The Degree of Hemagglutination on a Plastic Microfluidic Chip Using Impedimetric Detection

Samae, Mahdee; Chirasatitsin, Somyot

doi:10.1109/bmeicon47515.2019.8990253

Cited by 2 publications

(1 citation statement)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to the standard cell, which is normally characterized as a level 4+ strength of agglutination, this could be the cause of the complete red cell agglutination reaction plugging onto the pores of the filter paper, so that there is no outflow of incomplete reaction of red cells into the detection zone. However, previous work [26] has shown that the image processing technique was limited, as the strength of agglutination varied from level 0-4+. Another detection technique, such as impedimetric measurement, could be applied.…”

Section: Quantification Of Blood Agglutinationmentioning

confidence: 99%

Hemagglutination Detection with Paper–Plastic Hybrid Passive Microfluidic Chip

2021

Self Cite

View full text Add to dashboard Cite

Hemagglutination is a critical reaction that occurs when antigens expressed on red blood cells (RBCs) react with the antibodies used for blood typing. Even though blood typing devices have been introduced to the market, they continue to face several limitations in terms of observation by the eye alone, blood manipulation difficulties, and the need for large-scale equipment, particularly process automated machines. Thus, this study aimed to design, fabricate, and test a novel hybrid passive microfluidic chip made of filter paper and polymer using a cost-effective xurography manufacturing technique. This chip is referred to as the microfluidic paper–plastic hybrid passive device (PPHD). A passive PPHD does not require external sources, such as a syringe pump. It is composed of a paper-based component that contains dried antibodies within its porous paper and a polymer component that serves as the detection zone. A single blood sample was injected into the chip’s inlet, and classification was determined using the mean intensity image. The results indicated that embedded antibodies were capable of causing RBC agglutination without a saline washing step and that the results could be classified as obviously agglutination or nonagglutination for blood typing using both the naked eye and a mean intensity image. As a proof-of-concept, this study demonstrated efficiency in quantitative hemagglutination measurement within a passive PPHD for blood typing, which could be used to simplify blood biomarker analysis.

show abstract

Section: Quantification Of Blood Agglutinationmentioning

confidence: 99%