This paper proposes an electro-hydraulic vibration control system based on the single-neuron PID algorithm, which improves the operating frequency of the electro-hydraulic fatigue testing machine and the control accuracy of the load force. Through mathematical modeling of the electro-hydraulic vibration system (EVS), a MATLAB/Simulink simulation, and experimental testing, this study systematically analyzes the output waveform of the EVS as well as the closed-loop situation of load force amplitude and offset under the action of the single-neuron PID algorithm. The results show that: the EVS with a 2D vibration valve as the core, which can control the movement of the spool in the two-degrees-of-freedom direction, can realize the output of an approximate sinusoidal load force waveform from 0 to 800 Hz. The system controlled by the single-neuron PID algorithm is less complex to operate than the traditional PID algorithm. It also has a short rise time for the output load force amplitude curve and a maximum control error of only 1.2%. Furthermore, it exhibits a rapid closed-loop response to the load force offset. The range variability of the load force is measured to be 1.43%. A new scheme for the design of EVS is provided in this study, which broadens the application range of electro-hydraulic fatigue testing machines.