The Decline of Embryogenic Potential as Callus and Suspension Cultures of Carrot (Daucus carota L.) are Serially Subcultured

Smith, Susan; Street, H. E.

doi:10.1093/oxfordjournals.aob.a084807

Cited by 154 publications

(50 citation statements)

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“…It is possible that such cells arise from SM cells which become auxin-autotrophic and enlarge owing to the plasticizing effect of auxin on the cell wall (Wareing and Phillips, 1978). If this is indeed the case, then the commonly observed decline of EP of carrot cultures (Smith and Street, 1974) may be initiated by the appearance of auxin-autotrophic cells. These may be at a competitive advantage owing to the involvement of auxin in cell division (Bryant, 1976;p.…”

Section: Discussionmentioning

confidence: 99%

“…The appearance of embryo-like structures in newly initiated, plus-auxin cultures is commonly observed and it appears that the freshly isolated cells are less sensitive to inhibition of embryogenesis by auxin (Smith and Street, 1974). As the number of such structures is low, their enlarged nature could also result from a slow rate of equilibration of some factor with the medium.…”

Section: Discussionmentioning

confidence: 99%

“…Embryogenic potential (EP) is used in the sense defined by Smith and Street (1974). Cultures of 'high EP' showed normal embryo formation from 60 to 80% of the SM cell clusters present.…”

Section: Methodsmentioning

confidence: 99%

“…Warren and Fowler (1978) observed two distinct but parallel pathways of development in cultures of Daucus carota induced to form embryos. One results in the formation of 'normal' viable embryos (Smith and Street, 1974;Warren and Fowler, 1977), and the other in large globular embryo-like but abnormal structures. Under other conditions, embryo death after the heart stage occurs.…”

Section: Introductionmentioning

confidence: 99%

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PHYSIOLOGICAL INTERACTIONS DURING THE INITIAL STAGES OF EMBRYOGENESIS IN CULTURES OF DAUCUS CAROTA L.

Warren¹,

Fowler²

1981

New Phytologist

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SUMMARY Somatic globular embryos of carrot develop abnormally when transferred at low density to fresh, minus‐auxin medium, whereas normal development ensues after transfer to conditioned medium, or to fresh medium at high densities. It is suggested that a critical level of an ‘embryo factor’ is required to co‐ordinate cell division and morphogenesis. Embryo death occurs after the heart stage in minus‐auxin cultures of low embryogenic potential. This behaviour may result from the production of auxin as these cultures show some plus‐auxin characteristics. The decline in the capacity for normal embryogenesis which often accompanies repeated subculture is suggested to result, in part, from a combination of these factors.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Embryogenic potential (EP) is used in the sense defined by Smith and Street (1974). Cultures of 'high EP' showed normal embryo formation from 60 to 80% of the SM cell clusters present.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

PHYSIOLOGICAL INTERACTIONS DURING THE INITIAL STAGES OF EMBRYOGENESIS IN CULTURES OF DAUCUS CAROTA L.

Warren¹,

Fowler²

1981

New Phytologist

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“…Il est également possible que l'on ait progressivement sélectionné des cellules aneuploïdes et polyploïdes qui se trouvent souvent avantagées en culture mixte (Smith et Street, 1974 ;Singh et Coll., 1975), soit qu'elles utilisent mieux les glucides du milieu, soit qu'elles présentent une moins grande sensibilité à un facteur limitant comme c'est le cas dans certaines cultures de Carotte (Rayliss, 1977). Un changement du taux de ploïdic expliquerait également que la lignée B-22 ne produise plus de racines,ni de bourgeons.…”

Section: Pourcentage De Cals Lignees Milieux Organogénesunclassified