The Determination of Presence of Listeria monocytogenes in Ground Meat Sold in Istanbul

Abstract

Among the 21 different species of the Listeria genus, Listeria monocytogenes is the most common and listeriosis agent in humans. The mortality rate of L. monocytogenes infection is higher than the other common foodborne pathogens such as Salmonella Enteritidis, Campylobacter and Vibrio species. In the current study, it is aimed to determine the presence of L. monocytogenes bacteria in minced meat samples sold in Istanbul province by using the culture method of the USDA-FSIS. In this study, 100 minced meat samples purchased from different butchers in 11 districts of Istanbul between December 2018 and November 2019 were examined for the presence of L. monocytogenes. The bacteria identified also molecularly verified according to the presence of iap and hlyA gene regions by the polymerase chain reaction (PCR) method. When antibiotic susceptibility tests of 21 strains, identified as L. monocytogenes through biochemical tests, it was found to be resistant to Amoxicillin/Clavulanic acid (14.28%), penicillin (9.52%), cefaclor (9.52%), vancomycin (9.52%), ciprofloxacin (9.52%) and trimethoprim-sulfamethoxazole (9.52%) and susceptible to ampicillin (100%) and tetracycline (100%). In terms of iap and hlyA gene regions, only 16 of 21 isolates, identified as L. monocytogenes as a result of biochemical tests, were found to be L. monocytogenes. In our study with minced meat offered for sale in Istanbul, although, the L. monocytogenes isolation rate (17%) and the incidence of antibiotic resistance of the isolated L. monocytogenes bacteria are low, it was concluded that minced meat may pose a public health risk.

Antibiotic resistance and biofilm forming abilities of Listeria monocytogenes and effect of subMIC concentration of white vinegar on these virulence factors

“…Our findings are in agreement with data reported on antibiotic resistance of L. monocytogenes isolated from meat and meat products in other studies (Wang et al, 2013;Arslan and Baytur, 2019;Matle et al, 2019;Uluda g et al, 2023). Ampicillin or penicillin G in combination with an aminoglycoside such as gentamicin is a first choice for treating human listeriosis.…”

“…In our study, L. monocytogenes was detected in 9 (30%) out of 30 retail ground meat samples. L. monocytogenes was detected within the range of 12.4-41.9% in raw meat and meat products in recent studies in different regions of Türkiye and in various countries in the world (Wang et al, 2013;Arslan and Baytur, 2019;Matle et al, 2019;Uluda g et al, 2023). The results of these studies showed that the contamination rates of L. monocytogenes in raw meat samples were quite high.…”

Evaluation of Antimicrobial Activity by Marine Nocardiopsis dassonvillei against Foodborne Listeria monocytogenes and Shiga Toxin-Producing Escherichia coli

“…The 21st century is plagued by the emergence of multidrug-resistant bacteria such as foodborne pathogenic strains of Listeria and Shiga toxin-producing Escherichia coli (STEC) [1,2]. The major contributing factors to their antimicrobial resistance include the routine application of antimicrobials in domestic livestock for growth promotion, disease treatment, and prophylaxis [3].…”