The degradation fragments of gamma-glutamyl transpeptidase from Bacillus subtilis BU108 have antimicrobial activity against Streptomyces scabiei

Liu, Quan; Shen, Yongrui; Yin, Kuide

doi:10.1007/s41348-020-00371-5

Cited by 3 publications

(5 citation statements)

References 28 publications

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“…In a recent report, degradation fragments of GGT from B. subtilis BU108 have been demonstrated to have antimicrobial activity against Streptomyces scabiei (Liu et al, 2020). Two antimicrobial peptides (AMPs), viz KT20 (KKGGNAIDAAVAIQFALNVT) and IF20 (IQKDLAKTFKLIRSNGTDAF), have been identified, which are the degradation fragments of GGT enzymes.…”

Section: Allied Applications Of Microbial Ggtsmentioning

confidence: 99%

Bacterial Gamma-Glutamyl Transpeptidase, an Emerging Biocatalyst: Insights Into Structure–Function Relationship and Its Biotechnological Applications

et al. 2021

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Gamma-glutamyl transpeptidase (GGT) enzyme is ubiquitously present in all life forms and plays a variety of roles in diverse organisms. Higher eukaryotes mainly utilize GGT for glutathione degradation, and mammalian GGTs have implications in many physiological disorders also. GGTs from unicellular prokaryotes serve different physiological functions in Gram-positive and Gram-negative bacteria. In the present review, the physiological significance of bacterial GGTs has been discussed categorizing GGTs from Gram-negative bacteria like Escherichia coli as glutathione degraders and from pathogenic species like Helicobacter pylori as virulence factors. Gram-positive bacilli, however, are considered separately as poly-γ-glutamic acid (PGA) degraders. The structure–function relationship of the GGT is also discussed mainly focusing on the crystallization of bacterial GGTs along with functional characterization of conserved regions by site-directed mutagenesis that unravels molecular aspects of autoprocessing and catalysis. Only a few crystal structures have been deciphered so far. Further, different reports on heterologous expression of bacterial GGTs in E. coli and Bacillus subtilis as hosts have been presented in a table pointing toward the lack of fermentation studies for large-scale production. Physicochemical properties of bacterial GGTs have also been described, followed by a detailed discussion on various applications of bacterial GGTs in different biotechnological sectors. This review emphasizes the potential of bacterial GGTs as an industrial biocatalyst relevant to the current switch toward green chemistry.

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Section: Allied Applications Of Microbial Ggtsmentioning

confidence: 99%

Bacterial Gamma-Glutamyl Transpeptidase, an Emerging Biocatalyst: Insights Into Structure–Function Relationship and Its Biotechnological Applications

et al. 2021

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“…Bacillus velezensis BAC03 effectively controls common scab of potato due to the production of LCI polypeptide [ 8 , 67 ] and endophytic bacteria Bacillus velezensis strain 8–4 has a strong inhibitory effect on various pathogens, such as Streptomyces galilaeus , Phoma foveat, Rhizoctonia solani, Fusarium avenaceum and Colletotrichum coccodes [ 118 ]. Bacillus subtilis suppresses Streptomyces scabies by producing AMEP412, a protein elicitor with antimicrobial activity [ 119 ]. The degradation fragments of gamma–glutamyl transpeptidase from Bacillus subtilis BU108 have antimicrobial activity against Streptomyces scabies [ 119 ], and secondary metabolites such as surfactin, iturin A, and fengycin are commonly reported as antimicrobial substances [ 120 ].…”

Section: Soil Amendment For Disease Management—from Practice To Promisementioning

confidence: 99%

“…Bacillus subtilis suppresses Streptomyces scabies by producing AMEP412, a protein elicitor with antimicrobial activity [ 119 ]. The degradation fragments of gamma–glutamyl transpeptidase from Bacillus subtilis BU108 have antimicrobial activity against Streptomyces scabies [ 119 ], and secondary metabolites such as surfactin, iturin A, and fengycin are commonly reported as antimicrobial substances [ 120 ]. Pseudomonas synxantha LBUM223 applied in the field suppresses common scab of potato [ 68 ].…”

Section: Soil Amendment For Disease Management—from Practice To Promisementioning

confidence: 99%

Irreplaceable Role of Amendment-Based Strategies to Enhance Soil Health and Disease Suppression in Potato Production

Hao

Ashley

2021

Microorganisms

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Soilborne diseases are a major constraining factor to soil health and plant health in potato production. In the toolbox of crop management, soil amendments have shown benefits to control these diseases and improve soil quality. Most amendments provide nutrients to plants and suppress multiple soilborne pathogens. Soil amendments are naturally derived materials and products and can be classified into fresh or living plants, organic or inorganic matters, and microbial supplements. Fresh plants have unique functions and continuously exude chemicals to interact with soil microbes. Organic and inorganic matter contain high levels of nutrients, including nitrogen and carbon that plants and soil microorganisms need. Soil microorganisms, whether being artificially added or indigenously existing, are a key factor in plant health. Microbial communities can be considered as a biological reactor in an ecosystem, which suppress soilborne pathogens in various mechanisms and turn soil organic matter into absorbable forms for plants, regardless of amendment types. Therefore, soil amendments serve as an energy input, nutrient source, and a driving force of microbial activities. Advanced technologies, such as microbiome analyses, make it possible to analyze soil microbial communities and soil health. As research advances on mechanisms and functions, amendment-based strategies will play an important role in enhancing soil health and disease suppression for better potato production.

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“…Phenazine-1-carboxylic acid production => 12%-14% of all S. scabiei genes were differentially expressed, including key genes involved in pathogenicity/virulence, mycelium differentiation and increased oxidative stress [164] Fragments of γ-glutamyl transpeptidase from Bacillus subtilis BU108 In vitro growth inhibition of S. scabiei [165] S. melanosporofaciens EF-76 Geldanamycin production => disease index was reduced from 6.30 to 4.81 and from 2.83 to 2.49 in growth chamber and field experiments, respectively [166] Streptomyces A1RT Production of isatropolone C and indole-3-acetic acid => reduction in average disease severity index by 82.4-95.7% [4] Streptomyces isolates (strains 93 and 63) and their spontaneous mutants…”

Section: Pseudomonas Fluorescens Lbum223mentioning

confidence: 99%

“…No disease symptoms in planta, altered morphological differentiation-quorum quenching paralyzing γ-butyrolactone signaling pathway [161] The variation in the microbial community response might be connected to the mechanisms of suppression provided by the inoculated strains. Mostly, production of antibiotics against the pathogens is involved, while the identified compounds include geldanamycin [166], phenazine-1-carboxylic acid [163,164], isatropolone C [4], azalomycin [145] or antimicrobial peptides [154,165]. However, the strains also produce compounds such as indole-3-acetic acid or siderophores and enable nitrogen fixation and phosphate solubilization [145] or remove signaling through γ-butyrolactone pathway [161].…”

Section: Pseudomonas Fluorescens Lbum223mentioning

confidence: 99%

Micronutrients and Soil Microorganisms in the Suppression of Potato Common Scab

et al. 2021

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Nature-friendly approaches for crop protection are sought after in the effort to reduce the use of agrochemicals. However, the transfer of scientific findings to agriculture practice is relatively slow because research results are sometimes contradictory or do not clearly lead to applicable approaches. Common scab of potatoes is a disease affecting potatoes worldwide, for which no definite treatment is available. That is due to many complex interactions affecting its incidence and severity. The review aims to determine options for the control of the disease using additions of micronutrients and modification of microbial communities. We propose three approaches for the improvement by (1) supplying soils with limiting nutrients, (2) supporting microbial communities with high mineral solubilization capabilities or (3) applying communities antagonistic to the pathogen. The procedures for the disease control may include fertilization with micronutrients and appropriate organic matter or inoculation with beneficial strains selected according to local environmental conditions. Further research is proposed to use metagenomics/metabolomics to identify key soil–plant–microbe interactions in comparisons of disease-suppressive and -conducive soils.

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The degradation fragments of gamma-glutamyl transpeptidase from Bacillus subtilis BU108 have antimicrobial activity against Streptomyces scabiei

Cited by 3 publications

References 28 publications

Bacterial Gamma-Glutamyl Transpeptidase, an Emerging Biocatalyst: Insights Into Structure–Function Relationship and Its Biotechnological Applications

Bacterial Gamma-Glutamyl Transpeptidase, an Emerging Biocatalyst: Insights Into Structure–Function Relationship and Its Biotechnological Applications

Irreplaceable Role of Amendment-Based Strategies to Enhance Soil Health and Disease Suppression in Potato Production

Micronutrients and Soil Microorganisms in the Suppression of Potato Common Scab

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