The Description and Evaluation of a Competitive Protein-Binding Radioassay of Plasma Progesterone in Pregnancy

Luisi, M; Levanti, Salvatore; Franchi, F

doi:10.1016/s0015-0282(16)38343-1

Cited by 5 publications

(3 citation statements)

References 24 publications

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“…Nevertheless, it must be mentioned that high concentrations in plasma of interfering steroids, i.e. 17a-hydroxyprogesterone and 20a-dihydroprogesterone, may influence the measurement of progesterone (Johansson, 1969;Luisi, Levanti & Franchi, 1971). No information is available concerning the different steroids present in peripheral plasma in pregnant mink.…”

Section: Progesterone Assaymentioning

confidence: 99%

The Progesterone Concentrations in the Peripheral Plasma of the Mink (Mustela Vison) During Pregnancy

Møller¹

1973

Journal of Endocrinology

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In mink bitches mated once, the progesterone levels in plasma remained at the basal level (< 8 ng/ml, i.e. comparable with the progesterone levels in anoestrous mink) during the first days after coitus. The duration of this lag period varied from 10 to about 20 days, and was found to correspond with the length of gestation. However, in general a gradual increase was observed beginning about 40 days before parturition with a maximum plasma progesterone concentration (63-105 ng/ml) from 10 to 25 days later on. After the peak value had been attained the plasma progesterone concentration declined gradually to the day of parturition and was low ( < 10 ng/ml) at parturition and afterwards. The progesterone values and profiles obtained from females mated twice corresponded with those obtained from females mated once. The increase in progesterone, however, began a few days after the second mating.The developmental stages of the foetuses corresponded clearly with the progesterone profile.In three mated females which failed to give birth a slow increase in progesterone concentration was observed beginning about 15 days post coitum. In these bitches the progesterone concentration reached a plateau (30-45 ng/ml) between day 30 and day 40 post coitum, after which a slow decline was observed. The period when the progesterone concentrations were increased above the basal level lasted about 40 days even in the females which did not give birth.

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Section: Progesterone Assaymentioning

confidence: 99%

The Progesterone Concentrations in the Peripheral Plasma of the Mink (Mustela Vison) During Pregnancy

Møller¹

1973

Journal of Endocrinology

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“…Therefore no evidence emerged, as a result of this research, that high affinity progesterone-binding protein was Plasma progesterone in the blue fox 433 present in pregnant vixens, as has been reported in pregnant guinea-pigs (Challis, Heap & Illingworth, 1971). The ability of many steroids to displace corticosterone from corticosteroid-binding globulin is well known (Johansson, 1969;Edqvist, Ekman, Gustafsson & Aström, 1970;Challis et al 1971;Luisi, Levanti & Franchi, 1971). Therefore any high concentrations of interfering steroids in the vixen plasma, e.g.…”

Section: Preparation Of Standard Curve and Extraction Of The Plasma Smentioning

confidence: 99%

Progesterone Concentrations in the Peripheral Plasma of the Blue Fox (Alopex Lagopus) During Pregnancy and the Oestrous Cycle

Møller¹

1973

Journal of Endocrinology

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A competitive protein-binding assay was evaluated and employed in the measurement of progesterone in serial plasma samples obtained from pregnant, non-pregnant and non-receptive blue-fox vixens.At the beginning of the period of pro-oestrous vulval swelling, the plasma progesterone concentration was always found to be below 2 ng/ml. Towards the end of pro-oestrus and during the period of oestrus (sexual receptivity) the concentration increased rather quickly. On the 3rd and the 4th days of oestrus, i.e. when most of the vixens were mated, the plasma progesterone concentrations were about 30 ng/ml (range: 18\p=n-\36 n g / ml ) . During gestation (53 or 54 days) the plasma progesterone level rose very steeply and attained a maximum plateau (> 80 ng/ml) between days 5 and 8 post coitum, remained high until about day 20, but thereafter fell rather quickly to below 30 ng/ml between days 35 and 40. Later on the progesterone level fell gradually to below 5 ng/ml at the day of parturition, and, 4 days post partum and later, the levels were below the limit of the sensitivity of the assay used (< 2 ng/ml).The progesterone values and profiles obtained from the non-pregnant vixens (mated vixens which failed to whelp) corresponded clearly to those obtained in the pregnant vixens. Indeed, even in two non-receptive vixens (unwilling to mate during the mating season) typical progesterone profiles were obtained during a period of about 70 days.

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“…The water was deionized, then distilled twice. Florisil (60-100 mesh) obtained from BDH, was washed 5 times with distilled water and the fine particles were decanted each time; it was then dried at 110°C for 12 h. For the purification of the plasma extracts, horizontal thin-layer chromatography (HTLC) was carried out using 20 x 20-cm glass plates stratified with silica gel HF25< (Merck, Germany) and the BN-Kammer apparatus (Desaga, Germany) as previously reported [6]. Before use, the plates were washed twice with methanol, once with diethyl ether and then stored in airtight containers.…”

Section: Methodsmentioning

confidence: 99%

The Description and Evaluation of a Competitive Protein-Binding Radioassay of 17α-Hydroxyprogesterone in Human Plasma

Luisi¹,

Franchi²,

Simonini³

et al. 1973

Horm Res

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A sensitive and precise method, based on the technique of competitive protein binding, has been developed for the determination of 17α-hydroxyprogesterone in human plasma. The method consists of extraction with ether, HTLC to purify the plasma extracts and CBG radioassay. The reliability criteria of the complete procedure are presented. The method is suitable for application to peripheral plasma levels at all stages of the menstrual cycle, in men and in cases of endocrine diseases. The plasma values of 17α-hydroxyprogesterone in men and during the follicular and luteal phases of the menstrual cycle were 85.0 ± 30.6, 37.6 ± 14.3 and 169.0 ± 39.5 (SD) ng/100 ml, respectively. Results on patients with endocrine diseases are reported.

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The Description and Evaluation of a Competitive Protein-Binding Radioassay of Plasma Progesterone in Pregnancy

Cited by 5 publications

References 24 publications

The Progesterone Concentrations in the Peripheral Plasma of the Mink (Mustela Vison) During Pregnancy

The Progesterone Concentrations in the Peripheral Plasma of the Mink (Mustela Vison) During Pregnancy

Progesterone Concentrations in the Peripheral Plasma of the Blue Fox (Alopex Lagopus) During Pregnancy and the Oestrous Cycle

The Description and Evaluation of a Competitive Protein-Binding Radioassay of 17α-Hydroxyprogesterone in Human Plasma

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The Description and Evaluation of a Competitive Protein-Binding Radioassay of Plasma Progesterone in Pregnancy

Cited by 5 publications

References 24 publications

The Progesterone Concentrations in the Peripheral Plasma of the Mink (Mustela Vison) During Pregnancy

The Progesterone Concentrations in the Peripheral Plasma of the Mink (Mustela Vison) During Pregnancy

Progesterone Concentrations in the Peripheral Plasma of the Blue Fox (Alopex Lagopus) During Pregnancy and the Oestrous Cycle

The Description and Evaluation of a Competitive Protein-Binding Radioassay of 17&alpha;-Hydroxyprogesterone in Human Plasma

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The Description and Evaluation of a Competitive Protein-Binding Radioassay of 17α-Hydroxyprogesterone in Human Plasma