The determination of plasma fibrinogen by turbidity with ammonium sulfate

Parfentjev, I. A.; Johnson, Muriel; Cliffton, Eugene E.

doi:10.1016/0003-9861(53)90218-5

Cited by 86 publications

(19 citation statements)

References 16 publications

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“…The one-stage method of Quick (1966) was used to assess the prothrombin time (PT). The activated partial thromboplastin time was performed with Celiteactivated phospholipid (Nye & Graham, 1962) and fibrinogen was measured turbidometrically (Parfentjev, Johnson & Clifton, 1953). Quantitation of the fibrin-split products was carried out by the Staphylococcal clumping test (Hawiger, Niewearoski, Gusewich & Thomas, 1970).…”

Section: Blood Analysismentioning

confidence: 99%

E. col/ ENDOTOXIN SHOCK IN THE DOG; TREATMENT WITH LIDOCAINE OR INDOMETHACIN

Fletcher

Ramwell

1978

British J Pharmacology

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1 Dogs treated with lidocaine (1 mg kg-' h-1) or indomethacin (1.5 mg/kg) before and after an LD60 dose (1 mg/kg) of E. coli endotoxin survived for at least 72 h.2 Although all dogs in both treated groups survived, only those treated with indomethacin were significantly protected against the fall in the arterial blood pressure 1 to 2 min following endotoxin administration.3 Endotoxin increased the plasma prostaglandin F2Q (PGF2,) concentration in the control and lidocaine-ireated groups, however, no increase was observed with indomethacin treatment. 4 Neither lidocaine nor indomethacin alone had any significant effect on the parameters measured in this model.5 Following the administration of endotoxin, lidocaine-treated animals had significantly decreased plasma fibrinogen concentrations when compared to the other groups.6 This study suggests that lidocaine, a local anaesthetic and a drug widely used for cardiac arrhythmias, might offer protection in endotoxin shock. ImtrodUctiomPrevious work in this laboratory (Fletcher, Herman & Ramwell, 1976a;Fletcher, Ramwell & Herman, 1976b;Fletcher & Ramwell, 1977) has documented increased plasma prostaglandin concentrations following the administration of endotoxin intravenously in dogs and primates. The increases in prostglandins were related in time to changes in circulatory function. Therapeutic doses of analgesic-antipyretic drugs given before and after administration of endotoxin diminished the early haemodynamic changes and prevented the increase in the prostaglandins, but failed to improve the survival in the lethal (LD1oo) baboon model. In contrast, in the LD50 dog model, these drugs did improve the survival and the early circulatory function while inhibiting the synthesis of the prostaglandins. Although the prostaglandins were elevated as early as 1 to 2 min after the endotoxin administration, their relationship to the pathophysiology of endotoxin shock is unclear.The analgesic-antipyretic drugs do improve circulatory function and survival in canine endotoxin shock but the exact mechanism by which they exert a beneficial effect is unknown. We demonstrated (Fletcher & Ramwell, 1977) that while indomethacin or aspirin

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Section: Blood Analysismentioning

confidence: 99%

E. col/ ENDOTOXIN SHOCK IN THE DOG; TREATMENT WITH LIDOCAINE OR INDOMETHACIN

Fletcher

Ramwell

1978

British J Pharmacology

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“…Blood levels of these potent vasoactive fatty acids are increased in endotoxin shock (Anderson, Jubiz, Fralios, Tsagaris & Kuida, 1972;Kessler, Hughes, Bennett & Nadela, 1973;Anderson et al, 1975a,b;Fletcher et al, 1976), and are related in time to the haemodynamic events (Fletcher etal., 1976 Quick (1966) was used to assess the prothrombin time. The activated partial thromboplastin time was performed with Celite-activated phospholipid (Nye & Graham, 1962) and fibrinogen was measured turbidometrically (Parfentjev, Johnson & Clifton, 1953). Quantitation of the fibrin-split products was by means of the Staphylococcal clumping test (Hawiger, Niewearoski, Gusewich & Thomas, 1970).…”

Section: Introductionmentioning

confidence: 99%

MODIFICATION, BY ASPIRIN AND INDOMETHACIN, OF THE HAEMODYNAMIC AND PROSTAGLANDIN RELEASING EFFECTS OF E. coli ENDOTOXIN IN THE DOG

Fletcher

Ramwell

1977

British J Pharmacology

100

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Dogs treated with aspirin (10 mg/kg) or indomethacin (1.5 mg/kg) 45 min before, and 3 h after, an LD50 dose (1 mg/kg) of E. coli endotoxin were alive 72 h later. Although all dogs in both treated groups survived, only those treated with indomethacin were protected against the fall in blood pressure 1–2 min following endotoxin. Endotoxin increased the level of prostaglandin F2α in both the mixed venous and arterial blood. No increase was observed in the aspirin and indomethacin‐treated groups. Aspirin and indomethacin treatment did not modify thrombocytopaenia or blood coagulation parameters following endotoxin.

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“…Fibrinogen was isolated from the plasma by an ammonium sulfate procedure (12) and the protein content of the precipitate determined (9). The albumin and globulins were separated clectrophoretically on Sepraphore III cellulose polyacetate strips.…”

Section: Methodsmentioning

confidence: 99%

The Pharmacological Properties of Seclazone (7-chloro-3,3a-dihydro-2H, 9H-isoxazolo [3,2-b] [1,3] benzoxazin-9-one) A New Anti-Inflammatory Agent

Berger¹,

Bates²,

Diamantis³

et al. 1973

Pharmacology

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Seclazone possesses anti-inflammatory, analgesic and antipyretic properties. It effectively suppresses the development of adjuvant-induced polyarthritis in rats and the serum protein alterations associated with this condition. Seclazone also prevents phlogistic responses induced in rats by carrageenan. The anti-inflammatory effect of seclazone is not mediated by adrenocortical or pituitary stimulation since activity persists in adrenalectomized or hypophysectomized animals. Seclazone has an appreciable anti-bradykinin action in vitro. The drug is free of ulcerogenic activity at therapeutically active levels and possesses diuretic properties.

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The determination of plasma fibrinogen by turbidity with ammonium sulfate

Cited by 86 publications

References 16 publications

E. col/ ENDOTOXIN SHOCK IN THE DOG; TREATMENT WITH LIDOCAINE OR INDOMETHACIN

E. col/ ENDOTOXIN SHOCK IN THE DOG; TREATMENT WITH LIDOCAINE OR INDOMETHACIN

MODIFICATION, BY ASPIRIN AND INDOMETHACIN, OF THE HAEMODYNAMIC AND PROSTAGLANDIN RELEASING EFFECTS OF E. coli ENDOTOXIN IN THE DOG

The Pharmacological Properties of Seclazone (7-chloro-3,3a-dihydro-2H, 9H-isoxazolo [3,2-b] [1,3] benzoxazin-9-one) A New Anti-Inflammatory Agent

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