The Design of an Electromagnetic Rewarming System for Cryopreserved Tissue

Marsland, T.P.

doi:10.1007/978-1-4684-5469-7_17

Cited by 1 publication

(1 citation statement)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Early attempts to use this approach (Halasz et al 1967, Dietzman et al 1973, Guttman et al 1977 were made with conventional microwave ovens operating at 2450 MHz, but without success (Pegg et al 1978). However, Marsland showed that the optimum frequency range for dielectric heating was 300-1000 MHz (Marsland et al 1987) and this led Evans's group to design a power source and resonant applicator operating at 434 MHz (Marsland 1987, Evans et al 1992. This equipment was later refined by Robinson and Pegg (1999), and has been used for the studies described in this paper.…”

Section: Introductionmentioning

confidence: 99%

Electromagnetic re-warming of cryopreserved tissues: effect of choice of cryoprotectant and sample shape on uniformity of heating

et al. 2002

View full text Add to dashboard Cite

A method that has been proposed for the cryopreservation of tissues and organs is to add a cryoprotective agent (CPA) in sufficient concentration to allow vitrification, and to use rapid electromagnetic heating to prevent the formation of ice crystals during the re-warming. We have compared the physical and biological properties of four CPAs, measuring the speed and uniformity of heating in a 36 mm sphere placed in a 434 MHz applicator, and the toxicity to ECV304 endothelial cells. Ethanediol and dimethyl sulfoxide were found to be suitable for rapid, uniform heating but toxic to the endothelial cells at vitrifying concentrations. Butane-2,3-diol was less toxic, but the heating patterns were unacceptably non-uniform. Propane-1,2-diol was not significantly more toxic than butane-2,3-diol, and did allow uniform heating. It is therefore the best choice of CPA for the vitrification of tissues. We have shown that the uniformity of heating correlates with the dielectric properties of the perfusate. Furthermore, we have shown that uniform heating is feasible in non-spherical samples provided they are approximately ellipsoidal.

show abstract