The detailed analysis of the changes of murine dendritic cells (DCs) induced by thymic peptide

Hu, Xiaofang; Zheng, Wei; Wang, Lu; Wan, Nan Chi; Wang, Bing; Li, Weiwei; Hua, Hui; Hu, Xu; Shan, Fengping

doi:10.4161/hv.20579

Cited by 12 publications

(11 citation statements)

References 22 publications

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“…During infancy, and in response to allergens, atopy-related type 2 T-helper cells (Th2) responses predominate [37,38]. Exposure to microbiota or immunomodulatory compounds can shift the balance from an atopic Th2 response to a nonatopic type 1 (Th1) response via the activation of DCs and subsequent regulatory T-cell activity [ 35,39,40,41,42,43].…”

Section: The Gut-lung Immune Axis: Infection and Inflammationmentioning

confidence: 99%

Immunoactive preparations and regulatory responses in the respiratory tract: potential for clinical application in chronic inflammatory airway diseases

Feleszko

Rossi

Krenke

et al. 2020

Expert Review of Respiratory Medicine

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Introduction: The prevalence of chronic inflammatory airway diseases is rising. Their treatment with corticosteroids increases infection risk, while overuse of antimicrobial agents may increase morbidity and antimicrobial resistance. Nonspecific immunomodulatory compounds alter immune responses to both infectious and atopic challenges. These compounds may offer an alternative approach for symptom reduction and prophylaxis against both infections and exacerbations in chronic inflammatory airway disease. Areas covered: We assessed the available data on the efficacy of nonspecific immunomodulators including bacterial lysates, synthetic compounds, and vaccines in chronic rhinosinusitis (CRS); allergic and non-allergic rhinitis; chronic obstructive pulmonary disease (COPD), and asthma. A search of PubMed was carried out using the 'Clinical Trials' filter for each condition and immunomodulatory product detailed below, where available, data from meta-analyses were reported. Expert opinion: Pre-clinical data has revealed a coherent mechanistic path of action for oral immunomodulators on the respiratory immune system, principally via the gut-lung immune axis. In patients with asthma, allergic rhinitis, CRS, and COPD immunomodulatory therapy reduces symptoms, exacerbations, hospitalizations, and drug consumption. However, data are heterogeneous, and study quality remains limited. A lack of high-quality recent trials remains the major unmet research need in the field. ARTICLE HISTORY

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Section: The Gut-lung Immune Axis: Infection and Inflammationmentioning

confidence: 99%

Immunoactive preparations and regulatory responses in the respiratory tract: potential for clinical application in chronic inflammatory airway diseases

Feleszko

Rossi

Krenke

et al. 2020

Expert Review of Respiratory Medicine

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“…Briefly, BV2 cells and primary microglial cells were suspended in culture medium, adjusted to a concentration of 1 9 10 6 -5 9 10 6 cells/mL, plated (2 mL/well) in 6-well cell culture plates, and incubated for 24 h. The cells were randomly assigned to four groups: negative control group, control group, Y27632 (10 lmol/L) group, and fasudil (41 lmol/L) group. The cells in the control, Y27632, and fasudil groups were incubated for 1 h at 37°C with FITCdextran (0.1 mg/mL, molecular weight, 40,000, FD-40s; Sigma) [22,23]. The negative control group was incubated with FITC-dextran at 4°C for 1 h to exclude extracellular binding of FITC-dextran, and was used to set the baseline value in flow cytometry.…”

Section: Fitc-dextran Uptake Analysis By Flow Cytometrymentioning

confidence: 99%

Rho-Associated Kinase Inhibitors Promote Microglial Uptake Via the ERK Signaling Pathway

Tang

et al. 2016

Neurosci. Bull.

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Microglia are immunocompetent cells in the central nervous system that take up tissue debris and pathogens. Rho-associated kinase (ROCK) has been identified as an important regulator of uptake, proliferation, secretion, and differentiation in a number of cell types. Although ROCK plays critical roles in the microglial secretion of inflammatory factors, migration, and morphology, its effects on microglial uptake activity have not been well characterized. In the present study, we found that treatment of BV2 microglia and primary microglia with the ROCK inhibitors Y27632 and fasudil increased uptake activity and was associated with morphological changes. Furthermore, western blots showed that this increase in uptake activity was mediated through the extracellular-signal-regulated kinase (ERK) signaling cascade, indicating the importance of ROCK in regulating microglial uptake activity.

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“…Up to now, clinical studies on this dipeptide have focused on its use as a treatment for respiratory tract infections in children (11,12). Previous studies have demonstrated that pidotimod modulates airway epithelial cell functions (13), enhances natural killer cell activity (14) and induces dendritic cell maturation (15). However, the impact of pidotimod on the regulation of allergic pulmonary inflammation is still not clear.…”

Section: Introductionmentioning

confidence: 99%

Pidotimod exacerbates allergic pulmonary infection in an OVA mouse model of asthma

et al. 2017

Molecular Medicine Reports

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Pidotimod is a synthetic dipeptide with biological and immuno‑modulatory properties. It has been widely used for treatment and prevention of recurrent respiratory infections. However, its impact on the regulation of allergic pulmonary inflammation is still not clear. In the current study, an ovalbumin (OVA)‑induced allergic asthma model was used to investigate the immune‑modulating effects of pidotimod on airway eosinophilia, mucus metaplasia and inflammatory factor expression compared with dexamethasone (positive control). The authors determined that treatment with pidotimod exacerbated pulmonary inflammation as demonstrated by significantly increased eosinophil infiltration, dramatically elevated immunoglobulin E production, and enhanced T helper 2 response. Moreover, treatment failed to attenuate mucus production in lung tissue, and did not reduce OVA‑induced high levels of FIZZ1 and Arg1 expression in asthmatic mice. In contrast, administration of dexamethasone was efficient in alleviating allergic airway inflammation in OVA‑induced asthmatic mice. These data indicated that pidotimod as an immunotherapeutic agent should be used cautiously and the effectiveness for controlling allergic asthma needs further evaluation and research.

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The detailed analysis of the changes of murine dendritic cells (DCs) induced by thymic peptide

Cited by 12 publications

References 22 publications

Immunoactive preparations and regulatory responses in the respiratory tract: potential for clinical application in chronic inflammatory airway diseases

Immunoactive preparations and regulatory responses in the respiratory tract: potential for clinical application in chronic inflammatory airway diseases

Rho-Associated Kinase Inhibitors Promote Microglial Uptake Via the ERK Signaling Pathway

Pidotimod exacerbates allergic pulmonary infection in an OVA mouse model of asthma

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