The Determination of Hexosamines According to Elson and Morgan.

Blix, Gunnar; Synge, R. L. M.; Eskola, Salli; Lång, Sylvi; Turunen, Elli; Tuhkanen, Aarne

doi:10.3891/acta.chem.scand.02-0467

Cited by 600 publications

(105 citation statements)

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“…Aminosugar was measured by the method of Elson-Morgan as modified by Blix (1948). Ninhydrin-positive substance was estimated as described by Yemm and Cocking (1955).…”

Section: Methodsmentioning

confidence: 99%

Chemical analyses and antitumor activity of hydrosoluble substances from Mycobacterium bovis, strain BCG.

Sato

Arai

Nagai

et al. 1983

Tohoku J. Exp. Med.

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--Hydrosoluble substances from BCG were prepared by cold water extraction and by hot phenol-water extraction. Chemical analyses revealed that both of them were derived from cytoplasm. The cold water extract (CWE) was effective in the treatment of C3H/He mice which had received an intraperitoneal inoculation of a syngeneic ascites hepatoma, MH134. The growth of a graft in footpad of mastocytoma P815 in CDF1 mice was retarded by intraperitoneal injections of CWE. A peptidoglycan from cell wall prepared by digestion with lysozyme exerted no antitumor activity in the same experimental condition as for the evaluation of antitumor effect of CWE. These results indicate that the antitumor activity of CWE was not due to the presence of a cell wall component, ----hydrosoluble substance; BCG; antitumor activity; mastocytoma P815There are a number of publications dealing with the effects of mycobacteria on immune response and on antitumor activity. Several fractions of mycobacteria have been investigated in order to identify the chemical constituents which are responsible for these activities and as a result cell wall has been found to have these biological activities.A hot water extract from delipidated BCG, which has been found to be derived from cytoplasm, is adjuvant-active and exhibits weak but significant antitumor activity (Sato et al. 1976(Sato et al. , 1978(Sato et al. , 1979.In this study, a cold water extract from delipidated BCG and a phenolwater extract were prepared to compare their antitumor effects with those of a water soluble substance from cell wall isolated by an enzymatic treatment of BCG.This report deals with the chemical analyses and antitumor activity of the above hydrosoluble substances.

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“…Aminosugar was measured by the method of Elson-Morgan as modified by Blix (1948). Ninhydrin-positive substance was estimated as described by Yemm and Cocking (1955).…”

Section: Methodsmentioning

confidence: 99%

Chemical analyses and antitumor activity of hydrosoluble substances from Mycobacterium bovis, strain BCG.

Sato

Arai

Nagai

et al. 1983

Tohoku J. Exp. Med.

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“…Protein was determined by the method of Lowry et al [22], inorganic phosphate according to Martin and Dotty [23], nucleic acids by the procedure of Schneider [24], sialic acid by the method of Warren [25], hexosamines according to Blix [26], and ester groups by the hydroxamate method of Hestrin [27]. …”

Section: Methodsmentioning

confidence: 99%

Enzymic Characterization of Golgi-Rich Fractions from Rat Submaxillary-Sublingual Glands

1972

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Isolation of Golgi-enriched subcellular fractions from rat submaxillar-sublingual glands was achieved by using a combination of differential and discontinuous sucrose-density-gradient centrifugation. Each fraction was characterized by enzyme markers, including three glycosyltransferaseas : galactosyltransferase, N-acetylglucosaminyltransferase, and N-acetylgalactosaminyltransferase. Golgi fractions enriched in the latter two enzymes were further studied using bovine, porcine, and rat submaxillary mucins and their trifluoroacetic-acid-treated materials as acceptors. Treatment of the intact mucins with trifluoroacetic acid yielded two protein fractions, one with a hexosamine content of about 15O/,, and the other virtually free of sugars. Only intact bovine m u c h and the hexosamine-containing acceptor protein were efficient substrates for either hexosaminyl transfer. Ultrafilterable peptides derived from rat submaxillary-sublingual glands showed high acceptor activity for both N-acetylhexosamines in the presence of Golgi-rich fraction 2.

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“…Total hexosamine was quantitatively determined, after hydrolysis of the samples in 4 N-HCl for 4 hr a t 110", by the Elson-Morgan reaction (Blix, 1948), with glucosamine as standard.…”

Section: Methodsmentioning

confidence: 99%