The determination of polychlorodibenzo-<i>p</i>-dioxins in pentachlorophenol and wood treatment solutions

Lamberton, J. G.; Griffin, D. C.; Arbogast, Brian; Inman, Roderick D.; Deinzer, Max L.

doi:10.1080/15298667991430343

Cited by 19 publications

(5 citation statements)

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“…Control solutions of peanut oil were prepared in a similar manner but without TCDD. The concentration of TCDD in each solution was confirmed by gas chromatography using a modified method of Laberton et al [30]. Animals were treated with peanut oil (vehicle) or TCDD at various doses by gavage using volumes of 0.01 mL/g body weight.…”

Section: Methodsmentioning

confidence: 99%

2,3,7,8-Tetrachlorodibenzo-p-dioxin Slows the Progression of Experimental Cutaneous Leishmaniasis in Susceptible BALB/c and SCID Mice

et al. 2013

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In a model of experimental cutaneous leishmaniasis, pre-exposure of Leishmania major-resistant mice to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), an aryl hydrocarbon receptor agonist, causes suppression of the protective anti-parasite T helper 1 response while paradoxically also reducing parasite burdens in those animals. In this study, we examined if TCDD exposure could also reduce parasite burdens in L. major-susceptible BALB/c mice. In the highest dose group (160 µg/Kg), TCDD treatment caused a significant reduction of parasite burdens by 10-fold after three weeks while also causing a significant lymphoid atrophy indicating suppression of the non-protective T helper 2 response. A dose-dependent delay of foot lesion progression was also observed such that lesion size in the highest dose group was less than half that of controls after 35 days of infection. Importantly, although TCDD exposure initially reduced disease severity and prolonged the course of disease by as much as three fold in some animals, this effect was transitory and TCDD did not induce resistance to L. major infection. Because TCDD exposure reduced L. major burdens in both resistant and susceptible mice, we hypothesized that TCDD reduces L. major burdens in mice by a mechanism that does not involve adaptive immunity. To test this, severe combined immunodeficient (SCID) mice were used. In mice infected with a moderate number of L. major (10,000), TCDD treatment caused a time- and dose-dependent decrease of parasite burdens by nearly 100-fold after six weeks in the highest dose group (200 µg/Kg). A significant and dose-dependent delay of foot lesion progression was also observed in these animals. These results indicate that TCDD exposure can reduce the severity of leishmanial disease in mice independent of adaptive immunity.

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Section: Methodsmentioning

confidence: 99%

2,3,7,8-Tetrachlorodibenzo-p-dioxin Slows the Progression of Experimental Cutaneous Leishmaniasis in Susceptible BALB/c and SCID Mice

et al. 2013

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“…The concentration of TCDD was confirmed by gas chromatography using the method of Laberton et al [12]. …”

Section: Methodsmentioning

confidence: 99%

The growth and infectivity of Leishmania major is not altered by in vitro exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin

Sazhnev

DeKrey

2018

BMC Res Notes

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ObjectiveThe numbers of Leishmania major parasites in foot lesions of C57Bl/6, BALB/c or SCID mice can be significantly reduced by pre-exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). One potential mechanism to explain this enhanced resistance to infection is that TCDD is directly toxic to L. major. This potential mechanism was addressed by exposing L. major promastigotes and amastigotes to TCDD in vitro and examining their subsequent proliferation and infectivity.ResultsWe found no significant change in the rate of in vitro L. major proliferation (promastigotes or amastigotes) after TCDD exposure at concentrations up to 100 nM. Moreover, in vitro TCDD exposure did not significantly alter the ability of L. major to infect mice, trigger lesion formation, or survive in those lesions.

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“…TCDD was purchased from Cambridge Isotope Laboratories (Andover, MA) and solutions were prepared in peanut oil as described previously [11]. The concentration of TCDD was confirmed by gas chromatography using the method of [30]. Each animal (36 total, 18 of each sex, 6 per treatment group within each sex) was randomly assigned to a treatment group and was given peanut oil (vehicle) or TCDD (in peanut oil) at one of various doses by gavage (0.01 mL/g body weight).…”

Section: Treatments and Infectionsmentioning

confidence: 99%

TCDD exposure alters fecal IgA concentrations in male and female mice

Foxx

Nagy

King

et al. 2022

BMC Pharmacol Toxicol

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Background Activation of the aryl hydrocarbon receptor (AhR) can alter diurnal rhythms including those for innate lymphoid cell numbers, cytokine and hormone levels, and feeding behaviors. Because immune responses and antibody levels are modulated by exposure to AhR agonists, we hypothesized that some of the variation previously reported for the effects of AhR activation on fecal secretory immunoglobulin A (sIgA) levels could be explained by dysregulation of the diurnal sIgA rhythm. Methods C57Bl/6 J mice were exposed to peanut oil or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, 10 or 40 μg/Kg) and fecal sIgA levels were determined in samples collected every 4 h over 4 days. Results Fecal sIgA concentrations were not significantly different between light and dark phases of the photoperiod in either male or female mice, and there were no significant circadian rhythms observed, but TCDD exposure significantly altered both fecal mesor sIgA and serum IgA concentrations, in parallel, in male (increased) and female (biphasic) mice. Conclusions AhR activation can contribute to the regulation of steady state IgA/sIgA concentrations.

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The determination of polychlorodibenzo-p-dioxins in pentachlorophenol and wood treatment solutions

Cited by 19 publications

References 0 publications

2,3,7,8-Tetrachlorodibenzo-p-dioxin Slows the Progression of Experimental Cutaneous Leishmaniasis in Susceptible BALB/c and SCID Mice

2,3,7,8-Tetrachlorodibenzo-p-dioxin Slows the Progression of Experimental Cutaneous Leishmaniasis in Susceptible BALB/c and SCID Mice

The growth and infectivity of Leishmania major is not altered by in vitro exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin

TCDD exposure alters fecal IgA concentrations in male and female mice

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