The DELLA Proteins Interact with MYB21 and MYB24 to Regulate Filament Elongation in Arabidopsis

Abstract

Background: Gibberellin (GA) and jasmonate (JA) are two essential phytohormones for filament elongation in Arabidopsis . GA and JA trigger degradation of DELLAs and JASMONATE ZIM-domain (JAZ) proteins through SCF SLY1 and SCF COI1 separately to activate filament elongation. In JA pathway, JAZs interact with MYB21 and MYB24 to control filament elongation. However, little is known how DELLAs regulate filament elongation. Results: Here we showed that DELLAs interact with MYB21 and MYB24, and that R2R3 domains of MYB21 and MYB24 are responsible for interaction with DELLAs. Furthermore, we demonstrated that DELLA and JAZ proteins coordinately repress the transcriptional function of MYB21 and MYB24 to inhibit filament elongation . Conclusion: We discovered that DELLAs interact with MYB21 and MYB24, and that DELLAs and JAZs attenuate the transcriptional function of MYB21 and MYB24 to control filament elongation. This study reveals a novel cross-talk mechanism of GA and JA in the regulation of filament elongation in Arabidopsis . Keywords: Gibberellin, Jasmonate, MYB21, MYB24, Filament elongation

Potato virus X -delivered CRISPR activation programs lead to strong endogenous gene induction and transient metabolic reprogramming inNicotiana benthamiana

“…With the second criterium, we aimed to show the possibility to create distinctive MYB-specific metabolite fingerprints in leaves using PVX_VGR (Figure 3A). Highly homologous genes for all three TFs were earlier described as modifiers of the phenylpropanoid composition and/or of the volatile profiles in Arabidopsis, petunia and tomato, respectively (Verdonk et al 2005; Dal Cin et al 2011; Huang et al 2020). Very little is known, however, about their role in N. benthamiana.…”