 days. Members of the enrichments belong to the phyla Bacteroidetes, Chloroflexi, Proteobacteria and Firmicutes. According to the operational taxonomic units, main differences between the composition of the enrichment incubated at 8 °C and 18 °C occurred with relative abundance of acetogenic and fermentative species. In addition to the temperature, the site-specific origin of the microbial communities and the solid phase applied during the fermentation technique contributed to the development of a unique microbial composition.

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Section: Introductionmentioning

confidence: 99%

Preparation and characterization of site-specific dechlorinating microbial inocula capable of complete dechlorination enriched in anaerobic microcosms amended with clay mineral

Nagymáté

Jurecska

Romsics

et al. 2020

World J Microbiol Biotechnol

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“…Chlorinated ethenes (TCE, cis -DCE, VC), ethene, chlorinated ethanes (TCA, DCA, CA) and methane were measured by injecting 100 μL of headspace samples with a gas-tight syringe into a gas chromatograph (Agilent Technologies GC system, model 6890N, Agilent Technologies, Inc., United States) equipped with a flame-ionization detector (FID), and a packed column (30 m long, 0.32 mm i.d., 0.5 μm thickness, cross-linked polydimethysiloxane film, J&W Scientific, United States) ( Zhao H.P. et al, 2010 ; Ziv-El et al, 2011 ; Wen et al, 2017 ). N 2 was the carrier gas fed at a constant flow rate of 0.065 m 3 /d, and the temperature conditions for injector and detector were 200 and 250°C, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Microbially mediated anaerobic reductive dechlorination is a good strategy for the remediation of chlorinated compounds. For example, TCE can be converted to dichloroethene (DCE), vinyl chloride (VC), and finally non-toxic ethene in a stepwise manner ( Lee et al, 2013 ; Wen et al, 2017 ) by various microorganisms. Dehalococcoides (which belong to Chloroflexi) are the only known bacteria that completely transform TCE all way down to ethene, although many other microorganisms have been found to partially reduce TCE to DCE or VC, including Geobacter, Desulfovibrio, Desulfuromonas (which belong to Proteobacteria), Dehalobacter and Desulfitobacterium (which belong to Firmicutes), and Dehalobium , and Dehalogenimonas (which belong to Chloroflexi) ( Duhamel and Edwards, 2006 ; Maphosa et al, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

“…Dechlorinating microorganisms are always in a mixed culture, which contains many other syntrophic microbes, e.g., fermenters, methanogens and acetogens, that contribute to the rigorous nutrient requirements of the dechlorinators ( He et al, 2003 ; Kittelmann and Friedrich, 2008 ; Men et al, 2013 ; Wen et al, 2017 ). Fermenters transform organic substrates, such as lactate, formate, and methanol, to acetate and hydrogen, which are used as a carbon source and electron donor, respectively, by dechlorinating microbes ( Wei and Finneran, 2013 ; Wen et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

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Effects of 1,1,1-Trichloroethane and Triclocarban on Reductive Dechlorination of Trichloroethene in a TCE-Reducing Culture

et al. 2017

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Chlorinated compounds were generally present in the environment due to widespread use in the industry. A short-term study was performed to evaluate the effects of 1,1,1- trichloroethane (TCA) and triclocarban (TCC) on trichloroethene (TCE) removal in a reactor fed with lactate as the sole electron donor. Both TCA and TCC inhibited TCE reduction, but the TCC had a more pronounced effect compared to TCA. The TCE-reducing culture, which had never been exposed to TCA before, reductively dechlorinated TCA to 1,1-dichloroethane (DCA). Below 15 μM, TCA had little effect on the transformation of TCE to cis-dichloroethene (DCE); however, the reduction of cis-DCE and vinyl chloride (VC) were more sensitive to TCA, and ethene production was completely inhibited when the concentration of TCA was above 15 μM. In cultures amended with TCC, the reduction of TCE was severely affected, even at concentrations as low as 0.3 μM; all the cultures stalled at VC, and no ethene was detected. The cultures that fully transformed TCE to ethene contained 5.2–8.1% Dehalococcoides. Geobacter and Desulfovibrio, the bacteria capable of partially reducing TCE to DCE, were detected in all cultures, but both represented a larger proportion of the community in TCC-amended cultures. All cultures were dominated by Clostridium_sensu_stricto_7, a genus that belongs to Firmicutes with proportions ranging from 40.9% (in a high TCC (15 μM) culture) to 88.2%. Methanobacteria was detected at levels of 1.1–12.7%, except in cultures added with 15 and 30 μM TCA, in which they only accounted for ∼0.4%. This study implies further environmental factors needed to be considered in the successful bioremediation of TCE in contaminated sites.

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“…The subsurface is a complex environment where OHRB can not only be challenged by cooccurring organohalogen mixtures, but other organic and inorganic co-contaminants such as heavy metals (e.g. Pb, Cd, Cr, Zn, Cu, Ni) (Bunge et al 2007, Costa and Jesus-Rydin 2001, Olaniran et al 2009, Subramanian et al 2015, (per)chlorate (Wen et al 2017), nitrous oxide (Yin et al 2019) and BTEX (benzene, toluene, ethylbenzene, xylenes) (Chen et al 2014b, Richmond et al 2001. Such co-contaminants normally not considered in studies focusing on dehalogenation may exert inhibitory/toxic effects on reductive dehalogenase (RDase)…”

Section: Future Perspectivesmentioning

confidence: 99%

Microbial transformation of organic and inorganic halogen compounds

Peng¹

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Haloalkanoates are environmental pollutants that can be degraded aerobically by microorganisms producing hydrolytic dehalogenases. However, there is lack of information about anaerobic degradation of haloalkanoates. Genome analysis of Pseudomonas chloritidismutans AW-1 T , a facultative anaerobic chlorate-reducing bacterium, showed presence of two putative haloacid dehalogenase genes, the L-DEX gene and dehI, encoding an L-2-haloacid dehalogenase (L-DEX) and a halocarboxylic acid dehydrogenase (DehI). Hence, we studied concurrent degradation of haloalkanoates and chlorate as a yet unexplored trait of strain AW-1 T. The deduced amino acid sequences of L-DEX and DehI revealed 33−37% and 26−86% similarities with biochemically/structurally characterized L-DEX and D-, DL-2haloacid dehalogenase enzymes, respectively. Physiological experiments confirmed that strain AW-1 T can grow on chloroacetate, bromoacetate and both Land D-α-halogenated propionates with chlorate as an electron acceptor. Interestingly, growth and haloalkanoates degradation were generally faster with chlorate as an electron acceptor than with oxygen. In line with this, analyses of L-DEX and DehI dehalogenase activities using cell free extract (CFE) of strain AW-1 T grown on DL-2-chloropropionate under chlorate-reducing condition showed up to 3.5-fold higher dehalogenase activity than the CFE obtained from cells grown on DL-2chloropropionate under aerobic condition. Reverse transcription quantitative PCR showed that the L-DEX gene was expressed constitutively independent of the electron donor (haloalkanoates or acetate) or acceptor (chlorate or oxygen), whereas expression of dehI was induced by haloalkanoates. Concurrent degradation of organic and inorganic halogenated compounds by strain AW-1 T represents a unique metabolic capacity in a single bacterium, providing a new piece in the puzzle of the microbial halogen cycle.

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The dechlorination of TCE by a perchlorate reducing consortium

Cited by 35 publications

References 55 publications

Preparation and characterization of site-specific dechlorinating microbial inocula capable of complete dechlorination enriched in anaerobic microcosms amended with clay mineral

Preparation and characterization of site-specific dechlorinating microbial inocula capable of complete dechlorination enriched in anaerobic microcosms amended with clay mineral

Effects of 1,1,1-Trichloroethane and Triclocarban on Reductive Dechlorination of Trichloroethene in a TCE-Reducing Culture

Microbial transformation of organic and inorganic halogen compounds

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