The dentin–enamel junction—a natural, multilevel interface

Marshall, Sally J.; Balooch, M.; Habelitz, Stefan; Balooch, Guive; Gallagher, R. R.; Marshall, Grayson W.

doi:10.1016/s0955-2219(03)00301-7

Cited by 103 publications

(96 citation statements)

References 13 publications

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“…Mechanically, DEJ plays an important role in preventing crack propagation from enamel into dentine (Imbeni et al 2005). Hierarchically, DEJ has a three-level structure: 25-100 lm scallops with their convexities directed toward the dentin and concavities toward the enamel, 2-5 lm microscallops and a smaller scale structure (Marshall et al 2003). Therefore, high-resolution elastic modulus mapping has indicated that the DEJ is a band with a graded mechanical property rather than a discrete interface (Sui et al 2014).…”

Section: Teethmentioning

confidence: 99%

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Dorozhkin¹

2017

Morphologie

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Section: Teethmentioning

confidence: 99%

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Dorozhkin¹

2017

Morphologie

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“…Such natural HAp crystallites are understood to be crucial to the physiology and function of dental tissues [1]. In parallel, recent developments in synthetic biomineralization have demonstrated that synthetic HAp powders can play an important role for medical applications, especially for the replacement or treatment of dental tissues [2].…”

Section: Introductionmentioning

confidence: 99%

In situ X-ray scattering evaluation of heat-induced ultrastructural changes in dental tissues and synthetic hydroxyapatite

Sui

Sandholzer

Lunt

et al. 2014

J. R. Soc. Interface.

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Human dental tissues consist of inorganic constituents (mainly crystallites of hydroxyapatite, HAp) and organic matrix. In addition, synthetic HAp powders are frequently used in medical and chemical applications. Insights into the ultrastructural alterations of skeletal hard tissues exposed to thermal treatment are crucial for the estimation of temperature of exposure in forensic and archaeological studies. However, at present, only limited data exist on the heat-induced structural alterations of human dental tissues. In this paper, advanced nondestructive small-and wide angle X-ray scattering (SAXS/WAXS) synchrotron techniques were used to investigate the in situ ultrastructural alterations in thermally treated human dental tissues and synthetic HAp powders. The crystallographic properties were probed by WAXS, whereas HAp grain size distribution changes were evaluated by SAXS. The results demonstrate the important role of the organic matrix that binds together the HAp crystallites in responding to heat exposure. This is highlighted by the difference in the thermal behaviour between human dental tissues and synthetic HAp powders. The X-ray analysis results are supported by thermogravimetric analysis. The results concerning the HAp crystalline architecture in natural and synthetic HAp powders provide a reliable basis for deducing the heating history for dental tissues in the forensic and archaeological context, and the foundation for further development and optimization of biomimetic material design.

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“…Despite a lack of structural and compositional information about the DEJ and interfacial region (Marshall et al, 2003), its mechanical importance to the stability of teeth has been amply demonstrated. Specifically, the DEJ and associated inner enamel are known to inhibit crack propagation, exhibit higher fracture toughness (Imbeni et al, 2005), and rarely undergo catastrophic mechanical failure despite a lifetime of masticatory and parafunctional loading (Paine et al, 2001).…”

mentioning

confidence: 99%

Type IV Collagen is a Novel DEJ Biomarker that is Reduced by Radiotherapy

McGuire¹,

Gorski

Dusevich³

et al. 2014

J Dent Res

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The dental basement membrane (BM) is composed of collagen types IV, VI, VII, and XVII, fibronectin, and laminin and plays an inductive role in epithelial-mesenchymal interactions during tooth development. The BM is degraded and removed during later-stage tooth morphogenesis; however, its original position defines the location of the dentinenamel junction (DEJ) in mature teeth. We recently demonstrated that type VII collagen is a novel component of the inner enamel organic matrix layer contiguous with the DEJ. Since it is frequently co-expressed with and forms functional complexes with type VII collagen, we hypothesized that type IV collagen should also be localized to the DEJ in mature human teeth. To identify collagen IV, we first evaluated defect-free erupted teeth from various donors. To investigate a possible stabilizing role, we also evaluated extracted teeth exposed to high-dose radiotherapy -teeth that manifest post-radiotherapy DEJ instability. We now show that type IV collagen is a component within the morphological DEJ of posterior and anterior teeth from individuals aged 18 to 80 yr. Confocal microscopy revealed that immunostained type IV collagen was restricted to the 5-to 10-µm-wide optical DEJ, while collagenase treatment or previous in vivo tooth-level exposure to > 60 Gray irradiation severely reduced immunoreactivity. This assignment was confirmed by Western blotting with whole-tooth crown and enamel extracts. Without reduction, type IV collagen contained macromolecular α-chains of 225 and 250 kDa. Compositionally, our results identify type IV collagen as the first macromolecular biomarker of the morphological DEJ of mature teeth. Given its network structure and propensity to stabilize the dermal-epidermal junction, we propose that a collagen-IV-enriched DEJ may, in part, explain its wellknown fracture toughness, crack propagation resistance, and stability. In contrast, loss of type IV collagen may represent a biochemical rationale for the DEJ instability observed following oral cancer radiotherapy.

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The dentin–enamel junction—a natural, multilevel interface

Cited by 103 publications

References 13 publications

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

In situ X-ray scattering evaluation of heat-induced ultrastructural changes in dental tissues and synthetic hydroxyapatite

Type IV Collagen is a Novel DEJ Biomarker that is Reduced by Radiotherapy

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