The Detection and Nature of Dominant Lethals in Lilium. Ii. Cytological Abnormalities in Ovules After Pollen Irradiation

Cave, Marion S.; Brown, Stephen P. A.

doi:10.1002/j.1537-2197.1954.tb14364.x

Cited by 18 publications

(3 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These data are interesting since most of the data obtained on the consequences of unbalanced gametes on seed development come from studies on irradiated pollen used as pollinator on wild‐type ovules (see e.g. Cave and Brown, 1954; Vizir et al ., 1994). Therefore, spo11‐1 and other synaptic mutants represent new tools to study the impact of unbalanced macro‐ or microspores on gamete development, fertilization and seed development.…”

Section: Discussionmentioning

confidence: 99%

AtSPO11-1 is necessary for efficient meiotic recombination in plants

2001

View full text Add to dashboard Cite

The Saccharomyces cerevisiae Spo11 protein catalyses DNA double-strand breaks (DSBs) that initiate meiotic recombination. The model plant Arabidopsis thaliana possesses at least three SPO11 homologues. T-DNA and ethyl-methane sulfonate mutagenesis allowed us to show that meiotic progression is altered in plants in which the AtSPO11-1 gene is disrupted. Both male and female meiocytes formed very few bivalents. Furthermore, no fully synapsed chromosomes were observed during prophase I. Later, in meiosis I, we observed that chromosomes segregated randomly, leading to the production of a large proportion of non-functional gametes. These meiotic aberrations were associated with a drastic reduction in meiotic recombination. Thus, our data show that initiation of meiotic recombination by SPO11-induced DSBs is a mechanism conserved in plants. Furthermore, unlike Drosophila and Caenorhabditis elegans, but like fungi, SPO11 is necessary for normal synapsis in plants.

show abstract

Section: Discussionmentioning

confidence: 99%

AtSPO11-1 is necessary for efficient meiotic recombination in plants

2001

View full text Add to dashboard Cite

show abstract

“…50% of seeds aborted, while complete seed abortion was observed for a dose of 1200 Gy. Seed abortion can be explained by either dominant lethality, owing to loss of chromosomal DNA causing consequent chromosomal imbalance (Cave & Brown, 1954; Pfahler, 1967) or abnormal development of the endosperm (Lin & Coe, 1986).…”

Section: Discussionmentioning

confidence: 99%

Molecular genetic analysis of pollen irradiation mutagenesis in Arabidopsis

2004

View full text Add to dashboard Cite

Summary• Pollen γ -irradiation mutagenesis was systematically investigated to generate targeted mutations in Arabidopsis .• Irradiation effects on viability, germination and frequency of loci deletions were evaluated.• Mutation frequency increased with irradiation dose and varied depending upon pollen developmental stage. Meiosis was the most irradiation-sensitive stage, however, it did not equate to the highest mutation frequency. High frequencies of targeted mutations were obtained by irradiation from the second mitosis to mature pollen stages, using 400 -600 Gy. Targeted mutations could also be obtained using lower doses of γ -rays (e.g. 200 Gy) provided that pollen was irradiated at an earlier developmental stage. ms1ttg marker locus pseudo-dominants were used to verify the presence and size of the resultant deletions.• The results demonstrate that γ -irradiation of pollen is an efficient approach for generating deletions in the Arabidopsis genome. Pollen mutagenesis offers the possibility of combining single-cell selection procedures with the advantages of haploid systems, including the ability to treat large numbers of pollen grains, the absence of chimerism and direct expression of targeted alleles in the M 1 generation.

show abstract

“…Some authors CAVE, 1953,1954;CAVE and BROWN, 1954;MORGAN and RAPPLEYE, 1951) reported that the percentage of embryoless seeds with endosperm was increased as the dose increased. It was concluded for both maize with its trinucleate pollen and lily with its binucleate one that the polyploid endosperm is influenced less profoundly and accomodates a number of radiation-induced aberrations well in excess of that which is tolerated by the embryo.…”

Section: Dominant Lethalitymentioning

confidence: 99%

Development of Embryo and Endosperm Produced After Irradiation of Pollen in Tradescantia

Vassileva-Dryanovska

2009

Hereditas

View full text Add to dashboard Cite

The Detection and Nature of Dominant Lethals in Lilium. Ii. Cytological Abnormalities in Ovules After Pollen Irradiation

Cited by 18 publications

References 14 publications

AtSPO11-1 is necessary for efficient meiotic recombination in plants

AtSPO11-1 is necessary for efficient meiotic recombination in plants

Molecular genetic analysis of pollen irradiation mutagenesis in Arabidopsis

Development of Embryo and Endosperm Produced After Irradiation of Pollen in Tradescantia

Contact Info

Product

Resources

About