The Determination, Monitoring, Molecular Mechanisms and Formation of Biofilm in E. coli

Öztürk, Fırat Yavuz; Darcan, Cihan; Kariptaş, Ergin

doi:10.1007/s42770-022-00895-y

Cited by 17 publications

(8 citation statements)

References 158 publications

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“…Then, the bacteria form microcolonies when their adhesion to the biomaterial surface is stable. Finally, when the external conditions change, such as the temperature, nutrition, pH, or oxygen, the biofilm will disperse and the bacteria will return to the planktonic state [17,18].…”

Section: Biofilm Formation On Biomaterialsmentioning

confidence: 99%

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Bacterial Biofilm Formation on Biomaterials and Approaches to Its Treatment and Prevention

Yin

Cheng

et al. 2023

IJMS

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Bacterial biofilms can cause widespread infection. In addition to causing urinary tract infections and pulmonary infections in patients with cystic fibrosis, biofilms can help microorganisms adhere to the surfaces of various medical devices, causing biofilm-associated infections on the surfaces of biomaterials such as venous ducts, joint prostheses, mechanical heart valves, and catheters. Biofilms provide a protective barrier for bacteria and provide resistance to antimicrobial agents, which increases the morbidity and mortality of patients. This review summarizes biofilm formation processes and resistance mechanisms, as well as the main features of clinically persistent infections caused by biofilms. Considering the various infections caused by clinical medical devices, we introduce two main methods to prevent and treat biomaterial-related biofilm infection: antibacterial coatings and the surface modification of biomaterials. Antibacterial coatings depend on the covalent immobilization of antimicrobial agents on the coating surface and drug release to prevent and combat infection, while the surface modification of biomaterials affects the adhesion behavior of cells on the surfaces of implants and the subsequent biofilm formation process by altering the physical and chemical properties of the implant material surface. The advantages of each strategy in terms of their antibacterial effect, biocompatibility, limitations, and application prospects are analyzed, providing ideas and research directions for the development of novel biofilm infection strategies related to therapeutic materials.

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Section: Biofilm Formation On Biomaterialsmentioning

confidence: 99%

“…After the formation of the first biofilm structure mediated by EPS, other bacteria of the same species and other species in the environment are also incorporated into the biofilm structure. The biofilm structure then develops from a thin layer into a mushroom-like or other three-dimensional structure [17,[44][45][46].…”

Section: Biofilm Maturationmentioning

confidence: 99%

Bacterial Biofilm Formation on Biomaterials and Approaches to Its Treatment and Prevention

Yin

Cheng

et al. 2023

IJMS

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“…The bacteria/surface interaction is complicated by specific bacterial responses that are pertinent for biofilm formation including communal response via quorum sensing . More work needs to explore the bacterial responses to a variety of surfaces.…”

Section: Discussionmentioning

confidence: 99%

“…Escherichia coli (E. coli) is a model Gram-negative bacterium that has played significant roles in studies of adhesion and biofilm formation E.…”

Section: Introductionmentioning

confidence: 99%

Escherichia coli Adhesion and Biofilm Formation on Polymeric Nanostructured Surfaces

Iyer,

Laws,

LaJeunesse

2023

ACS Omega

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Biofilm formation is a multistep process that requires initial contact between a bacterial cell and a surface substrate. Recent work has shown that nanoscale topologies impact bacterial cell viability; however, less is understood about how nanoscale surface properties impact other aspects of bacterial behavior. In this study, we examine the adhesive, viability, morphology, and colonization behavior of the bacterium Escherichia coli on 21 plasma-etched polymeric surfaces. Although we predicted that specific nanoscale surface structures of the surface would control specific aspects of bacterial behavior, we observed no correlation between any bacterial response or surface structures/properties. Instead, it appears that the surface composition of the polymer plays the most significant role in controlling and determining a bacterial response to a substrate, although changes to a polymeric surface via plasma etching alter initial bacteria colonization and morphology.

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“…1 ml of this dissolved dye was read in the spectrophotometer at OD 540 nm, and biofilm formation (strong–weak) levels were detected. Biofilm formation in the tube and absorbance measurement were performed after staining with crystal violet [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of presence of clone ST131 and biofilm formation in ESBL producing and non-producing Escherichia coli strains

Çelebi

Aydın²,

Rakici

et al. 2023

Mol Biol Rep

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Objective Escherichia coli ST131 is a pandemic clone associated with multidrug resistance, starting with beta-lactamase production and fluoroquinolone resistance in the first place, leading to significant systemic infections. Clones that develop due to the frequency of antimicrobial resistance and the rate of spread in our country are important issues that need to be investigated. This study aims to investigate the incidence of ST131which is a “high-risk pandemic clone E. coli ” in ESBL-producing and non-ESBL-producing strains, as well as their biofilm-forming abilities and antibiotic resistance rates. Materials and methods A total of 86 E. coli isolates were used in the study. Bacterial identifications were performed by conventional and automated methods. The double disc synergy method was used to demonstrate the presence of ESBL. Molecular studies in all E. coli strains were performed by real-time PCR method. Findings: 86 strains were studied, of which 83.72% were urine, 6.98% were wound, 4.65% were blood, and 2.33% were tracheal aspirate and sputum. 79.07% of these strains were ESBL-positive. 58.1% of the strains were female, whereas 41.9% were male patients, and the average age was 46.2. Out of 86 strains, 38.72% were ST131 positive, the H30 subclone was detected in 27.27% of them, and the H30-Rx subclone was detected in all of the H30 subclone positive strains. The presence of the ESBL resistance gene was detected at the rate of TEM 41.86%, SHV 37.21%, CTX-M 36.04%, and OXA 4.65%. Most commonly SHV gene (54.54%) was seen in ST131 clone-positive samples. Finally, while it was found that 48.83% of the strains formed biofilm by any method, biofilm formation was detected in 69.7% of the samples that were positive for the ST131 clone. Result Our study can reveal the dramatic prevalence of the ESBL-producing E. coli strains along with the high-risk ST131 clone, the dominance of the H30Rx subclone of this risky clone, as well as the importance of the influence of resistance mechanisms along with resistance and biofilm.

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The Determination, Monitoring, Molecular Mechanisms and Formation of Biofilm in E. coli

Cited by 17 publications

References 158 publications

Bacterial Biofilm Formation on Biomaterials and Approaches to Its Treatment and Prevention

Bacterial Biofilm Formation on Biomaterials and Approaches to Its Treatment and Prevention

Escherichia coli Adhesion and Biofilm Formation on Polymeric Nanostructured Surfaces

Evaluation of presence of clone ST131 and biofilm formation in ESBL producing and non-producing Escherichia coli strains

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