The Determination of Ascorbic Acid in Whole Blood and Urine Through the 2,4-Dinitrophenylhydrazine Derivative of Dehydroascorbic Acid

Roe, Joseph H.; Kuether, Carl A.

doi:10.1016/s0021-9258(18)72395-8

Cited by 1,484 publications

(71 citation statements)

References 5 publications

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“…The absorbance of clear supernatant was measured against the reference blank at 535 nm. • Vitamin C: This was estimated by Roe and Kuether's method [22]. About 0.5 mL of the serum sample was mixed with 1.5 mL of 6% TCA and then centrifuged.…”

Section: Laboratory Analysesmentioning

confidence: 99%

Evaluation of oxidative markers in women with invasive cervical cancer in Lagos, Nigeria

Offor¹,

Okunade²,

Iwalokun³

et al. 2021

ecancer

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Epidemiological studies have showed that low levels of antioxidants induce the generation of free radicals leading to DNA damage and further mutations seen in cancer. This study evaluated the effects of oxidative markers on the occurrence and severity of cervical cancer at the Lagos University Teaching Hospital. This was an analytical crosssectional study carried out among women with histological diagnosis of invasive cervical cancer and their healthy cancer-free comparison group. Venous blood samples were collected from each participant for measurements of antioxidants (erythrocyte glutathione and vitamin C) and malondialdehyde (a marker of lipid peroxidation). Descriptive statistics were carried out for relevant demographic and clinical data. Associations between continuous variables were tested using the independent sample t-test or the analysis of variance for normally distributed data or the Mann-Whitney U test for skewed data, whereas categorical variables were compared using the χ 2 test. p < 0.05 was considered statistically significant. The mean level of malondialdehyde (MDA) was statistically higher in women with cervical cancer than in their cancer-free counterparts (p = 0.032). However, the mean glutathione (32.6 ± 6.2 versus 14.2 ± 6.1 mg/dL; p = 0.019) and vitamin C (12.4 ± 2.3 versus 14.6 ± 2.4 µmol/L; p = 0.001) levels were significantly lower in the case group compared to the cancer-free group. There are statistically increasing mean levels of MDA (p = 0.017) and decreasing mean levels of vitamin C (p = 0.004) with increasing stages of the disease. This study showed that women with cervical cancer have low levels of antioxidants and an increased level of the oxidative marker. The levels of these markers become more pronounced as the disease progresses. This will, therefore, form the basis for the conduct of future randomised controlled trials of antioxidant supplementations among cervical cancer patients in sub-Saharan Africa.

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Section: Laboratory Analysesmentioning

confidence: 99%

Evaluation of oxidative markers in women with invasive cervical cancer in Lagos, Nigeria

Offor¹,

Okunade²,

Iwalokun³

et al. 2021

ecancer

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“…The determination of ascorbic acid activity was done according to the protocol given by Roe and Kuether. 20…”

Section: Antioxidant Parametersmentioning

confidence: 99%

Chemomodulatory Potential of Flaxseed Oil Against DMBA/Croton Oil–Induced Skin Carcinogenesis in Mice

2016

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The present study was conducted to evaluate the potential of flaxseed oil to prevent chemically induced skin cancer in mice. Cancer was induced on 2-stage skin carcinogenesis model by single topical application of 7,12 dimethylbenz [a]anthracene (DMBA), as, initiator, and two weeks later it was promoted by croton oil treatment thrice a week on the dorsal surface of mice for 16 weeks. Flaxseed oil (FSO; 100µL/animal/d) was orally administered 1 week before and 1 week after DMBA application (Peri-initiation stage). The animals of the FSO-administered group showed a significant reduction in tumor incidence (76.67%), cumulative number of tumors (37), tumor yield (3.7), and tumor burden (4.81) when compared with the carcinogen-treated control animals. Biochemical parameters in skin and liver tissue such as LPO and phase I enzymes were significantly (P < .01) reduced in the FSO-treated experimental group, whereas the phase II enzymes (GST, DT-diaphorase) and antioxidant parameters (GSH, GPx, SOD, catalase, and vitamin C) exhibited a significant (P < .01) elevation when compared with the animals of the carcinogen-treated control group. Histopathological alterations in the carcinogen-treated control animals were also observed in the form of epidermal hyperplasia, keratinized pearl formation, and acanthosis in skin and tumors, whereas these were found to be reduced after FSO administration. The results of the present study demonstrate that the oral administration of FSO has the potential to modulate the levels of LPO, antioxidants, and detoxification enzymes in the DMBA-croton oil–induced skin carcinogenesis in mice.

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“…Supernatant was used for the assay of LPO, 20 superoxide dismutase (SOD), 21 catalase (CAT), 22 reduced glutathione (GSH) 23 and total protein content estimation, 24 while ascorbic acid (AA) content was determined in sediment by the method of Roe and Küether. 25 c) Microscopic examination of liver Liver samples were fixed in 4% buffered paraformaldehyde, dehydrated in graded alcohol series and embedded in paraffin wax. About 5-mm thick sections were cut (by Leica RM 2155 Microtome) and stained with hematoxyline and eosin and examined under Leica microscope.…”

Section: B) Hepatic Lipid Peroxidation and Antioxidantsmentioning

confidence: 99%

Toxicological evaluation and hepatoprotective potential of Clerodendron glandulosum.Coleb leaf extract

et al. 2010

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This inventory evaluates toxicological effects and hepatoprotective potential of Clerodendron glandulosum.Coleb (CG) aqueous extract. Acute and subchronic toxicity tests were performed using Swiss albino mice as per the guideline of Organisation for Economic Cooperation and Development (OECD). Also, hepatoprotective potential of CG extract was examined in experimental model of carbon tetrachloride (CCl 4)-induced hepatotoxicity. Acute and subchronic toxicity tests revealed that CG extract is non-toxic and its median lethal dose (LD50) value is >5000 mg/kg bodyweight. Also, rats pretreated with CG extract followed by administration of CCl4 recorded significant decrement in plasma marker enzymes of hepatic damage, total bilirubin content and hepatic lipid peroxidation. While, hepatic reduced glutathione, ascorbic acid content, activity levels of superoxide and catalase and plasma total protein content were significantly increased. Microscopic examination of liver showed that pretreatment with CG extract prevented CCl4-induced hepatic damage in CG + CCl 4 group. CG extract has hepatoprotective potential by modulating activity levels of enzymes and metabolites governing liver function and by helping in maintaining cellular integrity of hepatocytes that is comparable with that of standard drug silymarin. CG extract exhibits potent hepatoprotective activity against CCl4-induced hepatic damage but does not exhibit any toxic manifestations.

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The Determination of Ascorbic Acid in Whole Blood and Urine Through the 2,4-Dinitrophenylhydrazine Derivative of Dehydroascorbic Acid

Cited by 1,484 publications

References 5 publications

Evaluation of oxidative markers in women with invasive cervical cancer in Lagos, Nigeria

Evaluation of oxidative markers in women with invasive cervical cancer in Lagos, Nigeria

Chemomodulatory Potential of Flaxseed Oil Against DMBA/Croton Oil–Induced Skin Carcinogenesis in Mice

Toxicological evaluation and hepatoprotective potential of Clerodendron glandulosum.Coleb leaf extract

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