The decreased membrane fluidity of in vivo aged, human erythrocytes. A spin label study

Shiga, Tohru; Maeda, Nobuji; Suda, Takeo; Kon, Kazunori; Sekiya, Misuzu

doi:10.1016/0005-2736(79)90032-4

Cited by 93 publications

(18 citation statements)

References 40 publications

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“…These results, ex pressed per milligram of erythrocyte mem brane protein, indicate a significant decrease of the membrane lipid to protein ratio which would be consistent with the hypothesized release of protein-poor vesicles from the cells. The data shown, as well as other results [32, 45,46], point to a lack of significant changes in the cholesterokphospholipid ratio although some authors reported an increase on this ratio in oldest mammalian red cells [47,48], Marin et al [48] recently presented data indicating a significant increase in the cholesterokphospholipid ratio in the densest fractions of ovine erythrocytes. They found the cholesterokphospholipid molar ratio to be 1.01 ± 0.04 in cells of density <1.100 g/ cm3, not to change significantly till the den sity of 1.115 g/cm3 but to increase up to 1.40 ± 0.49 in cells of density between 1.115 and I .…”

Section: Lipid Compositionsupporting

confidence: 51%

“…4) and the occurrence of changes (especially of an increase in the phosphatidylserine/sphingomyelin ratio) was recently communicated for ovine (Ovis aries L.) cells [48], No gross changes in the composition of fatty acid residues were noted to accompany cell aging except for a small increase in the content of linoleic acid residues and a slight decrease in the content of arachidonic acid residues [42,45,50,51]. According to van Gastel et ah [42], the con tent of linoleic acid residues ( C^) increases from 15.2% for 14% lightest cells to 17.9% for 14% densest cells which is accompanied by a decrease in the content of arachidonic acid residues (C20:4) from 19.2 to 16.3%.…”

Section: Lipid Compositionmentioning

confidence: 99%

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Erythrocyte Aging: Physical and Chemical Membrane Changes

Bartosz¹

1991

Gerontology

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The mammalian erythrocyte is an interesting model for studies of membrane aging. Experimental approaches to this problem involve, first of all, comparison of properties of erythrocytes separated by density because red blood cell age correlates with density in principle. Other approaches to study red cell membrane aging, such as hypertransfusion, are also discussed. A number of physical and chemical changes occur in erythrocytes with aging. Crucial to the elucidation of aging mechanisms is to determine which are primary and which are secondary. Immunoglobulin G binding triggers cellular removal and seems necessary for the recognition of senescent erythrocytes. Cellular deformability decreases while fragility to hemolytic factors generally increases and surface charge density does not alter. Perhaps more important are reactions of reactive oxygen species with membrane constituents and subsequent proteolysis. These are implicated as causative factors in red cell aging, immunoglobulin binding and recognition of senescent erythrocytes.

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Section: Lipid Compositionsupporting

confidence: 51%

Section: Lipid Compositionmentioning

confidence: 99%

Erythrocyte Aging: Physical and Chemical Membrane Changes

Bartosz¹

1991

Gerontology

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“…Measurements of enzyme activities generally considered as markers of the mitochondrial, plasma-membrane, and ER membranes (14,18) (20). Reduced fluidity of plasma membranes with age appears to be a general phenomenon and has also been demonstrated for both bacterial (1) and animal cell membranes (23).…”

Section: Resultsmentioning

confidence: 99%

Senescence and the Fluidity of Rose Petal Membranes

Borochov¹,

Halevy²,

Shinitzky³

1982

Plant Physiol.

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In previous work, senescence of rose petal cells has been shown to be accompanied by a gradual decrease of membrane fluidity, as measured by a fluorescence polarization technique. Concomitantly, an increase in the free sterol-to-phospholipid ratio was found. Both observations were verified in this study. Further, experiments carried out on whole tissue and isolated protoplasts during senescence revealed that there was no quantitative change in the level of free sterols. The content of phospholipids decreased without any significant change in their composition. Results from experiments measuring the incorporation of [(32)P]orthophosphate indicated a reduced capacity for phospholipid synthesis in senescent cells. Both young and old tissue showed phospholipase A and D activity, the former increasing with age.It was concluded that the fluidity of rose petal membranes decreases with age as a result of a decrease in phospholipid content, brought about by both reduced synthesis and enhanced degradation. Evidence supporting the view that the phenomena observed are related specifically to changes in the plasmalemma is discussed.

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“…Several parame ters change during the erythrocyte life [8,22], and the erythrocyte enzymes also vary in their activity with the age of the whole organ ism [13]. A study on the ATP metabolism is presented in this paper, since an intact ATP metabolism holds an existential importance for the red blood cell.…”

mentioning

confidence: 99%