The Detection and Differentiation of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Endocarditis by Using the BD GeneOhm StaphSR Assay

Frey, Amy; Wilson, Deborah A.; LaSalvia, Margaret M.; Tan, Carmela D.; Rodríguez, E. René; Shrestha, Nabin K.; Hall, Gerri S.; Procop, Gary W.

doi:10.1309/ajcp9l3bhhguaqud

Cited by 4 publications

(1 citation statement)

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“…a Specificity = 100%, sensitivity = 20% our results, because previous studies have documented high sensitivities (100%) in the detection of methicillin-resistant Staphylococcus aureus in formalin-fixed heart valves using the BD GeneOhm PCR assay. 15 Finally, the use of microdissected tissue, as opposed to the entire biopsy specimen, likely did not affect results because C difficile-associated toxins are most concentrated in inflammatory debris and pseudomembranes. Based on these findings, PCR for C difficile in FFPE tissues has very limited use.…”

Section: Discussionmentioning

confidence: 99%

Interobserver Variability and Feasibility of Polymerase Chain Reaction–Based Assay in Distinguishing Ischemic Colitis FromClostridium difficileColitis in Endoscopic Mucosal Biopsies

et al. 2013

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Polymerase chain reaction (PCR)-based assays using stool samples are currently the most effective method of detecting Clostridium difficile. This study examines the feasibility of this assay using mucosal biopsy samples and evaluates the interobserver reproducibility in diagnosing and distinguishing ischemic colitis from C difficile colitis. Thirty-eight biopsy specimens were reviewed and classified by 3 observers into C difficile and ischemic colitis. The findings were correlated with clinical data. PCR was performed on 34 cases using BD GeneOhm C difficile assay. The histologic interobserver agreement was excellent (κ= 0.86) and the agreement between histologic and clinical diagnosis was good (κ = 0.84). All 19 ischemic colitis cases tested negative (100% specificity) and 3 of 15 cases of C difficile colitis tested positive (20% sensitivity). C difficile colitis can be reliably distinguished from ischemic colitis using histologic criteria. The C difficile PCR test on endoscopic biopsy specimens has excellent specificity but limited sensitivity.

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Section: Discussionmentioning

confidence: 99%