The Determination of Sugar in Blood and Spinal Fluid With Anthrone Reagent

Roe, Joseph H.

doi:10.1016/s0021-9258(18)71120-4

Cited by 1,256 publications

(91 citation statements)

References 8 publications

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“…SBLP was detected as a glycoprotein by periodic acid-Schiff staining. This is further confirmed using the method of Roe [18], which indicated a carbohydrate content of 14.0% in SBLP. The carbohydrate content of SBLP is much higher than that of lectins from Brazilian lima bean (2% and 4%) [19,20], rhizome of Alisma orientale (Sam.)…”

supporting

confidence: 62%

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Isolation and Characterization of a Lectin-like Protein (SBLP) from the Dried Roots of Scutellaria baicalensis (Lamiaceae)

Wang

Gao

Liu

et al. 2018

Natural Product Communications

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A novel lectin-like protein with MW 63.2 kDa, designated as SBLP, has been isolated and characterized from the dried roots of Scutellaria baicalensis Georgi (Lamiaceae). SBLP was purified by ammonium sulfate precipitation and anion exchange chromatography. It is a glycoprotein according to a PAS staining assay and consisting of protein (86.0%) and sugar (14.0%). Its N-terminal amino acid sequence was determined as GSAVGFLY by Edman degradation. SBLP showed hemagglutinating activity against human and rooster erythrocytes, which were stable below 60°C and in the pH range of 4-10. Furthermore, SBLP was found to be stimulated by Ca 2+ , Na + , Ba 2+ , Zn 2+ ions, which suggested it was a metal-dependent lectin. SBLP inhibited the growth of Fusarium oxysporum f.sp. lycopersici and Alternaria eichhorniae in the a dose-dependent manner, and suppressed the proliferation of HepG2 tumor cells with an IC 50 of 1.00 µM. This is the first report of a lectin from Radix Scutellariae.

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supporting

confidence: 62%

“…The anthrone-sulfuric acid method was applied to determine the total carbohydrate. Glucose was used as the standard [18].…”

Section: Protein Concentration and Sugar Concentration Determinationmentioning

confidence: 99%

Isolation and Characterization of a Lectin-like Protein (SBLP) from the Dried Roots of Scutellaria baicalensis (Lamiaceae)

Wang

Gao

Liu

et al. 2018

Natural Product Communications

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“…The proline content in fresh leaves was ascertained by using a method by Bates (1973). Total soluble sugar also was assayed using anthrone reagent and glucose as standard (Roe 1955).…”

Section: Carbohydrates and Proline Concentration Analysismentioning

confidence: 99%

The effects of foliar application of melatonin on some physiological and biochemical characteristics and expression of fatty acid desaturase gene in pistachio seedlings (Pistacia veraL.) under freezing stress

Barand

Nasibi

Kalantari

et al. 2020

Journal of Plant Interactions

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In this study, the impacts of exogenous melatonin on freezing stress mitigation in pistachio (Pistacia vera L.) seedlings (30-days old) were investigated. Our results showed that in pistachio seedlings under freezing stress antioxidant enzyme activity, malondialdehyde (MDA) hydrogen peroxide (H 2 O 2 ), electrolyte leakage, chlorophyll degradation, proline and soluble sugar and γ-aminobutyric acid (GABA) content increased. Phenolic component, phenylalanine ammonia lyase (PAL) activity and the expression of fatty acid desaturase (FAD2) gene increased whilst ascorbate and glutathione concentration significantly decreased in comparison with control. In this research, application of melatonin significantly reduced the antioxidant enzyme activity, MDA, H 2 O 2 , chlorophyll degradation, GABA concentration, soluble sugar, and proline contents under stress condition. In addition electrolyte leakage, PAL activity and transcripts of FAD2 gene markedly decreased when the leaves of pistachio seedlings sprayed with melatonin. The results of this study showed that MEL could alleviate the oxidative damages induced by freezing stress and to maintain the structure and mobility of cell membrane in pistachio seedlings under stress conditions. Considering that MEL is an inexpensive and safe bio-stimulator, it seems that MEL is a potential candidate that could be applied used to increase the resistant pistachio trees against variety of biotic and abiotic stresses in the field conditions.

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“…Enzymatic and colorimetric analyses adapted for small micro plates (Palacios et al, 1998) were performed, and the quantification of P was realized (Bradford, 1976) after digestion in 0.1 N NaOH. Carbone was quantified by the Anthrone method, after precipitation of proteins with trichloroacetic acid (Roe, 1955). Lipids were measured according to the sulfophosphovanillin method (Barnes & Blackstock, 1973) and Tg was quantified using a kit (GOD-PAP, Merck).…”

Section: Egg Biochemical Analysesmentioning

confidence: 99%

Reproductive broodstock performance and egg quality of wild-caught and first-generation domesticated Seriola rivoliana reared under same culture conditions

Quiñones-Arreola

Arcos-Ortega²,

Gracia‐López³

et al. 2017

Lat. Am. J. Aquat. Res.

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Almaco jack, Seriola rivoliana as well as some related species is of great interest in marine fish aquaculture. However, there are few studies about their reproduction in captivity. In this research work, reproductive performance and egg quality in two groups of adult Seriola rivoliana, caught in the wild and domesticated-F1 analyzed and compared, reared under optimal maturation conditions in a commercial private Laboratory. A total of 28 wild adult (>5 kg) were caught at La Paz Bay, Baja California Sur, Mexico, and 30 adult domesticated-F1 broodstock (>5 kg), were obtained from an original stock of 1,000 juveniles (3.5 g body weight) produced at Kona Blue (Hawaii, USA) sea farm. Fishes were transported to the Rancheros del Mar commercial private hatchery, where they were grown to adult size. Both groups were evaluated during eight months (May to December 2012) and compared in terms of reproduction performance (total number of spawning events, monthly spawning frequency, total number of eggs, total number of eggs per mL, and fertilization rate), egg biochemical composition (total proteins, total lipids, total carbohydrates, and triacylglycerides) and egg diameter. Results indicated that wild caught broostock showed a better reproductive performance in terms of fertilization rate, total number of spawning, monthly spawning frequency and total number of eggs produced. However, biochemical composition and egg diameter did not show statistical differences (P < 0.05) between two groups. The reproductive performance of broodstock and quality of eggs analyzed in this study are important traits to improve the aquaculture management of this species.

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The Determination of Sugar in Blood and Spinal Fluid With Anthrone Reagent

Cited by 1,256 publications

References 8 publications

Isolation and Characterization of a Lectin-like Protein (SBLP) from the Dried Roots of Scutellaria baicalensis (Lamiaceae)

Isolation and Characterization of a Lectin-like Protein (SBLP) from the Dried Roots of Scutellaria baicalensis (Lamiaceae)

The effects of foliar application of melatonin on some physiological and biochemical characteristics and expression of fatty acid desaturase gene in pistachio seedlings (Pistacia veraL.) under freezing stress

Reproductive broodstock performance and egg quality of wild-caught and first-generation domesticated Seriola rivoliana reared under same culture conditions

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