The determination of iron in blood plasma or serum

Ramsay, W. N. M.

doi:10.1016/0009-8981(57)90105-5

Cited by 332 publications

(56 citation statements)

References 16 publications

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“…Protein was estimated by means of the biuret test. Iron was estimated by the method in [23]. Activities of enzymes were expressed in % of the sum of activities in the subfractions and as specific activities (spec.…”

Section: Enzyme Assaysmentioning

confidence: 99%

Submitochondrial localization of transferrin and iron accumulated by isolated rat liver mitochondria

Konopka

Turska

1979

FEBS Letters

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Section: Enzyme Assaysmentioning

confidence: 99%

Submitochondrial localization of transferrin and iron accumulated by isolated rat liver mitochondria

Konopka

Turska

1979

FEBS Letters

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“…In experiments where several plasmas were utilized the fraction of medium "9Fe incorporated into heme was multiplied by the total iron in the medium to calculate the picograms of iron incorporated into heme. The medium iron was derived entirely from plasma and serum and was determined by the method of Ramsay (30).…”

Section: Case Reportmentioning

confidence: 99%

Studies on Red Cell Aplasia. V. PRESENCE OF ERYTHROBLAST CYTOTOXICITY IN γG-GLOBULIN FRACTION OF PLASMA

Krantz¹,

Moore²,

Zaentz³

1973

J. Clin. Invest.

128

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A B S T R A C T The marrow cells of a patient with pure red cell aplasia markedly increased their rate of heme synthesis when they were freed from the host environment and were incubated in vitro. When the red cell aplasia was treated with cyclophosphamide and prednisone, marrow cell incorporation of 'Fe into heme in vitro increased several weeks before a reticulocytosis was apparent, and was the earliest effect noted. The plasma 'YG-globulins of this patient inhibited heme synthesis by normal marrow cells or the patient's own marrow cells obtained after remission of the disease.Since the inhibition of heme synthesis could be the result of damage to erythroblasts, the patient's posttreatment marrow cells or normal marrow cells were labeled with '9Fe and were then incubated with the patient's pretreatment, treatment, and posttreatment ,YG-globulins as well as normal YG-globulins. At the end of this incubation the supernatant and cells were separated and counted. Heme was extracted and also was counted. Treatment of the cells with the patient's pretreatment YG-globulins resulted in a release of 40% of the radioactive heme from the cells. This represented the loss of radioactive hemoglobin and was an index of erythroblast cytotoxicity. A progressive disappearance of the cytotoxic factor in the YG-globulins occurred in the 3 wk period preceding the onset of reticulocytes in the patient's blood. Posttreatment and normal yG-globulins did not produce this effect and increased injury of red cells and lymphocytes was not produced by the patient's pretreatment yG-globulins. These studies demonstrate a method for measuring erythroblast cytoxicity and show that red cell aplasia is associated with YG-globulins that This study was presented in part at

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“…Ethanolamine and methoxyethanol (1: 2 mixture) were added 2Fisher Scientific Company, Fair Lawn, N. J. 3 Pentex Inc., Kankakee, Ill. [25][26][27][28][29][30] to absorb C02 and an aliquot was mixed with scintillator solution 4 for counting in a well-type scintillation spectrometer. 5 Toluene-"C was used as internal standard; counting efficien-y was 30-40%.…”

Section: Introductionmentioning

confidence: 99%

Carbon Monoxide Production Associated with Ineffective Erythropoiesis*

White¹,

Coburn²,

Williams³

et al. 1967

J. Clin. Invest.

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Abstract. The rate of endogenous carbon monoxide production (Vco), determined by the closed rebreathing system technique, was elevated above the normal range in four of five patients studied with ineffective erythropoiesis (four patients with primary refractory anemia, one with thalassemia). The mean molar ratio of Vco to Vheme (rate of circulating heme catabolism, determined from 51Cr red cell survival curves) was 3.0 ± 0.6 (SE), indicating that most of the CO originated from sources other than circulating erythrocyte hemoglobin, in contrast to previous findings in patients with hemolytic anemia, where Vco paralleled Vheme closely.After administration of glycine-2-4C to these patients, endogenous CO was isolated by washout of body CO stores at high PO2 or by reacting peripheral venous blood samples with ferricyanide. The CO was then oxidized to CO2 by palladium chloride and trapped for counting in a liquid scintillation spectrometer.

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The determination of iron in blood plasma or serum

Cited by 332 publications

References 16 publications

Submitochondrial localization of transferrin and iron accumulated by isolated rat liver mitochondria

Submitochondrial localization of transferrin and iron accumulated by isolated rat liver mitochondria

Studies on Red Cell Aplasia. V. PRESENCE OF ERYTHROBLAST CYTOTOXICITY IN γG-GLOBULIN FRACTION OF PLASMA

Carbon Monoxide Production Associated with Ineffective Erythropoiesis*

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