The detection of varicella zoster antibodies by simultaneous competitive EIA and its comparison with radioimmunoassay, latex agglutination and antiglobulin type EIA

Unadkat, P.; Newman, Barbara A.; Tedder, Richard S.

doi:10.1016/0166-0934(94)00096-y

Cited by 6 publications

(6 citation statements)

References 6 publications

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“…However, in the study, VZVScan seemed to lack specificity because of false-positive interpretations of the assay (9). Two other studies also have shown good correlation between EIA and VZVScan (8,11). Both of these studies, however, had minor problems with false-negative reactions and prozone effects with VZVScan.…”

mentioning

confidence: 72%

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Determination of varicella-zoster virus (VZV) immune status with the VIDAS VZV immunoglobulin G automated immunoassay and the VZVScan latex agglutination assay

Gleaves

Schwarz

Campbell

1996

Clin Diagn Lab Immunol

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The VIDAS varicella-zoster virus (VZV) immunoglobulin G immunoassay and the VZVScan latex agglutination (LA) test were compared for the detection of VZV antibody in patient sera. Of 625 samples tested, 554 were positive for VZV antibody by one or both methods. Five hundred thirteen (82.1%) samples were positive by both methods, 71 (11.4%) were negative by both methods, and there were 41 (6.6%) discrepant samples. Statistically significant differences in sensitivity and specificity were not found (P ‫؍‬ 0.37); however, several observations were made. All 23 VIDAS repeat equivocal samples were LA positive. Fifty-four samples showed prozone effects with the LA assay, all of which resolved as positive.

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confidence: 72%

“…Several methods are available for detection of VZV antibody from patient sera (1,7,10,11,13). However, rapid, sensitive, and easy-to-perform assays are needed clinically in determining the immune status of individuals with no prior history or memory of varicella infection (2).…”

mentioning

confidence: 99%

Determination of varicella-zoster virus (VZV) immune status with the VIDAS VZV immunoglobulin G automated immunoassay and the VZVScan latex agglutination assay

Gleaves

Schwarz

Campbell

1996

Clin Diagn Lab Immunol

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“…Samples were frozen and stored at -30°C ± 10°C. Serum samples were analyzed for quantitation of VZV-specific antibodies by a semiautomated, amplified antibody capture enzyme-linked immunosorbent assay 7 and Standard Test Method Validation No. 53, STM 500605.00 (Cangene, Winnipeg, Manitoba).…”

Section: Vzig Concentration Measurementsmentioning

confidence: 99%

Serum Concentrations, Efficacy, and Safety of a New, Intravenously Administered Varicella Zoster Immune Globulin in Pregnant Women

Koren

Money

Boucher

et al. 2002

The Journal of Clinical Pharma

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Chickenpox is teratogenic in humans, and varicella zoster immune globulin (VZIG) is given to pregnant women believed to be susceptible to the virus after contact with chickenpox. Available VZIG is given as intramuscular injections. The objective of this study was to evaluate the efficacy, safety, and serum concentrations of a new VZIG that can be given intravenously. The new VZIG (Cangene Pharm., Inc.) was compared to the standard VZIG (Massachusetts Public Health Biologic Laboratories) in a randomized protocol in 57 pregnant women seronegative to varicella zoster virus (VZV). Pregnant women received 125 units per 10 kg body weight to a maximal dose of 625 units. Women were evaluated on days 2, 7, 14, and 28 and at other times if symptoms developed into clinical varicella, which was scored by the Constitutional Illness Score. The new VZIG was comparable to the standard VZIG on all parameters of efficacy and safety. Levels of VZV antibodies at day 2 postinjection were significantly higher among those receiving the new preparation intravenously. The authors concluded that the new intravenous form of VZIG confers higher initial levels of VZV antibodies and is comparable in terms of its maternal efficacy and safety to the standard form of VZIG.

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“…Not unexpectedly, there was a significantly high correlation between VZV seropositivity and a positive history of chickenpox, reflecting the reliability of the latex agglutination test which has been compared with other standard methods, eg, fluorescent antibody assay, enzyme immunoassay and radioimmunoassay [2,3]. Conversely, 30 students (10.2%) tested negative for VZV antibodies despite a previous history of symptoms of chickenpox.…”

Section: Discussionmentioning

confidence: 96%

Rapid detection of varicella-zoster viral antibodies by latex agglutination assay: a practical experience for medical and science undergraduates

Chow

1998

Biochemical Education

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The detection of varicella zoster antibodies by simultaneous competitive EIA and its comparison with radioimmunoassay, latex agglutination and antiglobulin type EIA

Cited by 6 publications

References 6 publications

Determination of varicella-zoster virus (VZV) immune status with the VIDAS VZV immunoglobulin G automated immunoassay and the VZVScan latex agglutination assay

Determination of varicella-zoster virus (VZV) immune status with the VIDAS VZV immunoglobulin G automated immunoassay and the VZVScan latex agglutination assay

Serum Concentrations, Efficacy, and Safety of a New, Intravenously Administered Varicella Zoster Immune Globulin in Pregnant Women

Rapid detection of varicella-zoster viral antibodies by latex agglutination assay: a practical experience for medical and science undergraduates

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