The Determination of Equilibrium Constants from Transport Data on Rapidly Reacting Systems of the Type A + B ⇌ C

Nichol, L.W.; Winzor, Donald J.

doi:10.1021/j100791a012

Cited by 119 publications

(47 citation statements)

References 8 publications

(12 reference statements)

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“…A detailed description of the FA concept is provided in Section 2.2 below. FA was first applied in a gel filtration setup by Nichol and Winzor [18] in 1964 studying protein-protein interactions. The methodology was extended to the study of small molecule (drug)-protein interactions by Scholtan [19] and by Cooper and Wood [20].…”

Section: Frontal Analysis Methodologiesmentioning

confidence: 99%

“…The elution profiles thus generated allow the determination of free ligand concentration from the height of the free drug plateau [20,21,26,27]. Although CE-FA appears to have evolved from FA chromatography [3], it should be recognized that the theory on which FA chromatography is based was originally developed for electrophoresis and ultracentrifugation [18,31]. Thus, there is a link from CE-FA back to early affinity studies performed using moving boundary electrophoresis, see [32] for a historical presentation.…”

Section: Frontal Analysis Methodologiesmentioning

confidence: 99%

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Capillary electrophoresis frontal analysis: Principles and applications for the study of drug‐plasma protein binding

2003

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Capillary electrophoresis frontal analysis: Principles and applications for the study of drug-plasma protein bindingCapillary electrophoresis is a well-established technique for the study of noncovalent interactions. Various approaches exist and capillary electrophoresis-frontal analysis provides an interesting alternative to the migration shift affinity capillary electrophoresis methods and conventional methods. The present work reviews the principles on which the frontal analysis method is founded. Advantages and limitations of capillary electrophoresis frontal analysis in comparison with both conventional and other capillary electrophoresis based methods for quantification of binding interactions are discussed. Investigations utilizing capillary electrophoresis-frontal analysis have focused on the interaction of drugs with plasma proteins. These studies, primarily addressing the binding of drugs to human serum albumin, a 1 -acid glycoprotein, and lipoproteins are reviewed together with some recent developments in capillary electrophoresisfrontal analysis methodology.

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Section: Frontal Analysis Methodologiesmentioning

confidence: 99%

Section: Frontal Analysis Methodologiesmentioning

confidence: 99%

Capillary electrophoresis frontal analysis: Principles and applications for the study of drug‐plasma protein binding

2003

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“…An obvious outcome of these deliberations is the conclusion that nonspecific binding is more amenable to characterization by methods such as frontal size exclusion chromatography (Nichol and Winzor 1964;Hogg and Winzor 1985;Cann and Winzor 1987) under conditions where the extent of ligand binding can be measured directly as the difference between free and total ligand concentrations. Ironically, virtually all attempts to characterize the thrombin-heparin interaction have entailed spectral procedures for indirect determination of the extent of complex formation from the ratio of equilibrium response to the maximal response (as in Fig.…”

Section: Discussionmentioning

confidence: 99%

A historical perspective of the biophysics of the thrombin–heparin system: an example of nonspecific binding and the consequent parking problem in action

Winzor

2013

Biophys Rev

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Difficulties are encountered in the thermodynamic characterization of interactions between a protein ligand and a linear acceptor, such as a polynucleotide or a polysaccharide, because of the involvement of more than one unit of the polymer chain in each attachment of a protein molecule. Complications arise from the fact that random attachment of ligand to the polymer chain, each unit of which is a potential binding site, initially leads to suboptimal location of protein molecules along the polymer chain-a situation that has to be rectified before the attainment of thermodynamic equilibrium can be realized. Kinetic as well as thermodynamic consequences of such nonspecific binding, termed the parking problem, therefore need to be considered in any quantitative characterization of the interaction between a large ligand and a linear polymer acceptor chain. Results for the thrombin-heparin interaction have been used to illustrate a thermodynamic characterization of nonspecific binding that takes into account these consequences of the parking problem.

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“…Upon his return to Sydney, Michael Tracey, Leader of the Wheat Research Unit, encouraged Don to continue basic research into the characterisation of interacting systems. Those academic pursuits, performed in conjunction with Laurie Nichol, continued to the end of 1967 (Nichol and Winzor 1964;Winzor and Nichol 1965;Nichol et al 1967a, b, c), at which stage Don moved to Brisbane to take up an appointment as Senior Lecturer in the Department of Biochemistry at the University of Queensland.…”

Section: Early Life and Universitymentioning

confidence: 99%

Foreword to ‘Quantitative and analytical relations in biochemistry’—a special issue in honour of Donald J. Winzor’s 80th birthday

Hall

Harding

2016

Biophys Rev

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The purpose of this special issue is to honour Professor Donald J. Winzor's long career as a researcher and scientific mentor, and to celebrate the milestone of his 80th birthday. Throughout his career, Don has been renowned for his development of clever approximations to difficult quantitative relations governing a range of biophysical measurements. The theme of this special issue, 'Quantitative and analytical relations in biochemistry', was chosen to reflect this aspect of Don's scientific approach.

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The Determination of Equilibrium Constants from Transport Data on Rapidly Reacting Systems of the Type A + B ⇌ C

Cited by 119 publications

References 8 publications

Capillary electrophoresis frontal analysis: Principles and applications for the study of drug‐plasma protein binding

Capillary electrophoresis frontal analysis: Principles and applications for the study of drug‐plasma protein binding

A historical perspective of the biophysics of the thrombin–heparin system: an example of nonspecific binding and the consequent parking problem in action

Foreword to ‘Quantitative and analytical relations in biochemistry’—a special issue in honour of Donald J. Winzor’s 80th birthday

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