The design and development of short peptide-based novel smart materials to prevent fouling by the formation of non-toxic and biocompatible coatings

Arul, Amutha; Sivagnanam, Subramaniyam; Dey, Ananta; Mukherjee, Oindrilla; Ghosh, Soumyajit; Das, Priyadip

doi:10.1039/c9ra10018k

Cited by 20 publications

(18 citation statements)

References 81 publications

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“…These chemical groups contain aspartic acid as an anchoring moiety that exhibited antifouling activity and self-assembling coating. Likewise, the dicarboxylate groups revealed antifouling activity and generate a non-toxic biocompatibility on the desired surface (36). The H-P extract that contains several dicarboxylate groups and tagged with an aspartic acid group showed an obvious regulation of the tumorigenesis NF-kB effector, Raf-1 activity, and conjugated LC3B protein, the key mediator for the maturation of autophagosomes.…”

Section: Discussionmentioning

confidence: 99%

Effective Targeting of Raf-1 and Its Associated Autophagy by Novel Extracted Peptide for Treating Breast Cancer Cells

El-Fadl

Hagag²,

El‐Shafei

et al. 2021

Front. Oncol.

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Breast cancer is one of the most common causes of death in women worldwide and has harmful influence on their psychological state during therapy. Multikinase inhibitors have become effective drugs for treating a variety of cancer diseases such as breast cancer. A purified short peptide (H-P) was isolated from the natural honey and tested for its potential regulatory role in breast cancer cells compared with the effectiveness of the anticancer drug, Sorafenib (SOR), using MCF-7, EFM-19, and MCF-10A cell lines. Furthermore, we investigated the direct connection between Raf-1 activation and cellular autophagy as potential targets of SOR and H-P extract using RNA interference. Interestingly, the treatment with H-P showed competitive regulation of phosphorylated Raf-1, MEK1/2, and matched autophagy-related LC3B without any detectable toxic effects in the non-tumorigenic epithelial cells. Unlike SOR, the regulation of Raf-1 protein and autophagic machinery by the novel H-P extract showed neglected levels of the released proinflammatory cytokine. This regulation of cytokine secretion by H-P resulted in decreasing the expression level of the transcription factor nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) in treated cells. Moreover, the transfection of MCF-7 cells with small interference RNA (siRNA) antagonist Raf-1 expression markedly reduced the expression of LC3B, while it increased the expression of NF-kB1 and NF-kB2, indicating the potential cross-link between Raf-1, autophagy, and NF-kB effector. Collectively, these findings suggest that H-P-mediated Raf-1, MEK1/2, LC3B, and NF-kB provide a novel and efficacious multikinase inhibitor for treating breast cancer without detectable cytotoxic effects.

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Section: Discussionmentioning

confidence: 99%

Effective Targeting of Raf-1 and Its Associated Autophagy by Novel Extracted Peptide for Treating Breast Cancer Cells

El-Fadl

Hagag²,

El‐Shafei

et al. 2021

Front. Oncol.

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“…Peptides attract attention not only for their antibiotic activity [1][2][3] but also for applications in nanotechnology, as molecular sensors or logical elements, or as biomaterials for purifying water from environmental toxins [4][5][6][7][8]. Peptides are often able to provide a selective response to external stimuli, such as the presence of a specific analyte [9][10][11][12][13][14][15][16][17]. One of the most common reactions of peptides to an external stimulus is to undergo a conformational change that might be reversible [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

A Peptide-Based Trap for Metal Ions Studied by Electron Paramagnetic Resonance

et al. 2022

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Peptide-based materials provide a versatile platform for sensing and ion sequestration since peptides are endowed with stimuli-responsive properties. The mechanism of molecular sensing is often based on peptide structural changes (or switching), caused by the binding of the target molecule. One scope of sensing applications is the selection of a specific analyte, which may be achieved by adjusting the structure of the peptide binding site. Therefore, exact knowledge of peptide properties and 3D-structure in the ‘switched’ state is desirable for tuning the detection and for further molecular construction. Hence, here we demonstrate the performance of Electron Paramagnetic Resonance (EPR) spectroscopy in the identification of metal ion binding by the antimicrobial peptide trichogin GA IV. Na(I), Ca(II), and Cu(II) ions were probed as analytes to evaluate the impact of coordination number, ionic radii, and charge. Conclusions drawn by EPR are in line with literature data, where other spectroscopic techniques were exploited to study peptide-ion interactions for trichogin GA IV, and the structural switch from an extended helix to a hairpin structure, wrapped around the metal ion upon binding of divalent cations was proposed.

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“…Such films are used for most prototypes of biomolecular nanodevices [ 8 , 9 , 10 , 11 ] in electronics, optics, and biotechnology [ 12 , 13 ]. Electronic elements, such as field-effect transistors [ 14 , 15 , 16 ], solar batteries [ 17 ], sensors [ 18 ], and biocompatible electrodes, have already been developed and are now successfully functioning [ 19 , 20 , 21 , 22 , 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Aggregation Properties of Albumin in Interacting with Magnetic Fluids

Velichko

Nepomnyashchaya

Baranov

et al. 2021

IJMS

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In this study, interactions of Fe3O4 magnetic nanoparticles with serum albumin biomolecules in aqueous solutions were considered. The studies were conducted with the laser correlation spectroscopy and optical analysis of dehydrated films. It was shown that the addition of magnetite to an albumin solution at low concentrations of up to 10−6 g/L led to the formation of aggregates with sizes of up to 300 nm in the liquid phase and an increase in the number of spiral structures in the dehydrated films, which indicated an increase in their stability. With a further increase in the magnetite concentration in the solution (from 10−4 g/L), the magnetic particles stuck together and to albumin, thus forming aggregates with sizes larger than 1000 nm. At the same time, the formation of morphological structures in molecular films was disturbed, and a characteristic decrease in their stability occurred. Most stable films were formed at low concentrations of magnetic nanoparticles (less than 10−4 g/L) when small albumin–magnetic nanoparticle aggregates were formed. These results are important for characterizing the interaction processes of biomolecules with magnetic nanoparticles and can be useful for predicting the stability of biomolecular films with the inclusion of magnetite particles.

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The design and development of short peptide-based novel smart materials to prevent fouling by the formation of non-toxic and biocompatible coatings

Abstract: Two newly synthesized peptides, PA1 and PA2 with fluorinated aromatic unit and dicarboxylate group as an anchoring moiety able to form a non-toxic, stable, coating by a simple drop-coating method, improving the antifouling property of the surface.

Cited by 20 publications

References 81 publications

Effective Targeting of Raf-1 and Its Associated Autophagy by Novel Extracted Peptide for Treating Breast Cancer Cells

Effective Targeting of Raf-1 and Its Associated Autophagy by Novel Extracted Peptide for Treating Breast Cancer Cells

A Peptide-Based Trap for Metal Ions Studied by Electron Paramagnetic Resonance

Aggregation Properties of Albumin in Interacting with Magnetic Fluids

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