The Determination of Glycogen in Liver and Muscle by Use of Anthrone Reagent

Carroll, Nicholas V.; Longley, Robert W.; Roe, Joseph H.

doi:10.1016/s0021-9258(18)65284-6

Cited by 1,109 publications

(89 citation statements)

References 9 publications

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“…The ash content was determined by combusting the sample in a muffle furnace at 550°C for 6 h (AOAC 1997). The glycogen content was determined using the method reported by Carroll et al (1956).…”

Section: Proximate Analysismentioning

confidence: 99%

Evaluation of protein: lipid ratio on growth, feed efficiency, and metabolic response in juvenile yellowtail snapper Ocyurus chrysurus (Bloch, 1791)

Arenas

Álvarez-González²,

Barreto

et al. 2021

Lat. Am. J. Aquat. Res.

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This study was conducted to evaluate juveniles' Ocyurus chrysurus (13.7 ± 0.45 g initial weight) to utilize lipid as an energy source on growth, feed efficiency, body composition, digestive and hepatic enzyme activities. Four diets of two protein levels (40 and 50%) with two lipid levels (6 and 12%) and 2% of digestible carbohydrates were formulated. Fish were fed for 60 days to apparent satiation at a stocking density of 10 fish per tank (100 L). Growth gain of fish fed 50% dietary protein was higher than of fish fed 40% dietary protein (P < 0.05). However, feed efficiency was significantly higher at 12 than 6% of dietary lipid. Whole-body lipid and glycogen in the liver increased dramatically with dietary lipid content-alkaline protease activity trend increased as dietary protein increased. Trypsin activity increased significantly as dietary lipid decreased, whereas chymotrypsin activity showed the opposite trend (P < 0.05). Bile salt-dependent lipase activity trend towards increasing as dietary energy decreased. Glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH), and fructose-1, 6-biphosphatase (FBPase) significantly increased with increasing dietary lipid levels. However, alanine aminotransferase (ALT) activity increased with dietary protein content. On the other side, pyruvate kinase (PK) activity increased with both dietary protein and lipid content. This study indicates that high dietary lipid (12%) improved the feed efficiency but did not reduce dietary protein demand in juveniles O. chrysurus.

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Section: Proximate Analysismentioning

confidence: 99%

Evaluation of protein: lipid ratio on growth, feed efficiency, and metabolic response in juvenile yellowtail snapper Ocyurus chrysurus (Bloch, 1791)

Arenas

Álvarez-González²,

Barreto

et al. 2021

Lat. Am. J. Aquat. Res.

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show abstract

“…In order to evaluate the toxic effects exerted by the mixture, untreated larvae of these populations were also proceeded in this way. The supernatants were used for the estimation of trehalose through anthrone method described by Carroll et al (1956), soluble proteins according to Lowry et al (1951) and glucose contents by O-toluidine method of Hartel et al (1969). The method adopted for the estimation of lipids, was of Zollner and Kirsch (1962) and free amino acid contents was quantified according to Moore and Stein (1954) by using ethanolic extract of larvae.…”

Section: Biochemical Assaymentioning

confidence: 99%

Efficacy of Diatomaceous Earth and Deltamethrin Alone and in Combinations on Mortality and Energy Reserves of Insecticide Resistant Strains of Stored Grain Pest, Trogoderma granarium

Kousar¹,

Riaz²,

Feroz³

et al. 2021

PJZ

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“…Liver glycogen content was determined using the method reported by Carroll et al (1956). Triglycerides were analysed by the enzymatic colorimetric method using a commercial kit (ELITechGroup).…”

Section: Methodsmentioning

confidence: 99%

Physiological and metabolic protein‐sparing effects of dietary lipids on common snookCentropomus undecimalis(Bloch, 1792) juveniles

Arenas

Álvarez‐González

Barreto³

et al. 2021

Aquacult Nutr

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Carnivorous common snook (Centropomus undecimalis) has potential for aquaculture, but its protein requirement in diet is 500 g kg−1 and the energetic role of lipids in this species has not been explored. Thus, this study investigated the ability of juvenile common snook (8.7 ± 1.2 g) to use dietary lipids as an energy source. The fish were fed four practical diets with two protein levels (400 and 500 g kg−1) associated with two lipid levels (60 and 120 g kg−1) for 12 weeks. Growth performance significantly improved with increasing dietary lipid levels, but no significant difference was observed at the protein level. Fish fed the 400:60 (protein: lipid level) diet exhibited significantly higher feed conversion ratios than those fed 400:120, 500:60 and 500:120 diets, but fish fed the 400:120 diet had significantly higher protein efficiency ratios. Juvenile common snook showed the ability to modulate digestive protease and lipase secretion when dietary protein or lipid levels were modified. The activity of alanine aminotransferase decreased as the dietary lipid level increased (p < .05), while fructose‐1 and 6‐bisphosphatase activities were not significantly affected by dietary protein or lipid levels. Glucose‐6‐phosphate dehydrogenase, 6‐phosphogluconate dehydrogenase and pyruvate kinase activities increased as dietary protein and lipid levels increased (p < .05). The results of this study indicate that an increase in dietary lipid level has a protein‐sparing effect and that the diet containing 400 g kg−1 protein with 120 g kg−1 lipid is suitable for growth and effective protein utilization in common snook.

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The Determination of Glycogen in Liver and Muscle by Use of Anthrone Reagent

Cited by 1,109 publications

References 9 publications

Evaluation of protein: lipid ratio on growth, feed efficiency, and metabolic response in juvenile yellowtail snapper Ocyurus chrysurus (Bloch, 1791)

Evaluation of protein: lipid ratio on growth, feed efficiency, and metabolic response in juvenile yellowtail snapper Ocyurus chrysurus (Bloch, 1791)

Efficacy of Diatomaceous Earth and Deltamethrin Alone and in Combinations on Mortality and Energy Reserves of Insecticide Resistant Strains of Stored Grain Pest, Trogoderma granarium

Physiological and metabolic protein‐sparing effects of dietary lipids on common snookCentropomus undecimalis(Bloch, 1792) juveniles

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