The detection of hydroxyl radicals in vivo

Freinbichler, Wolfhardt; Bianchi, Luigi; Colivicchi, Maria Alessandra; Ballini, Chiara; Tipton, Keith F.; Linert, Wolfgang; Corte, Laura Della

doi:10.1016/j.jinorgbio.2007.12.017

Cited by 45 publications

(27 citation statements)

References 49 publications

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“…Our own results in artificial cerebrospinal fluid (aCSF) [28, 43,44] have confirmed and extended this by showing that the reactive intermediate is not the free hydroxyl radical, but either a crypto radical or a ferryl species; with the evidence supporting the description of the reactive species as a crypto radical.…”

Section: Introductionmentioning

confidence: 64%

“…Further advances in our understanding necessitate the application of sensitive and reliable methods for hROS detection in vivo, such as those discussed in this review. Their use in microdialysis experiments to detect hROS in vivo, in freely moving animals, has considerable potential for research and medicine [see 43,44]. However, caution and rigor are essential for the correct interpretation of the data obtained.…”

Section: Discussionmentioning

confidence: 99%

“…3. Although widely used, this method has several fundamental disadvantages, which have been described in detail elsewhere [see 28, 43,44]. Essentially the drawbacks of this method are the hydroxylation behavior and bioactivity of SA.…”

Section: Salicylate (Sa)mentioning

confidence: 99%

“…We showed in our previous work that TA does not influence the basal and the evoked levels of the neurotransmitters, glutamate, aspartate, taurine, and GABA (c-aminobutyrate), in microdialysis experiments using kainate stimulation. Since a significant linear correlation between the formation of OH-TA and kainate concentration was found, it was concluded that it can be used for reliable hROS quantification [43,44]. TA does not interfere with the use of o-phthalaldehyde (OPA) for derivatizing amino acids, and therefore can be used for the determination of the release of these transmitters and hROS formation in the same microdialysis experiment [43].…”

Section: -Hydroxybenzoic Acid (4-hba)mentioning

confidence: 99%

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Highly reactive oxygen species: detection, formation, and possible functions

Freinbichler

Colivicchi

Stefanini

et al. 2011

Cell. Mol. Life Sci.

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141

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The so-called reactive oxygen species (ROS) are defined as oxygen-containing species that are more reactive than O(2) itself, which include hydrogen peroxide and superoxide. Although these are quite stable, they may be converted in the presence of transition metal ions, such as Fe(II), to the highly reactive oxygen species (hROS). hROS may exist as free hydroxyl radicals (HO·), as bound ("crypto") radicals or as Fe(IV)-oxo (ferryl) species and the somewhat less reactive, non-radical species, singlet oxygen. This review outlines the processes by which hROS may be formed, their damaging potential, and the evidence that they might have signaling functions. Since our understanding of the formation and actions of hROS depends on reliable procedures for their detection, particular attention is given to procedures for hROS detection and quantitation and their applicability to in vivo studies.

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Section: Introductionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Section: Salicylate (Sa)mentioning

confidence: 99%

Section: -Hydroxybenzoic Acid (4-hba)mentioning

confidence: 99%

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Highly reactive oxygen species: detection, formation, and possible functions

Freinbichler

Colivicchi

Stefanini

et al. 2011

Cell. Mol. Life Sci.

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141

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“…A number of methods have been reported for detection of hydroxyl radicals in cellular [50] and cellfree systems. [51,52] In cell-free systems, hydroxylation of deoxyribose [53][54][55] can be considered to be the most relevant method, as it models the process of DNA strain breaks. Degradation of www.chembiochem.org deoxyribose caused by in situ-generated ROS [53][54][55] was determined by the standard assay using condensation of the resultant malonic dialdehyde with thiobarbituric acid (Figure 5 A), which produced UV-Vis absorption with l max = 532 nm.…”

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confidence: 99%

Biomolecular Oxidative Damage Activated by Enzymatic Logic Systems: Biologically Inspired Approach

et al. 2009

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Systems that perform oxidative damage to biomolecules through catalytic cascades in the presence of iron-redox labile species were activated by enzymatic logic gates that process chemical input signals according to built-in logic operations. AND/OR enzymatic logic gates were composed of glucose oxidase (GOx) and GOx/esterase, respectively. The AND/OR logic functions of the enzyme gates were activated by application of glucose-oxygen and glucose-ethyl acetate input signals, respectively. The enzymatic logic gates, upon activation by specific patterns of the chemical input signals, produced acidic solutions and triggered release of redox labile iron species from a complex that is unstable under acidic conditions. This resulted in the activation of a catalytic cascade, which produced reactive oxygen species (ROS) and subsequently yielded oxidative damage in biomolecules. Functional integration of the enzyme-based logic systems with the catalytic redox cascade that performs damage in biomolecules on demand is a first step towards "smart" systems capable of programmed detection, identification, and neutralization of potential biohazards.

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Chemistry of Reactive Species

Villamena

2013

Molecular Basis of Oxidative Stress

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The detection of hydroxyl radicals in vivo

Cited by 45 publications

References 49 publications

Highly reactive oxygen species: detection, formation, and possible functions

Highly reactive oxygen species: detection, formation, and possible functions

Biomolecular Oxidative Damage Activated by Enzymatic Logic Systems: Biologically Inspired Approach

Chemistry of Reactive Species

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