The Detection of Brucella Bacteria with PCR and Bacteriological Method in Raw Milk and Some of the Dairy Products Which Are Consumed in Kars

Gülbaz, Güven; Kamber, Ufuk

doi:10.15835/buasvmcn-vm:11820

Cited by 4 publications

(7 citation statements)

References 27 publications

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“…In addition, several methods for the optimization of DNA extraction from milk to improve subsequent PCR analysis have been set up leading to the reliable detection of Brucella spp. in milk of infected animals (Alamian and Dadar 2019;Ali et al 2014;Gulbaz and Kamber 2016;Kaynak-Onurdag et al 2016;Leal-Klevezas et al 1995). The sensitivity and specificity of the PCR were higher when compared to the culture method, allowing the detection of lower concentrations of Brucella organisms in milk (Amoroso et al 2011;Nimri 2003;Ning et al 2012).…”

Section: Detection Methods and Identification Of Brucella Spp In Thementioning

confidence: 99%

“… 1995 ; Ali et al. 2014 ; Gulbaz and Kamber 2016 ; Kaynak-Onurdag et al. 2016 ; Alamian and Dadar 2019 ).…”

Section: Detection Methods and Identification Of Brucella unclassified

“…It has been reported that the incidence rate of Brucella spp. remains high in different middle income countries, including Iran (Alamian and Dadar 2019;Dadar et al 2019a;Moosazadeh et al 2016), Nigeria (Bale et al 2003;Salisu et al 2017), India (Aulakh et al 2008;Proch et al 2018), Turkey (Gulbaz and Kamber 2016;Kaynak-Onurdag et al 2016), Brazil (Langoni et al 2000;Lemos et al 2018), Pakistan (Ali et al 2013;Ali et al 2014), Egypt (Wareth et al 2017;Wareth et al 2014), China (Ning et al 2013), and Bangladesh (Islam et al 2019). Furthermore, Brucella spp.…”

Section: Brucella Spp Shedding In Milkmentioning

confidence: 99%

“… 2008 ; Proch et al. 2018 ), Turkey (Gulbaz and Kamber 2016 ; Kaynak-Onurdag et al. 2016 ), Brazil (Langoni et al.…”

Section: Brucella Spp Shedding In Milkmentioning

confidence: 99%

See 3 more Smart Citations

Importance of brucellosis control programs of livestock on the improvement of one health

Dadar

Tiwari

Sharun

et al. 2021

Veterinary Quarterly

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Brucellosis not only represents an important health restraint on livestock but also causes high economic losses in many developing countries worldwide. Despite considerable efforts made for the control of brucellosis, the disease is still spreading in many regions (such as the Middle East) where it represents one of the most important health hazards impacting both animals and humans. The present review aims to investigate the efficacy of veterinary control programs regarding brucellosis, with a special focus on current prevention, control, and eradication approaches. The reasons for unsuccessful control programs such as the absence of highly effective vaccines and non-certified bulls are also debated, to understand why the prevalence of brucellosis in livestock is not decreasing in many areas despite considerable efforts taken to date. The importance of governmental and regional investment in brucellosis control remains one of the main limiting factors owing to the limited budget allocated to tackle this disease. In this context, one health concept has generated novel comprehensive approaches with multiple economic implications across the livestock industry and public health. However, the implementation of such global preventive strategies appears to be a key issue for many endemic and low-income countries. According to the collected data, epidemiological contexts including management and trade systems along with well-defined agro-ecological zones should be evaluated in brucellosis endemic countries to improve milk production and to enhance the sustainability of the livestock sector at both national and regional levels.

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Section: Detection Methods and Identification Of Brucella Spp In Thementioning

confidence: 99%

“… 1995 ; Ali et al. 2014 ; Gulbaz and Kamber 2016 ; Kaynak-Onurdag et al. 2016 ; Alamian and Dadar 2019 ).…”

Section: Detection Methods and Identification Of Brucella unclassified

Section: Brucella Spp Shedding In Milkmentioning

confidence: 99%

“… 2008 ; Proch et al. 2018 ), Turkey (Gulbaz and Kamber 2016 ; Kaynak-Onurdag et al. 2016 ), Brazil (Langoni et al.…”

Section: Brucella Spp Shedding In Milkmentioning

confidence: 99%

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Importance of brucellosis control programs of livestock on the improvement of one health

Dadar

Tiwari

Sharun

et al. 2021

Veterinary Quarterly

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show abstract

“…Total of 315 samples of raw milk, cheeses and butter were examined for brucellosis by PCR and bacteriological examination. Pasteurization of milk before consumption is essential to prevent transmission of the pathogen to humans (Gulbaz and Kamber 2016 ). This poses a major public health threat by consuming non-pasteurized milk products produced by unhygienic dairy farms where brucellosis is endemic (Wareth et al 2014 ).…”

Section: Public Health and Economic Importancementioning

confidence: 99%

Bovine brucellosis – a comprehensive review

Khurana

Sehrawat

Tiwari

et al. 2021

Veterinary Quarterly

152

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Brucellosis is a zoonotic disease of great animal welfare and economic implications worldwide known since ancient times. The emergence of brucellosis in new areas as well as transmission of brucellosis from wild and domestic animals is of great significance in terms of new epidemiological dimensions. Brucellosis poses a major public health threat by the consumption of non-pasteurized milk and milk products produced by unhygienic dairy farms in endemic areas. Regular and meticulous surveillance is essentially required to determine the true picture of brucellosis especially in areas with continuous high prevalence. Additionally, international migration of humans, animals and trade of animal products has created a challenge for disease spread and diagnosis in non-endemic areas. Isolation and identification remain the gold standard test, which requires expertise. The advancement in diagnostic strategies coupled with screening of newly introduced animals is warranted to control the disease. Of note, the diagnostic value of miRNAs for appropriate detection of B. abortus infection has been shown. The most widely used vaccine strains to protect against Brucella infection and related abortions in cattle are strain 19 and RB51. Moreover, it is very important to note that no vaccine, which is highly protective, safe and effective is available either for bovines or human beings. Research results encourage the use of bacteriophage lysates in treatment of bovine brucellosis. One Health approach can aid in control of this disease, both in animals and man.

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Presence of Brucella spp. in Milk and Dairy Products: A Comprehensive Review and Its Perspectives

Islam

Rahman

et al. 2023

Journal of Food Quality

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Consuming raw milk and milk-based products that have not been produced under strict control conditions can cause brucellosis, a highly contagious zoonotic disease. It is a significant global public health concern, particularly in regions with poor management and limited resources, such as Latin America, North and East Africa, the Middle East, and South and Central Asia. The study aims to summarize the occurrence of human brucellosis linked to milk and milk products and the presence of Brucella species in dairy foods. To achieve this goal, a meta-analysis was conducted on 69 studies ranging from 2001 to 2022, which were categorized into two groups: the incidence of Brucella species in milk and milk products and the prevalence of human brucellosis resulting from the consumption of contaminated milk. The following milk and milk products showed the highest incidence of Brucella species: cow milk (1.86%–81.7%), buffalo milk (10.4%–61.67%), camel milk (0%–24%), goat milk (0%–88.8%), and cheese (0%–39.1%). Consuming unpasteurized milk and milk products has been identified as the leading cause of human brucellosis, with incidence rates varying from 33.9% to 100%. Several human brucellosis cases have been linked to consuming raw milk and cheese in Spain, Israel, and other countries. Various serological techniques are employed to detect Brucella-specific antibodies in milk. The milk ring test (MRT) and enzyme-linked immunoassay (ELISA) are the two most widely utilized methods for detecting these antibodies in milk. Recently developed dual biosensors are a powerful approach for early diagnosis of Brucella from milk. Real-time PCR can rapidly detect organisms, reducing the risk of lab contamination and false positive results. To prevent and control brucellosis, essential steps include proper pasteurization of milk and dairy products, using the milk ring test (MRT) to detect Brucella in individual and bulk milk, immunization, education, and increasing public awareness of the disease. The consumption of raw milk and milk-made products that are not produced under strictly controlled conditions poses a significant risk to human health, mainly due to the high incidence of Brucella contamination. Therefore, ensuring strict control measures in producing milk and milk-made products is crucial to preventing the spread of this disease and safeguarding human health.

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The Detection of Brucella Bacteria with PCR and Bacteriological Method in Raw Milk and Some of the Dairy Products Which Are Consumed in Kars

Cited by 4 publications

References 27 publications

Importance of brucellosis control programs of livestock on the improvement of one health

Importance of brucellosis control programs of livestock on the improvement of one health

Bovine brucellosis – a comprehensive review

Presence of Brucella spp. in Milk and Dairy Products: A Comprehensive Review and Its Perspectives

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