The Denture-Associated Oral Microbiome in Health and Stomatitis

Shi, Baochen; Wu, Tingxi; McLean, Jeffrey S.; Edlund, Anna; Young, Youngik; He, Xuesong; Lv, Hongyang; Zhou, Xuedong; Shi, Wenyuan; Li, Huiying; Lux, Renate

doi:10.1128/msphere.00215-16

Cited by 58 publications

(54 citation statements)

References 61 publications

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“…Our data indicates that the alpha diversity of the salivary microbiome in the control groups was significantly higher in comparison to those suffering from poor oral health, which is similar to previously reported results [64]. Our data show that usage of denture is positively correlated with age and is closely linked with the oral health status [65]. In this study, we show that using denture reduces the diversity of the salivary microbiome with an enrichment of both Proteobacteria and Actinobacteria phyla while Streptococcus and Neisseria were enriched at the genus levels.…”

Section: Discussionsupporting

confidence: 91%

Profiling the Salivary microbiome of the Qatari population

et al. 2020

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Background: The role of the human microbiome in human health and disease has been studied in various body sites. However, compared to the gut microbiome, where most of the research focus is, the salivary microbiome still bears a vast amount of information that needs to be revealed. This study aims to characterize the salivary microbiome composition in the Qatari population, and to explore specific microbial signatures that can be associated with various lifestyles and different oral conditions. Materials and methods: We characterized the salivary microbiome of 997 Qatari adults using high-throughput sequencing of the V1-V3 region of the 16S rRNA gene. Results: In this study, we have characterized the salivary microbiome of 997 Qatari participants. Our data show that Bacteroidetes, Firmicutes, Actinobacteria and Proteobacteria are the common phyla isolated from the saliva samples, with Bacteroidetes being the most predominant phylum. Bacteroidetes was also more predominant in males versus females in the study cohort, although differences in the microbial diversity were not statistically significant. We also show that, a lower diversity of the salivary microbiome is observed in the elderly participants, with Prevotella and Treponema being the most significant genera. In participants with oral conditions such as mouth ulcers, bleeding or painful gum, our data show that Prevotella and Capnocytophaga are the most dominant genera as compared to the controls. Similar patterns were observed in participants with various smoking habits as compared to the non-smoking participants. Our data show that Streptococcus and Neisseria are more dominant among denture users, as compared to the non-denture users. Our data also show that, abnormal oral conditions are associated with a reduced microbial diversity and microbial richness. Moreover, in this study we show that frequent coffee drinkers have higher microbial diversity compared to the non-drinkers, indicating that coffee may cause changes to the salivary microbiome. Furthermore, tea drinkers show higher microbial richness as compared to the non-tea drinkers. Conclusion: This is the first study to assess the salivary microbiome in an Arab population, and one of the largest population-based studies aiming to the characterize the salivary microbiome composition and its association with age, oral health, denture use, smoking and coffee-tea consumption.

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Section: Discussionsupporting

confidence: 91%

Profiling the Salivary microbiome of the Qatari population

et al. 2020

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“…Alterations of the oral cavity condition in reaction to an intraoral prosthesis may cause lack of saliva accessibility and tongue-associated mechanical cleansing [24]. Furthermore, dentures can harbor mixed kinds of [25,26]. 18 kinds of bacteria were isolated from denture plaque that can induce respiratory infection in elderly [19,27].…”

Section: Discussionmentioning

confidence: 99%

PCR Detection of HHV8 DNA in the Saliva of Removable Denture Wearers Compared to Dentate Cases in Shiraz, South of Iran

Derafshi

Ghapanchi

Rezazadeh

et al. 2020

BioMed Research International

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Background and aim. Removable dentures may act as reservoir for pathogens that can cause serious infections. Human herpesvirus 8 (HHV8) is an oncogenic virus that was found in the saliva more than genital secretions. Thus, this study was designed to evaluate HHV8 in the saliva of patients with removable dentures. Method and material. In this cross-sectional study from March-July 2019, saliva samples were collected from 50 denture wearers as a case group and 50 ages and gender matched dentate subjects as a control. Whole-mouth saliva, parotid saliva, buccal, and palatal exfoliates were collected and processed for HHV8-DNA amplification by nested PCR. A confirmatory real-time PCR was also performed in order to prevent false result. Result. In the denture wearers, HHV8 DNA was detected in 11 cases. Two of the controls amplified HHV8 DNA. Fisher’s exact test demonstrates a significant difference between virus infection and using removable dentures (p=0.015). Conclusion. Our findings suggested that HHV8 detection could be associated with use of denture.

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“…Ahn et al in a study examining saliva in dentate subjects showed the 10 most common genera in the oral microbiome were Streptococcus, Veillonella, Leptotrichia, Prevotella, Haemophilus, Capnocytophaga, Rothia, Gamella, Neisseria, and Lactococcus , a result supported by other similar studies (Ahn, Chen, & Hayes, ; Bik et al, ; Keijser et al, ). Others have examined the oral microbiome of edentulous patients, though this was done consistently with dentures in place (Marsh, Percival, & Challacombe, ; O'Donnell et al, ; Sachdeo, Haffajee, & Socransky, ; Shi et al, ). An early study showed that denture wearers produced saliva with more abundance of Lactobacilli, Staphylococci, Streptococci , and yeast than dentate subjects (Marsh et al, ).…”

Section: Introductionmentioning

confidence: 99%

Diversity of the oral microbiome between dentate and edentulous individuals

et al. 2019

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Background Measurement of saliva microbes is promoted as a way to detect oral and systemic disease, yet there is a multitude of factors that affect the oral microbiome. The salivary microbiome is influenced by oral biofilm of shedding (epithelial) and non‐shedding (tooth) surfaces. Methods To gauge the ability of salivary microbial analytics to distinguish between edentulous and dentate oral conditions, we looked for differences in the saliva microbiome of subjects with and without teeth. Fifty‐two dentate and 49 edentulous subjects provided stimulated saliva samples. 16S rRNA gene sequencing, QIIME‐based data processing, and statistical analysis were done using several different analytical approaches to detect differences in the salivary microbiome between the two groups. Results Bacteria diversity was lower in the edentulous group. Remarkably, all 31 of the most significant differences in taxa were deficits that occur in the edentulous group. As one might expect many of these taxa are attributed to dental plaque and gingival sulcus associated bacteria. Conclusion In sum, the measurement of 16S rRNA genes in the bacteria of the saliva can be used to reproducibly measure differences in the oral microbiome that occur with edentulism, mainly the lack of tooth and tooth‐related structures.

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The Denture-Associated Oral Microbiome in Health and Stomatitis

Cited by 58 publications

References 61 publications

Profiling the Salivary microbiome of the Qatari population

Profiling the Salivary microbiome of the Qatari population

PCR Detection of HHV8 DNA in the Saliva of Removable Denture Wearers Compared to Dentate Cases in Shiraz, South of Iran

Diversity of the oral microbiome between dentate and edentulous individuals

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