The Determination of Parkinson’s Drugs in Human Urine by Applying Chemometric Methods

Abstract

The spectrophotometric-chemometric analysis of levodopa and carbidopa that are used for Parkinson’s disease was analyzed without any prior reservation. Parkinson’s drugs in the urine sample of a healthy person (never used drugs in his life) were analyzed at the same time spectrophotometrically. The chemometric methods used were partial least squares regression (PLS) and principal component regression (PCR). PLS and PCR were successfully applied as chemometric determination of levodopa and carbidopa in human urine samples. A concentration set including binary mixtures of levodopa and carbidopa in 15 different combinations was randomly prepared in acetate buffer (pH 3.5).). UV spectrophotometry is a relatively inexpensive, reliable, and less time-consuming method. Minitab program was used for absorbance and concentration values. The normalization values for each active substance were good (r2>0.9997). Additionally, experimental data were validated statistically. The results of the analyses of the results revealed high recoveries and low standard deviations. Hence, the results encouraged us to apply the method to drug analysis. The proposed methods are highly sensitive and precise, and therefore they were implemented for the determination of the active substances in the urine sample of a healthy person in triumph.

A Chemometric Analysis of Montelukast Sodium And Levocetirizine Dihydrochloride In Pharmaceutical Formulations Using Artificial Neural Networks, Partial Least Squares, And Principal Component Regression Models

“…Partial Least Squares [32][33][34][35][36] and Principal component Regression [37][38] are regression based multivariate statistical tools which have more advantages over univariate model and can be used for multicomponent analysis. Theoretical background of these methods are covered in details in literature [39][40] PCR and PLS need a calibration step where the models for the spectra and the component concentrations of the unknown are estimated from the sample spectrum.…”

Pharmacokinetic Study of Zandopa® in Rat Model

“…The dilution procedure at the experimental stage was taken into account in the calculations. Thus, the data collected was the volume of urine collected and the drug concentration in urine during each interval [12,13] . The rate of excretion and the total amount excreted were estimated after analysing urine data (Table 1).…”