The desoxyribosenucleic acid (DNA) content in spermatozoa of fertile and infertile human males

Leuchtenberger, Cecilie; Schradér, Franz; Weir, David R.; Gentile, Doreen P.

doi:10.1007/bf01259931

Cited by 57 publications

(18 citation statements)

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“…Thompson et al (28) also studied sperm head dimensions and showed that sperm that bind to the zona pellucida have different head dimensions than sperm that do not. Larger head dimensions have been associated with either a higher or a lower DNA content, oligozoospermia, and subfertility (19,20,35). Patient 22 in our study had large heads and was also oligozoospermic (9.6 × 106).…”

Section: Discussionmentioning

confidence: 99%

“…The fertilizing potential of semen samples has been shown to be related to sperm concentration, total sperm count, progressive motile sperm concentration, and normal sperm morphology, which are routinely measured in semen analysis prior to IVF (17,18); sperm head dimensions (19,20); the absence of histones in mature sperm nuclei (14,21,22); and the presence of double-stranded DNA (23,24). Little attention has been given to P1 and P2 levels, the correlation with in vitro fertilization (IVF) rates, and the characteristics of mature sperm.…”

Section: Introductionmentioning

confidence: 99%

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Human protamines and male infertility

Khara

Vlad

Griffiths

et al. 1997

J Assist Reprod Genet

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Human protamines and male infertility

Khara

Vlad

Griffiths

et al. 1997

J Assist Reprod Genet

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“…Reduced fertility of some mammalian spermatozoa has been attributed to a lower DNA content (Leuchtenberger, Weir, Schrader & Murmanis, 1955;Leuchtenberger, Murmanis, Murmanis, Ito & Weir, 1956;Salisbury & Flerchinger, 1961) or to ageing of the sperm cell (Segina & Norman, 1964;Anand, Hoekstra & First, 1967). It was of interest, therefore, to determine the activity and properties of the enzymes most probably concerned with the degradation of DNA in spermatozoa.…”

Section: Introductionmentioning

confidence: 98%

Deoxyribonuclease Activity in Semen

Pj¹

1968

Reproduction

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The methyl green assay method has been used to determine the activity of DNase I and DNase II in ram, bull, human, dog and rabbit seminal plasma. The activity of DNase II was higher except in the ram. The DNase I activity of ram seminal plasma was inhibited by low concentrations of citrate, zinc and cetyltrimethylammonium bromide ; this detergent also caused a reduction of DNase II activity. There was a stimulation of DNase I activity by 10 mm-citrate but not by concentrations less than 2 mm. Addition of antibiotics or shaking of ram seminal plasma with toluene did not affect the activity of either DNase. After deep freezing of ram semen there was a decrease of DNase I activity in the plasma and an increase of DNase I and II activity in extracts obtained from disintegrated spermatozoa. No significant change in DNase activity could be detected in the plasma of ram semen which had previously been subjected to cold shock.

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“…Introduction chemical methods , by single cell cytophotometry as Feulgen- (Leuchtenberger et al -1953) or ultraviolet-cytophotometry (Meyhofer -1963), or by fluorescence flow cytophotometry (Sprenger et al -1971, van Dilla et al -1977Zante et al -1977). Regarding the mean DNA-content, the resulting data from infertile subjects were often contradictory.…”

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confidence: 99%

Deoxyribonucleic Acid (DNA) Content of Morphologically Different Sperm Types from Normal and Subfertile Human Males*

et al. 2009

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Zusammenfassung Desoxyribonukleinsäure‐Gehalt morphologisch unterschiedlicher Spermatozoentypen bei normalen und subfertilen Männern 20 männliche Patienten (G2), bei denen man eine idiopathische Oligozoospermie festgestellt hatte, und 10 normale, fertile Männer (G***1) wurden untersucht. Der DNA‐Gehalt von 50 nach Feulgen gefärbten Spermienköpfen pro Person wurde mit Hilfe eines Einzelzellphotometers gemessen. In einem zweiten Arbeitsgang wurde die Fläche, der Umfang, die Länge und die Breite der gleichen Spermienköpfe mit Hilfe eines halbautomatischen Bildanalyse‐gerätes gemessen. Bei einem Vergleich zwischen G1 und G2 unter Berücksichtigung von nur morphologisch normalen Köpfen ist der mittlere DNA‐Gehalt nur leicht reduziert in G2. Ganz im Gegen‐teil dazu ist die DNA‐Variation stark erhöht in G2. Unglücklicherweise, zeigen auch morphologisch defekte Köpfe eine erhöhte DNA‐Variation. Bei einer Bestimmung der DNA‐Variation in einem Samen mit vielen morphologisch defekten Köpfen ist deshalb eine gemeinsame Bestimmung von DNA‐Gehalt und Kopfmorphologie notwendig. Dies kann nur mit Hilfe der Einzelzellzytophotometrie erreicht werden. Auf dieses Problem bezogen ist deshalb die Einzelzellzytophotometrie der Durchflußzytophotometrie überlegen. Viele Sper‐mienköpfe mit stark abnormalem DNA‐Gehalt zeigen keine abnormen Morphologiemerk‐male. Diese subzellulären Veränderungen können nur mit einer DNA‐Bestimmung aufge‐zeigt werden.

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The desoxyribosenucleic acid (DNA) content in spermatozoa of fertile and infertile human males

Cited by 57 publications

References 30 publications

Human protamines and male infertility

Human protamines and male infertility

Deoxyribonuclease Activity in Semen

Deoxyribonucleic Acid (DNA) Content of Morphologically Different Sperm Types from Normal and Subfertile Human Males*

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