The Delivery of the Novel Drug ‘Halicin’ Using Electrospun Fibers for the Treatment of Pressure Ulcer against Pathogenic Bacteria

Abstract

Pressure ulcer or bedsore is a form of skin infection that commonly occurs with patients admitted to the hospital for an extended period of time, which might lead to severe complications in the absence of medical attention, resulting in infection either by drug-sensitive or drug-resistant bacteria. Halicin, a newly discovered drug effective against several bacterial strains, including multidrug-resistant bacteria, was investigated to reduce bacterial infection burden. This study aims to formulate halicin into electrospun fibers to be applied in bedsores as antibacterial dressing to assess its efficacy against gram-positive (Staphylococcus aureus) and gram-negative bacteria (Escherichia coli and Acinetobacter baumannii) by studying the minimum inhibitory concentration (MIC) and bacterial zone of inhibition assays. The diameters of inhibition growth zones were measured, and the results have shown that the drug-loaded fibers were able to inhibit the growth of bacteria compared to the halicin discs. The release profile of the drug-loaded fibers exhibited a complete release of the drug after 2 h. The results demonstrated that the drug-loaded fibers could successfully release the drug while retaining their biological activity and they may be used as a potential antimicrobial dressing for patients with pressure ulcers caused by multidrug resistant bacteria.

Fabrication and Characterization of Fast-Dissolving Films Containing Escitalopram/Quetiapine for the Treatment of Major Depressive Disorder

“…The spectrum showed two broad bands at 3650–3100 and at 3030–2790 cm −1 that were related to the O–H stretching, from adsorbed water, and the C–H stretching, respectively. This spectrum is consistent with the PVP IR spectrum of [ 33 , 35 , 44 , 47 , 55 ]. The stretching peaks of PVP at 1656 cm −1 and between 3650 and 3100 cm −1 were present in the PM, blank, and drug-loaded fibers IR spectra.…”

“…Consequently, a slower dissolution rate can be obtained, compared to lower MW PVP. Similar MW PVP was used by Rasekh et al and Aburayan et al for preparing indomethacin- and halicin-loaded fibers, respectively; the full drug release for both studies was reported to be over 30 min [ 55 , 61 ].…”

Formulation and Evaluation of Alcohol-Free Hand Sanitizer Gels to Prevent the Spread of Infections during Pandemics

“…The yeast C. albicans —ATCC 66,027 was obtained from ATCC. The bacterial and yeast suspensions, also known as inoculums, were all prepared in Mueller–Hinton broth by measuring 0.5 McFarland, according to [ 19 ]. All microorganisms were cultured on Mueller–Hinton agar medium and incubated at 37 °C overnight.…”

“…New infections, bacterial or viral, have often raised significant threats to public health across the globe. One of these hazardous pathogens is severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is renowned to cause coronavirus disease 2019 (COVID- 19) that was declared a global pandemic by the World Health Organization (WHO) at the beginning of 2020 [1]. After its discovery in Wuhan in December 2019, more than 150 million confirmed cases have occurred worldwide by April 2021.…”

Preparation and evaluation of benzalkonium chloride hand sanitizer as a potential alternative for alcohol-based hand gels

“…The bacterial strains that were tested included; Acinetobacter baumannii ( A. baumannii ) BAA 747, two strains of Escherichia coli ( E. coli ) ATCC 25,922 and a clinical isolate 1060, Klebsiella pneumoniae ( K. pneumoniae ) BAA 1705, two strains of Pseudomonas aeruginosa ( P. aeruginosa ) BAA 1744 and ATCC 27853, Staphylococcus aureus ( S. aureus ) ATCC 29213, Staphylococcus epidermidis ( S. epidermidis ) clinical isolate 5029, Staphylococcus hominis ( S. hominis ) clinical isolate 5028, Staphylococcus haemolyticus ( S. haemolyticus ) clinical isolate 5034, and Micrococcus luteus ( M. luteus ) environmental isolate SB 115. Bacterial inoculums were prepared in Mueller-Hinton broth by measuring 0.5 McFarland, as previously reported ( Aburayan et al, 2020 ).…”