“…Vacuum settings were altered to change flow rates, which were measured by determining the amount of time required for solutions to completely flow through each disk. The following flow rates were measured: 8,9,48,174,193,212, and 290 mL/min, and three at maximum vacuum, resulting in a 348 mL/min flow rate. The discs were rinsed with 40 mL of 0.1M nitric acid and added to Perkin Elmer Ultima Gold TM AB LSC cocktail.…”