Cassava is a very important crop in Nigeria by reason of its comparative production advantage over other staples.This study estimated the relative profitability of cassava producers and determined the effects of farm inputs on the level of profit of various scales of cassava producers in Ogun and Oyo States, Nigeria. Cross-sectional data were collected from 265 cassavabased farmers using a multistage sampling technique and were analysed using normalized profit function and budgetary analysis. Results showed that cassava/cowpea enterprise had the highest net margins of N127,249.63/ha and N122,325.73/ha in Ogun and Oyo States respectively. While the use of herbicides had a positive and significant effect on the profitability of small-scale cassava farmers in both states, cassava-cuttings had a positive and significant effect on medium-scale cassava farming in both states. Similarly, labour and farm size had a positive and significant effect on large-scale cassava farming in both states. The study recommends that small and medium-scale farmers should increase the application of herbicide, large-scale cassava operators in Oyo State should increase the use of labour, while those in Ogun State should also increase the cultivated acreage of land.