The detection of occult HBV infection in patients with HBsAg negative pattern by real-time PCR method

Izmirli, Sena; Çelik, Deniz; Yüksel, Pelin; Sarıbaş, Suat; Aslan, Mustafa; Erǧin, Sevgi; Bahar, Hrisi; Sen, Sümeyye; Çakal, Bülent; Öner, Ali Yusuf; Kocazeybek, Bekir

doi:10.1016/j.transci.2012.07.009

Cited by 12 publications

(8 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…HBV infection is identified in most cases by the presence of circulating hepatitis B surface antigen (HBsAg), but an HBsAg-negative HBV infection has also been described [Occult B infection (OBI)] [7] , characterized by low levels of HBV DNA in circulating blood [8,9] and/or in liver tissue [10] . OBI has also been described as a serological condition characterized by the presence of hepatitis B core antigen (anti-HBc) in the absence of HBsAg and anti-HBs (isolated anti-HBc) [7,[11][12][13][14][15] .…”

Section: Introductionmentioning

confidence: 99%

Clinical impact of occult hepatitis B virus infection in immunosuppressed patients

Sagnelli

Pisaturo

Martini

et al. 2014

WJH

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Clinical impact of occult hepatitis B virus infection in immunosuppressed patients

Sagnelli

Pisaturo

Martini

et al. 2014

WJH

View full text Add to dashboard Cite

“…Although in the majority of these cases this profile is due to past HBV infection there is a possibility of occult chronic HBV infection. Occult HBV infection is defined as HBsAg negative and HBV DNA positive in serum and/or liver [ 68 , 69 , 70 , 71 , 72 , 73 ]. In circumstances where patients positive for anti-HBc only, are going to receive immunosuppressive therapy it is imperative to be tested for HBV viremia with NAT (HBV DNA).…”

Section: Clinical Significance Of Viral Biomarkers In Hbeag(-) Infmentioning

confidence: 99%

Viral Biomarkers in Chronic HBeAg Negative HBV Infection

Hadziyannis

Laras

2018

Genes

View full text Add to dashboard Cite

Viral biomarkers are important tools for monitoring chronic hepatitis B virus (HBV) hepatitis B early antigen (HBeAg) negative infection, both in its natural course as well as during and after treatment. The biomarkers consist of antibodies against viral epitopes, viral proteins, and molecular surrogate markers of the quantity and transcriptional activity of the stable episomal HBV covalently closed circular DNA (cccDNA) which is located in the nuclei of the infected hepatocytes. HBV deoxyribonucleic acid (DNA) or else viral load measurement in plasma or serum is a marker of HBV replication of major clinical importance. HBV DNA is used for staging and treatment monitoring as described in international scientific guidelines. Quantification of HBV antigens, mainly hepatitis B surface antigen (HBsAg) as well as Hepatitis B core related antigen (HBcrAg), play an important yet secondary role, especially in cases of low or undetectable HBV DNA and has been evaluated for the classification of the inactive carrier state, as a predictor of subsequent HBsAg clearance, treatment outcome, and development of hepatocellular carcinoma (HCC). The measurement of the replicative intermediate HBV RNA in serum is currently evaluated and may also prove to be a significant biomarker particularly in patients treated with nucleot(s)ide analogs. This review focuses on the viral biomarkers mentioned above and their role in HBV, HBeAg negative, infection.

show abstract

“…The system is versatile, can be used for many other approaches and it does not require further manipulations in order to reduce the risk of contamination. Real time PCR is certainly the future of diagnostics, and improved standardization will make it useful in the management of patients with HBV infection [41].…”

Section: Real Time Pcrmentioning

confidence: 99%

Advance in molecular diagnostic tools for hepatitis B virus detection

Pazienza

Niro

Fontana

et al. 2013

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

The rapid evolution of molecular biology techniques has a significant impact on laboratory medicine. Nowadays a large number of diagnostic tools are available to diagnose and to characterize the different phases of hepatitis B virus (HBV) infection. The advent of the assay for nucleic acid amplification and detection enables clinicians to initiate and monitor antiviral therapy whilst allowing basic scientists to carry out studies on HBV biology. This review will focus on the evolution of the diagnostic tools to detect and monitor HBV infection.

show abstract

The detection of occult HBV infection in patients with HBsAg negative pattern by real-time PCR method

Cited by 12 publications

References 23 publications

Clinical impact of occult hepatitis B virus infection in immunosuppressed patients

Clinical impact of occult hepatitis B virus infection in immunosuppressed patients

Viral Biomarkers in Chronic HBeAg Negative HBV Infection

Advance in molecular diagnostic tools for hepatitis B virus detection

Contact Info

Product

Resources

About