The degeneration of biological cardiovascular prostheses under pro-calcific metabolic conditions in a small animal model

Assmann, Alexander; Zwirnmann, Kai; Heidelberg, Friederike; Schiffer, Franziska; Horstkötter, Kim; Munakata, Hiroshi; Gremse, Felix; Barth, Mareike; Lichtenberg, Artur; Akhyari, Payam

doi:10.1016/j.biomaterials.2014.05.034

Cited by 36 publications

(41 citation statements)

References 45 publications

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“…Histology and immunohistology were conducted on paraformaldehyde-fixed 4-6 μm paraffin and cryo-sections of the explants as previously reported [42]. Hematoxylin/eosin staining was performed to obtain microscopic overview.…”

Section: Methodsmentioning

confidence: 99%

A highly adhesive and naturally derived sealant

et al. 2017

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Conventional surgical techniques to seal and repair defects in highly stressed elastic tissues are insufficient. Therefore, this study aimed to engineer an inexpensive, highly adhesive, biocompatible, and biodegradable sealant based on a modified and naturally derived biopolymer, gelatin methacryloyl (GelMA). We tuned the degree of gelatin modification, prepolymer concentration, photoinitiator concentration, and crosslinking conditions to optimize the physical properties and adhesion of the photocrosslinked GelMA hydrogels. Following ASTM standard tests that target wound closure strength, shear resistance, and burst pressure, GelMA sealant was shown to exhibit adhesive properties that were superior to clinically used fibrin- and poly(ethylene glycol)-based glues. Chronic in vivo experiments in small as well as translational large animal models proved GelMA to effectively seal large lung leakages without the need for sutures or staples, presenting improved performance as compared to fibrin glue, poly(ethylene glycol) glue and sutures only. Furthermore, high biocompatibility of GelMA sealant was observed, as evidenced by a low inflammatory host response and fast in vivo degradation while allowing for adequate wound healing at the same time. Combining these results with the low costs, ease of synthesis and application of the material, GelMA sealant is envisioned to be commercialized not only as a sealant to stop air leakages, but also as a biocompatible and biodegradable hydrogel to support lung tissue regeneration.

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Section: Methodsmentioning

confidence: 99%

A highly adhesive and naturally derived sealant

et al. 2017

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“…Histology and Immunohistology : Histological analyses were performed on 6 μm cryo‐sections of the explanted ELP samples. After fixation with paraformaldehyde, hematoxylin, and eosin staining was conducted as previously reported . Immunohistological staining was performed as previously reported .…”

Section: Methodsmentioning

confidence: 99%

A Highly Elastic and Rapidly Crosslinkable Elastin‐Like Polypeptide‐Based Hydrogel for Biomedical Applications

Zhang

Avery

Vallmajó-Martín

et al. 2015

Adv Funct Materials

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Elastin-like polypeptides (ELPs) are promising for biomedical applications due to their unique thermoresponsive and elastic properties. ELP-based hydrogels have been produced through chemical and enzymatic crosslinking or photocrosslinking of modified ELPs. Herein, a photocrosslinked ELP gel using only canonical amino acids is presented. The inclusion of thiols from a pair of cysteine residues in the ELP sequence allows disulfide bond formation upon exposure to UV light, leading to the formation of a highly elastic hydrogel. The physical properties of the resulting hydrogel such as mechanical properties and swelling behavior can be easily tuned by controlling ELP concentrations. The biocompatibility of the engineered ELP hydrogels is shown in vitro as well as corroborated in vivo with subcutaneous implantation of hydrogels in rats. ELP constructs demonstrate long-term structural stability in vivo, and early and progressive host integration with no immune response, suggesting their potential for supporting wound repair. Ultimately, functionalized ELPs demonstrate the ability to function as an in vivo hemostatic material over bleeding wounds.

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“…Hematoxylin/eosin (H&E) staining and immunohistology were performed on 6 μm cryosections of the frozen tissues according to standard protocols. 28 For immunohistology, cryosections were first fixed in formalin. This was followed by permeabilization with 0.25% Triton-X, blocking and treatment with primary antibodies: mouse monoclonal anti-rat CD 3 (1:100) and rabbit monoclonal anti-rat CD 68 (1:100).…”

Section: Methodsmentioning

confidence: 99%

Nanoengineered biomimetic hydrogels for guiding human stem cell osteogenesis in three dimensional microenvironments

et al. 2016

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The ability to modulate stem cell differentiation in a three dimensional (3D) microenvironment for bone tissue engineering in absence of exogenous pharmaceutical agents such as bone morphogenic protein (BMP-2) remains a challenge. In this study, we introduce extracellular matrix (ECM)-mimicking nanocomposite hydrogels to induce osteogenic differentiation of human mesenchymal stem cells (hMSCs) for bone regeneration in absence of any osteoinducting factors. In particular, we have reinforced photocrosslinkable collagen-based matrix (gelatin methacryloyl, GelMA) used disk-shaped nanosilicates (nSi), a new class of two-dimensional (2D) nanomaterials. We show that nanoengineered hydrogels supported migration and proliferation of encapsulated hMSCs, with no signs of cell apoptosis or inflammatory cytokine responses. The addition of nSi significantly enhances osteogenic differentiation of encapsulated hMSCs as evident by the increase in alkaline phosphates (ALP) activity and deposition of biomineralized matrix compared to GelMA without nSi. We also show that microfabricated nanoengineered microgels can be used to pattern and control cellular behaviour. Furthermore, we also show that nanoengineered hydrogel have high biocompatibility as determined by in vivo experiments using immunocompetent rat model. Specifically, the hydrogels showed minimum localized immune responses, indicating it ability for tissue engineering applications. Overall, we showed the ability of nanoengineered hydrogels loaded with 2D nanosilicates for osteogenic differentiation of stem cells in vitro, in absence of any growth factors such as BMP-2. Our in vivo studies show high biocompatibility of nanocomposites and show the potential for growth factor free bone regeneration.

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The degeneration of biological cardiovascular prostheses under pro-calcific metabolic conditions in a small animal model

Cited by 36 publications

References 45 publications

A highly adhesive and naturally derived sealant

A highly adhesive and naturally derived sealant

A Highly Elastic and Rapidly Crosslinkable Elastin‐Like Polypeptide‐Based Hydrogel for Biomedical Applications

Nanoengineered biomimetic hydrogels for guiding human stem cell osteogenesis in three dimensional microenvironments

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