The degradation of n-hexadecane in soil by thermophilic geobacilli

Marchant, Roger; Sharkey, Freddie H.; Banat, Ibrahim M.; Rahman, Thahira; Perfumo, Amedea

doi:10.1111/j.1574-6941.2006.00061.x

Cited by 65 publications

(47 citation statements)

References 27 publications

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“…More recently, Bacillus, Geobacillus (phylum Firmicutes) and Thermus (phylum Deinococcus-Thermus) isolates were found to degrade alkanes (Marchant et al, 2006;Meintanis et al, 2006). The best diesel degrading Gram-positive microorganisms (D2, D9, D10) isolated in this study were identified as Paenibacillus sp.…”

Section: Discussionmentioning

confidence: 90%

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Diesel degradation and biosurfactant production by Gram-positive isolates

Ganesh¹,

Lin²

2009

Afr. J. Biotechnol.

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Accidental leakages during hydrocarbon fuels transportation and other activities are inevitable, making these hydrocarbons the most common global environmental pollutants. Current understanding in diesel degradation involves the study of Gram-negative microorganisms. Seven Gram-positive and one Gram-negative diesel degrading bacteria isolated from contaminated soil were used in this study. The overnight bacterial cultures were standardized and transferred into Bushnell-Haas medium supplemented with glucose and incubated at 30°C at 160 rpm for 48 h. The level of diesel degradation was determined using gravimetric analysis. Cell numbers were calculated using total heterotrophic plate count. All isolates were capable of degrading 70 -80% of n-paraffin whilst isolates D2, D9, D10 and DJLB possessed better abilities of diesel degradation at 65.4 -83.12% under the standard conditions. Diesel degradation rates and microbial cell number, increased with an increase in glucose composition. The addition of glucose to the liquid medium had a positive effect, with an increase in growth of the isolates thus leading to significantly (p < 0.05) higher percentages of diesel degradation and greater emulsification activity. The ability of Gram-positive bacteria to degrade diesel increased in a comparable trend as its biosurfactant production increased. The E 24 index was highest at 87.6% for isolate D9. Isolates D2, D9 and D10, were identified as Paenibacillus sp. whilst isolate DJLB was found to belong to Stenotrophomonas sp. This study clearly demonstrates that Gram-positive biosurfactant producing bacteria are effective in diesel degradation.

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Section: Discussionmentioning

confidence: 90%

“…Microorganisms capable of surviving on these highly reduced organic compounds have been identified (Marchant et al, 2006). More recently, Bacillus, Geobacillus (phylum Firmicutes) and Thermus (phylum Deinococcus-Thermus) isolates were found to degrade alkanes (Marchant et al, 2006;Meintanis et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Diesel degradation and biosurfactant production by Gram-positive isolates

Ganesh¹,

Lin²

2009

Afr. J. Biotechnol.

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“…pGAM47-bgaB pUC19 derivative; pUC replicon, oriT, bla, pyrF, P sigA -bgaB lanked by gk0707 upstream and downstream regions [19] pGAM48-bgaB pUC19 derivative; pUC replicon, oriT, bla, pyrF, P gk704 -bgaB lanked by gk0707 upstream and downstream regions [21] pGKE25 pUC19 derivative; pUC replicon, oriT, bla, pyrF [20] pGKE25-bgaB pGKE25 derivative; pUC replicon, oriT, bla, pyrF, P sigA -bgaB lanked by gkp09 upstream and downstream regions This study pUCG18T pUC18 derivative; pUC and pBST1 replicons, oriT, bla, TK101…”

Section: Relevant Description Referencementioning

confidence: 99%

“…The whole genome sequence has been determined [17], showing that G. kaustophilus HTA426 harbors the circular plasmid pHTA426 (Figure 1). Because the members of the genus Geobacillus include strains that are useful for high-temperature processes, as demonstrated by strains that are capable of degrading hydrocarbons [18], long-chain alkanes [19][20][21], biphenyls [22], parain-wax [23], or nylons [24], we have studied biotechnological applications of the genus using G. kaustophilus HTA426 as a model and pilot strain.…”

Section: Archaea -New Biocatalysts Novel Pharmaceuticals and Variousmentioning

confidence: 99%

Plasmid Curing is a Promising Approach to Improve Thermophiles for Biotechnological Applications: Perspectives in Archaea

Mizuno¹,

Ohshiro²,

Suzuki³

2017

Archaea - New Biocatalysts, Novel Pharmaceuticals and Various Biotechnological Applications

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Thermophiles are atractive as host cells for microbial processes to produce or degrade various compounds. In these applications, it is often desirable to improve the properties of thermophiles, such as their growth rate, cell density, and protein productivity, although this is rarely achieved because of the lack of general approaches. In this chapter, we describe the elimination of the pHTA426 plasmid from a moderate thermophile, Geobacillus kaustophilus HTA426, and its efects on the microbial properties. This process, called plasmid curing, was simply achieved using a DNA intercalator and conirmed by phenotypic and genotypic analyses. Of note, pHTA426 curing had beneicial efects on diverse properties, probably because of the reduced energy burden in terms of plasmid replication at high temperatures. The result suggests that plasmid curing is a simple and versatile approach for improving thermophiles. In particular, this approach may be efective for archaeal thermophiles because they grow at much higher temperatures and could have the greater energy burden on plasmid replication. Data mining has also shown that plasmids are distributed in archaeal thermophiles. This chapter provides a new tip for improving archaeal thermophiles, thereby increasing the opportunities for their use in various biotechnological applications.

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“…We will also investigate the aspect of production of biosurfactants as an additional property of some thermophilic degrader microorganisms for further enhancing hydrocarbon removal. Some of these aspects have been introduced in recent publications [17][18][19]26]. …”

Section: Introductionmentioning

confidence: 99%

The use of thermophilic bacteria in accelerated hydrocarbon bioremediation

Perfumo¹,

Banat²,

Marchant³

2006

WIT Transactions on Ecology and the Environment, Vol 88

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Successful bioremediation of hydrocarbon contamination in terrestrial as well as aquatic ecosystems is generally limited by the low bioavailability of hydrophobic pollutants. Considerably higher mass transfer rates and hydrocarbon solubilities can be obtained at higher temperatures, but so far approaches to bioremediation at increased temperature have hardly been investigated. The biotechnological significance and the benefits of the use of thermophilic bacteria in enhancing hydrocarbon removal under different environmental conditions were investigated. Expression of the alkane mono-oxygenase alkB gene responsible for the initial step in the degradation of alkanes in soil geobacilli was found to be induced by the presence of such a substrate at high temperature, and this explains the increased degradation rates achieved in hexadecane-contaminated microcosms incubated at 60°C compared to mesophilic conditions. Additional benefits of increasing hydrocarbon degradation also occur when thermophilic bacteria capable of producing biosurfactants which act as solubilising agents in such systems are introduced. We conclude that thermally accelerated bioremediation may be an effective technology for hydrocarbon contaminated soil bioremediation.

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The degradation of n-hexadecane in soil by thermophilic geobacilli

Cited by 65 publications

References 27 publications

Diesel degradation and biosurfactant production by Gram-positive isolates

Diesel degradation and biosurfactant production by Gram-positive isolates

Plasmid Curing is a Promising Approach to Improve Thermophiles for Biotechnological Applications: Perspectives in Archaea

The use of thermophilic bacteria in accelerated hydrocarbon bioremediation

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