The Degree of Breath Methane Production in IBS Correlates With the Severity of Constipation

Chatterjee, Soumya; Park, Sandy; Low, Kimberly; Kong, Yuthana; Pimentel, Mark

doi:10.1111/j.1572-0241.2007.01072.x

Cited by 189 publications

(164 citation statements)

References 22 publications

Supporting

Mentioning

144

Contrasting

Unclassified

Order By: Relevance

“…Elevated levels of fermentation intermediates in IBS stools might point to a disbalance of the gut environment and intestinal microbiota in this disease, leading to reduced metabolite cross-feeding among microbes and a resulting incomplete fermentation process. Because formate is utilized in the gut by methanogenic microbes, increased fecal formate is consistent with a lower methanogenic activity in the IBS-D lumen (Chatterjee et al, 2007). And as intestinal methane adversely affects gut transit time Fecal microbe-metabolite associations in health and IBS V Shankar et al (Ghoshal et al, 2011), lower methane production in IBS-D would facilitate faster gut transit resulting in a more frequent bowel movement.…”

Section: Discussionmentioning

confidence: 97%

The networks of human gut microbe–metabolite associations are different between health and irritable bowel syndrome

et al. 2015

View full text Add to dashboard Cite

The goal of this study was to determine if fecal metabolite and microbiota profiles can serve as biomarkers of human intestinal diseases, and to uncover possible gut microbe-metabolite associations. We employed proton nuclear magnetic resonance to measure fecal metabolites of healthy children and those diagnosed with diarrhea-predominant irritable bowel syndrome (IBS-D). Metabolite levels were associated with fecal microbial abundances. Using several ordination techniques, healthy and irritable bowel syndrome (IBS) samples could be distinguished based on the metabolite profiles of fecal samples, and such partitioning was congruent with the microbiota-based sample separation. Measurements of individual metabolites indicated that the intestinal environment in IBS-D was characterized by increased proteolysis, incomplete anaerobic fermentation and possible change in methane production. By correlating metabolite levels with abundances of microbial genera, a number of statistically significant metabolite-genus associations were detected in stools of healthy children. No such associations were evident for IBS children. This finding complemented the previously observed reduction in the number of microbe-microbe associations in the distal gut of the same cohort of IBS-D children.

show abstract

Section: Discussionmentioning

confidence: 97%

The networks of human gut microbe–metabolite associations are different between health and irritable bowel syndrome

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Repeat breath samples were then obtained 15 minutes after lactulose ingestion, and levels of methane and hydrogen were analyzed using gas chromatography (Quintron instrument company, Milwawkee, WI). A positive methane breath test was defined as a breath methane level ≥3 parts per million (ppm) as previously published [20,23]. Of these subjects, 15 non-methane and 5 methane positive subjects were consented to undergo an oral glucose tolerance test (OGTT).…”

Section: Methodsmentioning

confidence: 99%

“…In humans, breath methane has been associated with constipation: we found that subjects with IBS who have methane on breath test were almost universally constipated [20], and subsequent studies showed methane to be predictive of constipation among functional bowel disease patients [23] as well as in IBS and non-IBS subjects [24]. In a meta-analysis of 1277 IBS patients, we found that methane was associated with constipation, with a pooled OR=3.51 (CI=2.00-6.16) [25].…”

Section: Introductionmentioning

confidence: 99%

Methane-producing human subjects have higher serum glucose levels during oral glucose challenge than non-methane producers: a pilot study of the effects of enteric methanogens on glycemic regulation

Mathur¹,

Goyal²,

Kim³

et al. 2014

Res J Endocrinol Metab

Self Cite

View full text Add to dashboard Cite

Background: Recent studies support that intestinal microbes contribute to human disease, and enteric methanogens have been specifically linked to altered gut metabolism and weight gain. In this study, we tested whether methane on breath test (as a surrogate for colonization with the predominant methanogen Methanobrevibacter smithii) is associated with altered glucose tolerance in humans. Methods: Consecutive methane producing (methane ≥3ppm, N=5) and non-methane producing (methane <3ppm, N=15) subjects undergoing lactulose breath test at our center were recruited and subjected to a 75 g oral glucose tolerance test (OGTT). Results: The average age of methane-producing subjects was 48.8±10.0 vs. 37.7±12.1 for non-methane subjects (P=0.17). Methane and non-methane subjects also had comparable mean body mass index (BMI) (23.9±0.2 vs. 25.0±8.0 kg/m2; P=0.53) and baseline insulin resistance (HOMA-IR) (1.32±0.72 vs. 2.21±1.52; P=0.23). During 180 minutes post-glucose load, methane producers had greater serum glucose area-under-the-curve (AUC) (774.2±140.3 mg/dL) than non-methane subjects (585.5±128.3 mg/dL) (P=0.03), but similar insulin AUC (217.76±122.08 μU/mL vs. 215.37±75.02 μU/mL, respectively). Conclusions: Individuals with methane on breath test (reflecting higher colonization with enteric methanogens, predominantly M. smithii) may have impaired glucose tolerance when challenged with a high carbohydrate load, and may also have a higher susceptibility to hyperglycemia which appears to be independent of basal insulin resistance and BMI.

show abstract

“…spp. is increased in IBD patients (Chatterjee et al, 2007, Blais Lecours et al, 2014. With all these factors considered it led me to hypothesise that the abundance and profile of the methanogenic community within CKD…”

Section: Discussionmentioning

confidence: 99%

“…Irritable bowel syndrome (IBS) is categorised by episodes of diarrhoea (IBS-D), constipation (IBS-C), or both (IBS-M), and breath methane measurement of IBS patients has been reported to be greater in IBS-C and lower or not detectable in IBS-D patients (Chatterjee et al, 2007). It has been demonstrated in animal and human models that methane can influence the intestinal motor function, with an abundance of methane resulting in a slow intestinal transit time as a result of augmented small bowel contractile activity (Pimentel et al, 2006).…”

Section: Interactions Between Methanogenic Archaea and The Human Hostmentioning

confidence: 99%

Functional and comparative studies of members of the genus Methanosphaera, and their adaptations to the gut environment

Hoedt¹

View full text Add to dashboard Cite

The methanogenic archaea are responsible for maintaining an efficient scheme of fermentation in many environments and habitats, including the gastrointestinal tracts of animals and humans. The principal members of gut methanogenic communities are members of the Methanobrevibacter genus, with lesser numbers of Methanosphaera and Methanomassiliicoccus spp. Much of our understanding of gut methanogens has been produced using axenic cultures and genomic data for ~30 Methanobrevibacter spp. but the functional relevance of these other archaeal lineages to digestive function remains poorly understood. With this background, the goals of my PhD research are: i) to increase the biotic representation of the Methanosphaera genus through the recovery of axenic isolates from different environments; ii) characterise the metabolic properties of these isolates in terms of their methanogenic pathways and; iii) expand our functional understanding of this genus via reconstruction of "population genomes" from existing metagenomic datasets and comparative Mbp), synteny, and G:C content. However, the WGK6 genome was found to encode contiguous genes encoding putative alcohol-and aldehyde-dehydrogenases, which are absent from the Msp. stadtmanae genome. These two genes provide a plausible explanation for the ability of WGK6 to utilize ethanol for methanol reduction to methane. Furthermore, my in vitro studies suggest that ethanol supports a greater cell yield per mol of methane formed compared to hydrogen-dependent growth. Taken together, this expansion in metabolic versatility can explain the persistence of these archaea in the kangaroo foregut, and their abundance in these "low methane emitting" herbivores.Chapter Three describes the isolation of a hydrogen-dependent methylotrophic archaeon assigned to the Methanosphaera genus from bovine animals in northern Australia (strain BMS). The BMS genome was sequenced to closure and surprisingly, found to be substantially larger (2.9 Mbp) than the WGK6 and Msp. stadtmanae genomes (~1.8 Mbp). I then interrogated metagenomic datasets produced from human stool and ruminant digesta samples, to recover 7 "population genomes" assigned to the Methanosphaera genus, with 5 of these genomes also predicted to be large (> 2.1 Mbp). The Methanosphaera pan-genome consists of 5321 genes and 305 of these were assigned to the core-genome, which principally consists of the genes coordinating methanogenesis, anaerobic metabolism, and related functions. The whole genome phylogeny analysis of all genomes suggests a monophyletic origin for the genus Methanosphaera, with those isolates possessing smaller genomes being the most recently evolved lineages.Chapter Four investigates whether and how the composition of the methanogenic archaea in patients with chronic kidney disease is altered in response to synbiotic administration, as an intervention designed to mitigate uremic toxin production. The synbiotic intervention was found to have no measurable impact on the methanogen profiles of the patients, withMeth...

show abstract

The Degree of Breath Methane Production in IBS Correlates With the Severity of Constipation

Abstract: Methane on LBT is associated with constipation both subjectively and objectively. The degree of methane production on breath test appears related to the degree of constipation.

Cited by 189 publications

References 22 publications

The networks of human gut microbe–metabolite associations are different between health and irritable bowel syndrome

The networks of human gut microbe–metabolite associations are different between health and irritable bowel syndrome

Methane-producing human subjects have higher serum glucose levels during oral glucose challenge than non-methane producers: a pilot study of the effects of enteric methanogens on glycemic regulation

Functional and comparative studies of members of the genus Methanosphaera, and their adaptations to the gut environment

Contact Info

Product

Resources

About