The deduced sequence of the transcription factor TFIIIA from Saccharomyces cerevisiae reveals extensive divergence from Xenopus TFIIIA.

Archambault, Jacques; Milne, Cheryl; Schappert, Keith; Baum, Bobby; Friesen, James D.; Segall, J

doi:10.1016/s0021-9258(19)50728-1

Cited by 63 publications

(6 citation statements)

References 61 publications

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“…Yeast genetic techniques and media were as described by Ausubel et al (1987). The wild-type strain YRW1 was MATa can1-100 his3-11 leu2-3,112 trp1-1 ura3-1 ade2-1 tfc2::LEU2 pJA230 ( URA3 , TFC2 , and CEN3 ) (Archambault et al, 1992). The YSC14 strain was derived from YRW1; it survived without the TFIIIA factor because of the presence of a multicopy plasmid (pRSC3) containing a 5S RNA gene under the control of the RNA polymerase III RPR1 promoter as described in Camier et al (1995).…”

Section: Strains Plasmids and Mediamentioning

confidence: 99%

Assembly of 5S Ribosomal RNA Is Required at a Specific Step of the Pre-rRNA Processing Pathway

Dechampesme

Koroleva

Léger-Silvestre

et al. 1999

The Journal of Cell Biology

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A collection of yeast strains surviving with mutant 5S RNA has been constructed. The mutant strains presented alterations of the nucleolar structure, with less granular component, and a delocalization of the 25S rRNA throughout the nucleoplasm. The 5S RNA mutations affected helix I and resulted in decreased amounts of stable 5S RNA and of the ribosomal 60S subunits. The shortage of 60S subunits was due to a specific defect in the processing of the 27SB precursor RNA that gives rise to the mature 25S and 5.8S rRNA. The processing rate of the 27SB pre-rRNA was specifically delayed, whereas the 27SA and 20S pre-rRNA were processed at a normal rate. The defect was partially corrected by increasing the amount of mutant 5S RNA. We propose that the 5S RNA is recruited by the pre-60S particle and that its recruitment is necessary for the efficient processing of the 27SB RNA precursor. Such a mechanism could ensure that all newly formed mature 60S subunits contain stoichiometric amounts of the three rRNA components.

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Section: Strains Plasmids and Mediamentioning

confidence: 99%

Assembly of 5S Ribosomal RNA Is Required at a Specific Step of the Pre-rRNA Processing Pathway

Dechampesme

Koroleva

Léger-Silvestre

et al. 1999

The Journal of Cell Biology

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“…The combination in mZl 3 and cZl 3 of a 12 zinc-finger 'hand' and an additional isolated finger is unusual. A similar unconventional arrangement in the Saccharomyces cerevisiae TFIIIA [31] and murine (DAP3 [32] proteins has been previously described. However, unlike Z13, the yeast TFIIIA spacer region is not a conserved feature of TFIIIA genes and therefore is not likely to exhibit functional significance.…”

Section: Discussionmentioning

confidence: 59%

An unusual arrangement of 13 zinc fingers in the vertebrate gene Z13

Schulz

Hopwood

Rathjen

et al. 1995

Biochemical Journal

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The zinc finger is a protein domain that imparts specific nucleic acid-binding activity on a wide range of functionally important proteins. In this paper we report the molecular cloning and characterization of a novel murine zinc-finger gene, mZ13. Analysis of mZ13 cDNAs revealed that the gene expresses a 794-amino-acid protein encoded by a 2.7 kb transcript. The protein has an unusual arrangement of 13 zinc fingers into a 'hand' of 12 tandem fingers and a single isolated finger near the C-terminus. This structural organization is conserved with the probable chicken homologue, cZ13. mZ13 also contained an additional domain at the N-terminus which has previously been implicated in the regulation of zinc-finger transcription factor DNA-binding, via protein-protein interactions. mZ13 expression was detected in a wide range of murine embryonic and adult tissues. The structural organization of mZ13 and its expression profile suggest that it may function as a housekeeping DNA-binding protein that regulates the expression of specific genes.

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“…In contrast, human cell extracts are less selective in terms of species specificity and can accurately transcribe and process yeast tRNA genes (14,45). Intriguingly, the sequences of yeast and vertebrate TFIIIA have also been shown to be poorly conserved (1). Although they both contain nine zinc fingers, S. cerevisiae and X. laevis TFIIIA are only -20% identical; if the seven consensus amino acids of the zinc finger motif are excluded, the identity decreases to -8%.…”

Section: Discussionmentioning

confidence: 99%

Cloning and Characterization of an Evolutionarily Divergent DNA-Binding Subunit of Mammalian TFIIIC

Lagna

Kovelman

Sukegawa

et al. 1994

Molecular and Cellular Biology

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Transcription factor IIIC (TFIIIC) is required for the assembly of a preinitiation complex on 5S RNA, tRNA, and adenovirus VA RNA genes and contains two separable components, TFIIIC1 and TFIIIC2. TFIIIC2 binds to the 3' end of the internal control region of the VAI RNA gene and contains five polypeptides ranging in size from 63 to 220 kDa; the largest of these directly contacts DNA. Here we describe the cloning of cDNAs encoding all (rat) or part (human) of the 220-kDa subunit (TFIIIC alpha). Surprisingly, TFIIIC alpha has no homology to any of the yeast TFIIIC subunits already cloned, suggesting a significant degree of evolutionary divergence for RNA polymerase III factors. Antibodies raised against the N terminus of recombinant human TFIIIC alpha specifically inhibit binding of natural TFIIIC to DNA. Furthermore, immunodepletion assays indicate that TFIIIC alpha is absolutely required for RNA polymerase III transcription of 5S RNA, tRNA, and VAI RNA genes but not for the 7SK RNA and U6 small nuclear RNA genes. Transcription from the tRNA and VAI RNA genes in TFIIIC-depleted nuclear extracts can be restored by addition of purified TFIIIC. In contrast, restoration of 5S RNA gene transcription requires readdition of both TFIIIC and TFIIIA, indicating a promoter-independent interaction between these factors. Immunoprecipitation experiments demonstrate a tight association of all five polypeptides previously identified in the TFIIIC2 fraction, confirming the multisubunit structure of the human factor.

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The deduced sequence of the transcription factor TFIIIA from Saccharomyces cerevisiae reveals extensive divergence from Xenopus TFIIIA.

Cited by 63 publications

References 61 publications

Assembly of 5S Ribosomal RNA Is Required at a Specific Step of the Pre-rRNA Processing Pathway

Assembly of 5S Ribosomal RNA Is Required at a Specific Step of the Pre-rRNA Processing Pathway

An unusual arrangement of 13 zinc fingers in the vertebrate gene Z13

Cloning and Characterization of an Evolutionarily Divergent DNA-Binding Subunit of Mammalian TFIIIC

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